Reveal the mechanism of eukaryotic cell-on the base of genome information of mitochondrial division apparatus

2011 Fiscal Year Final Research Report

• Project/Area Number: 22657061
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Rikkyo University
• Principal Investigator: KUROIWA Tsuneyoshi 立教大学, 理学研究科, 特任教授 (50033353)
• Project Period (FY): 2010 – 2011
• Keywords: 真核生物起源 / 植物ゲノム

Research Abstract

On the basis of 100% genome information of the primitive red alga, which we already decoded(Matsuzaki et al. Nature 428, 653, 2004), we elucidated all proteins and their genes in the isolated mitochondrial division apparatus by using the MALDI-TOF MS.(Yoshida et al. Science 313、1435, 2006). We have succeeded to elucidate the mechanism of the birth of eukaryotic cells from the perspective of the regulation of symbiotic bacteria by the host cell using the genome the information.

Research Products

• [Journal Article] A novel ribosome dimerization factor(RDF), YvyD, in Bacillus subtilis (2012)
  • Author(s): Tagami K., Nanamiya H., Murakami K., Maehashi M., Masuda K., Natori Y., Tozawa Y., Yoshida M., Kuroiwa H., Kuroiwa T. and Kawamura F
  • Journal Title: Microbiol. Open
  • Peer Reviewed
• [Journal Article] Mitotic inheritance of ER in the primitive red alga Cyanidioschyzon merolae (2012)
  • Author(s): Yagisawa F., Fujiwara T., Ohnma M., Imoto, Y., Yoshida Y., Kuroiwa H., and Kuroiwa T.
  • Journal Title: Protoplasma
  • Peer Reviewed
• [Journal Article] Aurora kinase of the red alga Cyanidioschyzon merolae is related to both mitochondrial division and mitotic spindle formation (2012)
  • Author(s): Kato S., Imoto U., Ohnuma M., Matsunaga T. M., Kuroiwa H., Kawano S., Kuroiwa T. and Matsunaga S
  • Journal Title: Cytologia Volume: 76 Pages: 455-462
  • Peer Reviewed
• [Journal Article] Involvement of elongation factor-1α, but not an actomyosin contractile ring, in cytokinesis in the primitive red alga Cyanidioschyzon merolae (2012)
  • Author(s): Imoto U., Nishida K., Yagisawa F., Yoshida Y., Ohnuma M., Yoshida M., Fujiwara T., Kuroiwa H., Kawano S. and Kuroiwa T.
  • Journal Title: Cytologia Volume: 76 Pages: 431-437
  • Peer Reviewed
• [Journal Article] Cytological aspect of cell cycle and organelle dividing cycle (2011)
  • Author(s): Imoto Y., Yagisawa, F., Yoshida, Y., Kuroiwa, H., and Kuroiwa T.
  • Journal Title: J. Electron Microscopy Volume: 60 Pages: S117-S136
  • Peer Reviewed
• [Journal Article] Optimization of cryopreservation conditions for the unicellular red alga Cyanidioschyzon merolae (2011)
  • Author(s): Ohnuma M., Kuroiwa T and Tanaka K.
  • Journal Title: J. Gen. Appl. Microbiol. Volume: 578 Pages: 137-143
  • Peer Reviewed
• [Journal Article] Proteomic comparison between interphase and metaphase of isolated chloroplasts of Cyanidioschyzon merolae(Cyanidiophyceae, Rhodophyta) (2011)
  • Author(s): Yoshida M., Yoshida, Y., Fujiwara, T., Misumi, O., Kuroiwa, H. and Kuroiwa, T.
  • Journal Title: Phycol. Res Volume: 59 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Phototaxis in the unicellular red algae. Cyanidioschyzon merolae and Cyanidium caldarium (2011)
