Highly sensitive diagnosis for virus based on next generation of hybridoma technology

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659115
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Mie University
• Principal Investigator: TOMITA Masahiro 三重大学, 大学院・工学研究科, 教授 (20183494)
• Research Collaborator: AKACHI Shigehiro
• Project Period (FY): 2010 – 2011
• Keywords: インフルエンザウイルス / 電気パルス融合 / ハイブリドーマテクノロジー / ビオチン/アビジン架橋 / モノクローナル抗体 / B細胞選択 / ELISA 法 / HI 試験法

Research Abstract

Three different types of influenza virus such as A/California/07/2009(H1N1), A/Brisbane/59/2007(H1N1) and A/Uruguay/716/2007(H3N2) were employed for immunization. As a result, each anti-serum indicated specificity to its own type of influenza virus by hemagglutination inhibition (HI) test. Selective production of monoclonal antibodies was carried out by use of next generation of hybridoma technology and some promising hybridoma cells secreting aimed monoclonal antibodies were obtained.

Research Products

• [Journal Article] Characterization of silica particles prepared via urease-catalyzed urea hydrolysis and activity of urease in sol-gel silica matrix (2012)
  • Author(s): Katsuya Kato, Masakazu Nishida, Kimiyasu Ito, Masahiro Tomita
  • Journal Title: pplied Surface Science Volume: (Accepted)
  • Peer Reviewed
• [Journal Article] Binding activity of avidin to the biotin within mesoporous silica materials for bioanalytical applications (2012)
  • Author(s): Toru Orita, Masahiro Tomita, Megumi Harada, Katsuya Kato
  • Journal Title: Analytical Biochemistry Volume: 425 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Immobilization of cholesterol esterase in mesoporous silica materials and its hydrolytic activity toward diethyl phthalate (2012)
  • Author(s): Toru Orita, Masahiro Tomita, Takao Saito, Masakazu Nishida, Katsuya Kato
  • Journal Title: aterials Science and Engineering C Volume: 32 Pages: 718-724
  • Peer Reviewed
• [Journal Article] Monitoring of membrane collapse and enzymatic reaction with single giant liposomes embedded in agarose gel (2011)
  • Author(s): Kanta Tsumoto, Masahiro Oohashi, Masahiro Tomita
  • Journal Title: Colloid and Polymer Science Volume: 289 Pages: 1337-1346
  • Peer Reviewed
• [Journal Article] Giant liposomes as microcapsules with large trapping volumes : Downsizing through various membrane filters and analysis with a calcein quenching method (2011)
  • Author(s): Kanta Tsumoto, Yuki Nakamura, Mina Yamazoe, Masahiro Tomita
  • Journal Title: Micro-Nano Mechatronics and Human Science, MHS 2011 Micro-Nano Global COE Pages: 439-444
  • Peer Reviewed
• [Journal Article] Hybridoma technologies for antibody production (2011)
  • Author(s): Masahiro Tomita, Kanta Tsumoto
  • Journal Title: Immunotherapy Volume: 3 Pages: 371-380
  • Peer Reviewed
• [Journal Article] Immobilization of antibodies on mesoporous silica materials and their binding abilities to antigens (2011)
  • Author(s): Toru Orita, Katsuya Kato, Masahiro Tomita
  • Journal Title: Journal of the Ceramic Society of Japan Volume: 119 Pages: 238-245
  • Peer Reviewed
• [Journal Article] Regulation of cellular responses to macroporous inorganic films prepared by the inverse-opal method (2011)
  • Author(s): Toru Orita, Masahiro Tomita, Katsuya Kato
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 84 Pages: 187-197
  • Peer Reviewed
• [Journal Article] New hybridoma technology based on antigen-specific immunoglobulin receptors (2011)
  • Author(s): Masahiro Tomita, Kanta Tsumoto
  • Journal Title: FEBS Journal Volume: Suppl. 1 Pages: 50
