2011 Fiscal Year Final Research Report
Visualizing gene expression in response to environmental stimuli :real-time bioluminescence imaging in freely moving mice
| Project/Area Number |
22659047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
HONMA Sato 北海道大学, 大学院・医学研究科, 特任教授 (20142713)
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| Co-Investigator(Renkei-kenkyūsha) |
HAMADA Toshiyuki 北海道大学, 大学院・医学研究科, 特任講師 (20360208)
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| Project Period (FY) |
2010 – 2011
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| Keywords | イメージング / 時計遺伝子 / 生体リズム / 生物発光 / 転写調節 |
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| Research Abstract |
We aimed to develop a real-time monitoring system for reporter gene expression from the body surface of freely moving mice which can respond to various environmental stimuli. Using 2 CCD cameras, we performed stereoimaging to identify the region in a space and calibrated the luminescence in real-time. We tracked the region of interest by using 3 markers surrounding the region. By using this system and programs, we succeeded in demonstrating Per1-luc and Bmal1-luc expression from the olfactory bulb, cerebral cortex and skin simultaneously from freely moving mice at real time for more than 3 consecutive days. The system we developed enables us to monitor gene expression not only in single animal but to various social signals.
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[Journal Article] JNK regulates the photic response of the mammalian circadian clock2012
Author(s)
Yoshitane H, Honma S, Imamura K, Nakajima H, Nishide S, Ono D, Kiyota H, Shinozaki N, Matsuki H, Wada N, Doi H, Hamada T, Honma K and Fukada Y
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Journal Title
EMBO Report
Volume: 13
Pages: 455-561
DOI
Peer Reviewed
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