Development of asbestos related tumor marker using protein array methods

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659124
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kochi University
• Principal Investigator: SUGANUMA Narufumi 高知大学, 教育研究部・医療学系, 教授 (50313747)
• Co-Investigator(Kenkyū-buntansha): HINO Okio 順天堂大学, 医学部, 教授 (90127910) ; KONDO Tadashi 国立がんセンター研究所, プロテオミクスバイオインフォマティクス, プロジェクトリーダー (30284061) ; HIROTA Ryoji 高知大学, 教育研究部・医療学系, 助教 (20448385) ; EITOKU Masamitsu 高知大学, 教育研究部・医療学系, 助教 (50552733)
• Project Period (FY): 2010 – 2011
• Keywords: 産業衛生 / プロテオミクス

Research Abstract

Protein array method was applied to develop biomarker measurement system. Manual arrayer was used to spot human plasma sample and HRP labeled anti-mesothelin antibody was applied. The system detects the protein at C terminal, while the available ELISA uses two antibodies for N and C terminals. The results from the two methods did not correlate each other. Further analysis using other clinical data including radiological findings is now underway. Optimization of protein array measurement to reduce processing time is also needed.

Research Products

• [Journal Article] Antibody-based protein profiling of the human chromosome 21 (2012)
  • Author(s): Uhlen, M., Oksvold, P., Algenas, C., Hamsten, C., Kondo, T., et al.
  • Journal Title: Mol Cell Proteomics Volume: 11 Pages: M111. 013458
  • Peer Reviewed
• [Journal Article] Macrophage-capping protein as a tissue biomarker for prediction of response to gemcitabine treatment and prognosis in cholangiocarcinoma (2012)
  • Author(s): Morofuji, N., Ojima, H., Onaya, H., Okusaka, T., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 75 Pages: 1577-1589
  • Peer Reviewed
• [Journal Article] Clinical proteomics identified ATP-dependent RNA helicase DDX39 as a novel biomarker to predict poor prognosis of patients with gastrointestinal stromal tumor (2012)
  • Author(s): Kikuta, K., Kubota, D., Saito, T., Orita, H., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 75 Pages: 1089-1098
  • Peer Reviewed
• [Journal Article] Proteomic study of malignant pleural mesothelioma by laser microdissection and two-dimensional difference gel electrophoresis identified cathepsin D as a novel candidate for a differential diagnosis biomarker (2012)
  • Author(s): Hosako, M., Muto, T., Nakamura, Y., Tsuta, K., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 75 Pages: 833-844
  • Peer Reviewed
• [Journal Article] Proteomic study identified HSP 70 kDa protein 1A as a possible therapeutic target, in combination with histone deacetylase inhibitors, for lymphoid neoplasms (2012)
  • Author(s): Fujii, K., Suzuki, N., Ikeda, K., Hamada, T., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 75 Pages: 1401-1410
  • Peer Reviewed
• [Journal Article] Preoperative nodal staging of uterine cancer : is contrast-enhanced PET/ CT more accurate than non-enhanced PET/ CT or enhanced CT alone? (2011)
  • Author(s): Kitajima K, Suzuki K, Senda M, Kita M, Nakamoto Y, Sakamoto S, Onishi Y, Maeda T, Yoshikawa T, Ohno Y, Suganuma N, Sugimura K
  • Journal Title: Ann Nucl Med Volume: 25 Pages: 5110
  • Peer Reviewed
• [Journal Article] Practice of universal precautions and risk of occupational blood-borne viral infection among Congolese health care workers (2011)
  • Author(s): Ngatu NR, Phillips EK, Wembonyama SO, Hirota R, Kaunge NJ, Mbutshu LH, Perry J, Yoshikawa T, Jagger J, Suganuma N
  • Journal Title: American Journal of Infection Control Volume: 01 Pages: 021
  • DOI: doi:10.1016/j.ajic
  • Peer Reviewed
• [Journal Article] Goishi Tea Consumption Inhibits Airway Hyperresponsiveness in BALB/ c Mice (2011)
  • Author(s): Hirota R, Ngatu NR, Miyamura M, Nakamura H, Suganuma N
  • Journal Title: BMC Immunology Volume: 12 Pages: 45
  • DOI: doi:10.1186/1471-2172-12-45
  • Peer Reviewed
• [Journal Article] Work form affects maximum oxygen uptake for one year in workers (2011)
  • Author(s): Hirai T, Kusaka Y, Suganuma N, Seo A, Tobita Y
  • Journal Title: Ind Health Volume: 49 Pages: 3210
  • Peer Reviewed
• [Journal Article] Secernin-1 as a novel prognostic biomarker candidate of synovial sarcoma revealed by proteomics (2011)
  • Author(s): Suehara, Y., Tochigi, N., Kubota, D., Kikuta, K., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 74 Pages: 829-842
  • Peer Reviewed
• [Journal Article] Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis (2011)
  • Author(s): Muto, T., Taniguchi, H., Kushima, R., Tsuda, H., Kondo, T., et al.
  • Journal Title: J Proteomics Volume: 74 Pages: 858-873