  • Author(s): Ohnuma M., Misumi O. and Kuroiwa T.
  • Journal Title: Cytologia Volume: 76 Pages: 295-300
  • Peer Reviewed
• [Journal Article] DNA packaging proteins Glom and Hlom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum (2011)
  • Author(s): Itoh K, Izumi A, Mori T, Dohmae N, Yui R, Sano K, Shirai Y, Kanaoka M, Kuroiwa T, Higashiyama T. Murakami-Murofushi K, Kawano S and Sasaki N.
  • Journal Title: Mitochondrion Volume: 11 Pages: 575-586
  • Peer Reviewed
• [Journal Article] The basics of cultivation and molecular genetic analysis of the unicellular red alga Cyanidoschyzon merolae (2010)
  • Author(s): Kobayashi, Y., Ohnuma, M., Kuroiwa, T., Tanaka, K., and Hanaoka, M.
  • Journal Title: J. Endocytobiosis Cell Res Volume: 20 Pages: 53-61
  • Peer Reviewed
• [Journal Article] Plastid division is driven by glycoprotein PDR1 with poly-glucan filaments (2010)
  • Author(s): Yoshida Y., Kuroiwa H., Misumi O., Yoshida M., Ohnuma M. 1, Fujiwara T., Yagisawa F., Hirooka S., Matsushita K., Kawano S. and Kuroiwa., T
  • Journal Title: Science Volume: 329 Pages: 949-953
  • Peer Reviewed
• [Journal Article] The Overexpression of Cyanidioschyzon merolae S-adenosylmethionin synthetase enhances salt tolerance in transgenic Arabidopsis thaliana (2010)
  • Author(s): Sakajiri, T., Asano, K., Hirooka, S., Ohnuma, M., Misumi, O., Yoshida, M., Fujiwara, T., Doi, S., Kuroiwa, H. and Kuroiwa, T
  • Journal Title: Cytologia Volume: 75 Pages: 341-352
  • Peer Reviewed
• [Journal Article] The Functional Domain of GCS1-based Gamete Fusion Resides in the Amino Terminus in Plant and Parasite Species (2010)
  • Author(s): Mori, T., Hirai, M., Kuroiwa, T., and Miyagishima, M. S.
  • Journal Title: Plos one Volume: 5 Pages: e15957
  • Peer Reviewed
• [Journal Article] The vacuole binding to mitochondria by VIG1 contribututes an equal inheritance of the vacuoles in Cyanidioschyzon merolae (2010)
  • Author(s): Fujiwara T., Yagisawa F., Ohnuma M., Yoshida Y., Yoshida M., Nishida K., Misumi O., Kuroiwa H. and Kuroiwa T.
  • Journal Title: Cytologia Volume: 75 Pages: 189-194
  • Peer Reviewed
• [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semi-amylopectin-type, but not an amylose-typeα-glucan (2010)
  • Author(s): Hirabaru C., Izumo A., Fujiwara S., Tadokoro Y., Shimonaga T., Konishi M., Yoshida M., Fujita N., Nakamura Y., Yoshida Y., Kuroiwa T., and Tsuzuki M.
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 682-693
  • Peer Reviewed
• [Journal Article] Origin of eukaryotic cells as deduced from organelle division machineries (2010)
  • Author(s): Kuroiwa T.
  • Journal Title: Proc. Jpn. Acad. Seri. B Volume: 86 Pages: 455-471
  • Peer Reviewed
• [Journal Article] Division and segregation of cell nucleus, mitochondrion and microbody mediated by centrosomes(mitotic spindle pole bodies) in the primitive red alga Cyanidioschyzon merolae (2010)
  • Author(s): Imoto Y., Fujiwara T., Misumi O., Nishida K., Kuroiwa H. and Kuroiwa T.
  • Journal Title: Protoplasma Volume: 241 Pages: 3-74