• [Presentation] 次世代ハイブリドーマテクノロジーの開発と応用 (2012)
  • Author(s): 冨田昌弘
  • Organizer: 第11回名古屋駅前イノベーションハブ技術シーズ発表会
  • Place of Presentation: 名古屋駅前イノベーションハブ(招待講演)
  • Year and Date: 2012-03-08
• [Presentation] 次世代ハイブリドーマテクノロジーの開発 (2011)
  • Author(s): 冨田昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] elective affinity purification of human IgG by a recombinant human FcγRI (2011)
  • Author(s): Yoshiharu Asaoka
  • Organizer: International Symposium for Sustainability by Engineering at MIU (IS^2 EMU 2011)
  • Place of Presentation: 三重大学
  • Year and Date: 2011-12-02
• [Presentation] Giant liposomes as microcapsules with large trapping volumes: Downsizing through various membrane filters and analysis with a calcein quenching method (2011)
  • Author(s): Kanta Tsumoto
  • Organizer: IEEE 2011 International Symposium on Micro-Nano Mechatronics and Human Science, MHS 2011
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-11-08
• [Presentation] New hybridoma technologies for producing monoclonal antibodies (2011)
  • Author(s): Masahiro Tomita
  • Organizer: The 16th International Conference on Human Antibodies & Hybridomas
  • Place of Presentation: Cannes, France
  • Year and Date: 2011-11-08
• [Presentation] マルチターゲッティングモノクローナル抗体作製技術の開発 (2011)
  • Author(s): 小畑晴香
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] 医療に応用可能な受容体タンパク質の立体構造特異的モノクローナル抗体作製技術の確立 (2011)
  • Author(s): 織田康行
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] 誘電泳動による粒子配列パタ ーンの拡張と細胞表面抗原検出への応用 (2011)
  • Author(s): 安川智之
  • Organizer: 日本分析化学会第 60 年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-16
• [Presentation] 海洋微量物質の同時検出を目指した連続的モノクローナル抗体作製技術の開発 (2011)
  • Author(s): 冨田昌弘
  • Organizer: 第14回マリンバイオテクノロジー学会大会
  • Place of Presentation: 静岡県コンベンションアーツセンター
  • Year and Date: 2011-05-28
• [Presentation] 受容体タンパク質の立体構造特異的モノクローナル抗体作製技術の開発 (2011)
  • Author(s): 織田康行
  • Organizer: 第75回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2011-05-28
• [Presentation] DNA免疫法に基づく立体構造認識モノクローナル抗体の選択的作製法の開発 (2010)
  • Author(s): 大嶋利征
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] ハプテン抗原特異的高性能モノクローナル抗体作製技術の開発 (2010)
  • Author(s): 早川智也
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] New hybridoma technology based on antigen-specific immunoglobulin receptors (2010)
  • Author(s): Masahiro Tomita
  • Organizer: 35th FEBS Congress
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2010-06-30
• [Presentation] ペプチド抗原に対する特異的モノクローナル抗体作製技術の開発 (2010)
  • Author(s): 小畑晴香
  • Organizer: 第74回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大学医学部
  • Year and Date: 2010-05-29
• [Presentation] The next generation of hybridoma technology (2010)
  • Author(s): Masahiro Tomita
  • Organizer: The 15th International Conference on Human Antibodies & Hybridomas
  • Place of Presentation: Porto, Portugal
  • Year and Date: 2010-04-16
• [Remarks] 冨田昌弘、東ソー(株):抗体医薬 効率的な作製法開発. 産経新聞 掲載日2011年2月9日
• [Remarks] 冨田昌弘、東ソー(株):がん治療薬開発に期待. 読売新聞 ONLINE 掲載日2011年2月4日
• [Remarks] 冨田昌弘、東ソー(株):「抗体医薬」作製法で特許. 読売新聞 掲載日 2011年2月 2日
• [Remarks] 冨田昌弘、東ソー(株):抗体医薬の作製法開発.がん治療の副作用軽減.伊勢新聞 掲載日 2011年2月2日
• [Patent(Industrial Property Rights)] 微生物の迅速診断を可能とする特異抗体の高効率作製法 (2013)
  • Inventor(s): 冨田昌弘
  • Industrial Property Rights Holder: 国立大学法人三重大学
  • Industrial Property Number: 特許, 特願 2013-209093(特願 2012-222032の国内優先権主張出願)
  • Filing Date: 2013-10-04
• [Patent(Industrial Property Rights)] 細胞膜タンパク質の立体構造を認識する抗体を産生するハイブリドーマの作製方法 (2010)
  • Inventor(s): 冨田昌弘、松葉隆雄
  • Industrial Property Rights Holder: 株式会社三重ティーエルオー、東ソー株式会社
  • Industrial Property Number: 特許, 特許第4599527号
  • Filing Date: 2010-10-08