2013 Fiscal Year Final Research Report
Analysis of collective cell migration by using zebrafish in vivo imaging
| Project/Area Number |
22657048
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Chiba University (2012-2013)
Nagoya University (2010-2011) |
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Principal Investigator |
ITOH MOTOYUKI 千葉大学, 薬学研究科(研究院), 教授 (20377906)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Koichi 国立遺伝学研究所, 教授 (70195048)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | 細胞骨格・運動 / アンチセンス |
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| Research Abstract |
Collective cell migration plays a role in morphogenesis during development and cancer cell metastasis. However molecular mechanisms underlying its regulation remain uncertain. We used zebrafish lateral line system as a model for collective cell migration and established transgenic lines that express imaging reporters for actin and microtubule dynamics. Furthermore, BNA-modified antisense oligonucleotides was evaluated for their efficacy in gene knockdown in zebrafish. We show a potential application for BNA antisense oligos in the downregulation of specific gene expression in zebrafish.
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