Steroid hormones and their receptor interaction by Raman spectrometer analysis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KAWATA Mitsuhiro 京都府立医科大学, 医学研究科, 教授 (60112512)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Kenichi 京都府立医科大学, 医学研究科, 准教授 (40315932) ; TSUJIMURA Atsushi 京都府立医科大学, 医学研究科, 助教 (50236890)
• Co-Investigator(Renkei-kenkyūsha): KAKU Natsuko 堀場製作所,大阪大学, 免疫フロンティアセンター, 元研究員,元特任助教 ; MORISAKI Shinsuke 京都府立医科大学, 医学研究科, 助教 (20627294)
• Project Period (FY): 2010 – 2011
• Keywords: 細胞機能形態学

Research Abstract

We tried to investigate the interaction of steroid hormones and their receptor by Raman spectrometer analysis and challenged the possibility to visualize the discrimination of axon and myelin sheath of the sciatic nerve by this method. We got specific spectrum of estradiol and testosterone, but dilution protocol to apply for the in vitro study remains to be settled. Appropriate condition by Raman analysis to distinguish the axon and myelin was identified.

Research Products

• [Journal Article] Immunohistochemical analysis of the effects of estrogen on intraarticular neurogenic inflammation in a rat anterior cruciate ligament transection model of osteoarthritis (2012)
  • Author(s): Yoshida A, Morihara T, Matsuda K I, Sakamoto H, Arai Y, Kida Y, Kawata M, and Kubo T
  • Journal Title: Connect Tissue Res Volume: 53 Pages: 197-206
  • DOI: doi:10.3109/03008207.2011.628059
  • Peer Reviewed
• [Journal Article] Positive effect on bone fusion by the combination of platelet-rich plasma and a gelatin beta-tricalcium phosphate sponge : a study using a posterolateral fusion model of lumbar vertebrae in rats (2012)
  • Author(s): Okamoto S I, Ikeda T, Sawamura K, Nagae M, Hase H, Mikami Y, Tabata Y, Matsuda K I, Kawata M, and Kubo T
  • Journal Title: Tissue Eng Part A Volume: 18 Pages: 157-66
  • DOI: doi:10.1089/ten.tea.2011.0283
  • Peer Reviewed
• [Journal Article] Rapid signaling of steroid hormones in the vertebrate nervous system (2012)
  • Author(s): Sakamoto H, Takahashi H, Matsuda K I, Nishi M, Takanami K, Ogoshi M, Sakamoto T, Kawata M
  • Journal Title: Frontiers in Bioscience Volume: 17 Pages: 996-1019
  • DOI: doi:10.2741/3970
  • Peer Reviewed
• [Journal Article] Histone deacetylation during brain development is essential for permanent masculinization of sexual behavior (2011)
  • Author(s): Matsuda K I, Mori H, Nugent B M, Pfaff D W, McCarthy M M, and Kawata M
  • Journal Title: Endocrinology Volume: 152 Pages: 2760-2767
  • DOI: doi:10.1210/en2011.0193
  • Peer Reviewed
• [Journal Article] Highly visible expression of an oxytocin-monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh A, Fujihara H, Ohbuchi T, Onaka T, Hashimoto T, Kawata M, Suzuki H, Ueta Y
  • Journal Title: Endocrinology Volume: 152 Pages: 2768-2774
  • DOI: doi:10.1210/en.2011.0006
  • Peer Reviewed
• [Journal Article] Intranuclear dynamics of corticosteroid receptors and effects of proteasomal activity in cultured hippocampal neural cells (2011)
  • Author(s): Nishi M, Noriko H H, and Kawata M
  • Journal Title: Neurosci Lett Volume: 494 Pages: 65-69
  • DOI: doi:10.1016/j.neulet.2011.02.058
  • Peer Reviewed
• [Journal Article] In vivo assessment of peripheral nerve regeneration by diffusion tensor imaging (2011)
  • Author(s): Morisaki S, Kawai Y, Umeda M, Nishi M, Oda R, Fujiwara H, Yamada K, Higuchi T, Tanaka C, Kawata M, and Kubo T
  • Journal Title: J Magn Reson Imaging Volume: 33 Pages: 535-542
  • DOI: doi:10.1002/jmri.22442
  • Peer Reviewed
• [Journal Article] Intrauterine position affects estrogen receptor alpha expression in the ventromedial nucleus of the hypothalamus v ia promoter DNA methylation (2010)
  • Author(s): Mori H, Matsuda K, Tsukahara S, Kawata M
  • Journal Title: Endocrinology Volume: 151 Pages: 5775-5781
  • DOI: doi:10.1210/en.2010-0646
  • Peer Reviewed
• [Journal Article] Endogenous glucocorticoids improve myelination via Schwann cells after peripheral nerve injury : An in vivo study using a crush injury model (2010)
  • Author(s): Morisaki S, Nishi M, Fujiwara H, Oda R, Kawata M, and Kubo T
  • Journal Title: Glia Volume: 58 Pages: 954-963
  • DOI: doi:10.1002/glia.20977
  • Peer Reviewed
• [Journal Article] Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus (2010)
  • Author(s): Todoroki M, Ueta Y, Fujihara H, Otsubo H, Shibata M, Hashimoto H, Kobayashi M, Sakamoto H, Kawata M, Dayanithi G, Murphy D, Hiro H, Takahashi K, and Nagata S
  • Journal Title: Stress Volume: 13 Pages: 281-291
  • DOI: doi:10.3109/10253890903383406
  • Peer Reviewed
• [Presentation] 性ホルモン作用と受容体、行動制御の分子メカニズム (2011)
  • Author(s): 河田光博
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] アンドロゲン受容体の細胞内動態と性機能への関与 (2011)
  • Author(s): 河田光博
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都
  • Year and Date: 20110527-29
• [Presentation] 転写共役因子SAFB2による、エストロゲン受容体の動態と機能抑制 (2011)
  • Author(s): 橋本隆, 松田賢一, 河田光博
  • Organizer: 第1回4大学連携研究フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2011-12-09
• [Presentation] Sexual Neuroendocrinology : Sex Steroid Hormone Receptor Dynamism, Distribution and Functional Significance (2010)
  • Author(s): Kawata M
  • Organizer: IBRO School of Neuroscience
  • Place of Presentation: Selangor Darul Ehsan, Malaysia
  • Year and Date: 20101003-15
• [Presentation] アンドロゲン受容体の細胞内動態 (2010)
  • Author(s): 河田光博
  • Organizer: 第1回テストステロン研究会(第10回日本Men's Health医学会)
  • Place of Presentation: 東京
  • Year and Date: 2010-11-28
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/anat1/index.html