2011 Fiscal Year Final Research Report

Synthesis and Application of Binding-typed Inhibitor for Infection of Influenza Virus on a Base of New Conception

Research Project

Project/Area Number	22658035
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Applied biochemistry
Research Institution	Ishikawa Prefectural University
Principal Investigator	YAMAMOTO Kenji 石川県立大学, 生物資源環境学部, 教授 (70109049)
Co-Investigator(Kenkyū-buntansha)	ASHIDA Hisashi 京都大学, 大学院・生命科学研究科, 准教授 (40379087)
Project Period (FY)	2010 – 2011
Keywords	インフルエンザウイルス / 感染阻害剤 / シアロ糖鎖 / アルギン酸 / キトサン
Research Abstract	We have succeeded in simple syntheses of efficient binding-inhibitors for influenza virus, which are composed of sialyl saccharides of glycopeptides extracted from hen egg yolk. We modified alginate and chitosan with multiple sialyl saccharides using condensation reaction and reductive amination reaction, respectively. These binding-inhibitors utilize the carbohydrate recognition of influenza virus for sialic acid, thus they can prevent the virus from infection. The resulting inhibitors showed sufficient inhibitory activity against influenza virus infection in MDCK cells. Unlike the other binding-inhibitors of influenza virus, these virus inhibitors require simple step in their syntheses.