  • Peer Reviewed
• [Journal Article] Review of cytological studies on cellular and molecular mechanisms of uniparental(maternal or paternal) inheritance of plastid and mitochondrial genomes induced by active digestion of organelle nuclei(nucleoids) (2010)
  • Author(s): Kuroiwa T.
  • Journal Title: J Plant Res Volume: 123 Pages: 207-230
  • Peer Reviewed
• [Journal Article] 100 years since discovery of non-Menderian plastid phenotypes (2010)
  • Author(s): Kuroiwa T
  • Journal Title: J Plant Res Volume: 123 Pages: 125-129
  • Peer Reviewed
• [Journal Article] VLIG is essential for the vacuole inheritance in Cyanidioschyzon merolae (2010)
  • Author(s): Fujiwara, T., Yagisawa, F., Misumi, O., Tashiro, K., Nishida, K., Yoshida, Y., Yoshida, M, Mori, T., Kuroiwa, H., and Kuroiwa T.
  • Journal Title: Plant Cell Volume: 22 Pages: 772-781
  • Peer Reviewed
• [Journal Article] New protein Pmn34 with an exonuclease motif localizes in the mitochondrial nucleoid periphery of Physarum polycephalum (2009)
  • Author(s): Itoh K, Kawano S., Kuroiwa H., Kuroiwa T., Higashiyama T., and Sasaki N., Murakami-Murofushi K.
  • Journal Title: Cytologia Volume: 74 Pages: 401-407
  • Peer Reviewed
• [Presentation] 真核細胞の誕生機構をオルガネラの分裂装置のゲノム科学解析から読む (2012)
  • Author(s): 黒岩常祥
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 立教大学
  • Year and Date: 2012-03-11
• [Presentation] クラミドモナスの培養日数がミトコンドリアに与える影響 (2011)
  • Author(s): 青山洋昭、黒岩常祥、中村宗一
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 葉緑体分化に関わる色素体シグマ因子SIG6の機能解析 (2011)
  • Author(s): 廣岡俊亮、華岡光正、黒岩晴子、黒岩常祥、田中寛
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] シゾンの運動性を利用した自然(非無菌)純化・同調培養法の開発 (2011)
  • Author(s): 黒岩常祥、吉田大和、井元裕太、大沼みお、黒岩晴子
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 蛍光融合タンパク質を利用したパーキンサス色素体およびミトコンドリアの細胞学・生化学的解析 (2011)
  • Author(s): 松崎素道、増田功、黒岩晴子、黒岩常祥、野崎久義、北潔
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 原始紅藻シゾンにおけるマイクロボディ分裂制御遺伝子の解析 (2011)
  • Author(s): 井元裕太、吉田大和、大沼みお、藤原崇之、吉田昌樹、三角修己、八木沢芙美、廣岡俊亮、黒岩晴子、河野重行、黒岩常祥
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] オルガネラの分裂または分配に関わる新規遺伝子の探索と解析 (2011)
  • Author(s): 大沼みお、吉田大和、井元裕太、藤原崇之、黒岩常祥
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 被子植物ゼラニウムの受精前後における胚嚢の変動について (2011)
  • Author(s): 黒岩晴子、黒岩常祥
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 硫化水素泉から採取された新規原始紅藻類の特性について (2011)
  • Author(s): 三角修己、小林優介、中村真心、藤田亜希子、黒岩晴子、黒岩常祥
  • Organizer: 日本植物学会75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Book] 生物学辞典 (2011)
  • Author(s): 黒岩常祥
  • Total Pages: 1615
  • Publisher: 東京化学同人
• [Book] 現代生物学入門 (2011)
  • Author(s): 黒岩常祥、浅島誠、小原雄治編
  • Total Pages: 2140
• [Patent(Industrial Property Rights)] LC-MALDIで得られたデータの比較解析方法 (2012)
  • Inventor(s): 黒岩常祥,吉田昌樹
  • Industrial Property Rights Holder: 立教大学
  • Industrial Property Number: 特許、W02012/29900
  • Acquisition Date: 2012-03-08
• [Patent(Industrial Property Rights)] LC-MALDIで得られたデータの比較解析方法 (2011)
  • Inventor(s): 黒岩常祥,吉田昌樹
  • Industrial Property Rights Holder: 立教大学
  • Industrial Property Number: 特許、PCT/JP2011/069885
  • Filing Date: 2011-09-01