  • Peer Reviewed
• [Journal Article] Proteomics-based disease biomarkers (2011)
  • Author(s): Misek, D. E., Kondo, T., Duncan, M. W.
  • Journal Title: Int J Proteomics Volume: 2011 Pages: 894618
  • Peer Reviewed
• [Journal Article] Steroid receptor expression in thymomas and thymic carcinomas (2011)
  • Author(s): Mimae, T., Tsuta, K., Takahashi, F., Yoshida, A., Kondo, T., et al.
  • Journal Title: Cancer Volume: 117 Pages: 4396-4405
  • Peer Reviewed
• [Journal Article] Pfetin as a prognostic biomarker for gastrointestinal stromal tumor : validation study in multiple clinical facilities (2011)
  • Author(s): Kubota, D., Orita, H., Yoshida, A., Gotoh, M., Kondo, T., et al.
  • Journal Title: Jpn J Clin Oncol Volume: 41 Pages: 1194-1202
  • Peer Reviewed
• [Journal Article] Developments for a growing Japanese patient population : facilitating new technologies for future health care (2011)
  • Author(s): Kato, H., Nishimura, T., Ikeda, N., Yamada, T., Kondo, T., et al.
  • Journal Title: Proteomics Volume: 74 Pages: 759-764
  • Peer Reviewed
• [Journal Article] Combined use of a solid-phase hexapeptide ligand library with liquid chromatography and two-dimensional difference gel electrophoresis for intact plasma proteomics (2011)
  • Author(s): Hagiwara, T., Saito, Y., Nakamura, Y., Tomonaga, T., Kondo, T., et al.
  • Journal Title: Int J Proteomics Volume: 2011 Pages: 739615
  • Peer Reviewed
• [Journal Article] Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification (2011)
  • Author(s): Gotoh, M., Arai, E., Wakai-Ushijima, S., Hiraoka, N., Kondo, T., et al.
  • Journal Title: J Biomed Biotechnol Volume: 2011 Pages: 780836
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues (2011)
  • Author(s): Arai, E., Wakai-Ushijima, S., Fujimoto, H., Hosoda, F., Kondo, T., et al.
  • Journal Title: Pathobiology Volume: 78 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effects of limonene from yuzu(Citrus junos Tanaka) essential oil on eosinophils (2010)
  • Author(s): Hirota R, Roger NN, Nakamura H, Song HS, Sawamura M, Suganuma N
  • Journal Title: J Food Sci Volume: 75(3) Pages: H87-92
  • Peer Reviewed
• [Journal Article] Effect of a two-hour training on physicians' skill in interpreting Pneumoconiotic chest radiographs (2010)
  • Author(s): Ngatu NR, Suzuki S, Kusaka Y, Shida H, Akira M, Suganuma N
  • Journal Title: J Occup Health Volume: 52(5) Pages: 294-301
  • Peer Reviewed
• [Journal Article] Structure of the histone chaperone CIA/ ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction (2010)
  • Author(s): Akai Y, Adachi N, Hayashi Y, Eitoku M, Sano N, Natsume R, Kudo N, Tanokura M, Senda T, Horikoshi M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(18) Pages: 8153-8
  • Peer Reviewed
• [Journal Article] DEK oncoprotein regulates transcriptional modifiers and sustains tumor initiation activity in high-grade neuroendocrine carcinoma of the lung (2010)
  • Author(s): Shibata, T., Kokubu, A., Miyamoto, M., Hosoda, F., Kondo, T., et al.
  • Journal Title: Oncogene Volume: 29 Pages: 4671-4681
  • Peer Reviewed
• [Journal Article] Establishment of six new human biliary tract carcinoma cell lines and identification of MAGEH1 as a candidate biomarker for predicting the efficacy of gemcitabine treatment (2010)
  • Author(s): Ojima, H., Yoshikawa, D., Ino, Y., Shimizu, H., Kondo, T., et al.
  • Journal Title: Cancer Sci Volume: 101 Pages: 882-888
  • Peer Reviewed
• [Journal Article] Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomics (2010)
  • Author(s): Kikuta, K., Tochigi, N., Saito, S., Shimoda, T., Kondo, T., et al.
  • Journal Title: Proteomics Clin Appl Volume: 4 Pages: 560-567
  • Peer Reviewed
• [Journal Article] Significance of PGP9. 5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinoma (2010)
  • Author(s): Akishima-Fukasawa, Y., Ino, Y., Nakanishi, Y., Miura, A., Kondo, T., et al.
  • Journal Title: Am J Clin Pathol Volume: 134 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Perception of the risk of sexual transmission of HIV among Congolese and Japanese university students
  • Author(s): Ngatu NR, Hirota R, Eitoku M, Muzembo BA, Nishimori M, Kuramochi M, Shintani S, Inoue S, Takiuchi R, Maegawa M, Ribble D, Mbenza MA, Situakibanza NTH, Mbanzulu PD, Suganuma N
  • Journal Title: Environmental Health and Preventive Medicine
  • DOI: DOI:10.1007/s12199-011-0232-z
  • Peer Reviewed
• [Presentation] Proteomics application for sarcoma : The symposium of therapy and diagnosis of bone and soft tissue neoplasm in upper extremity (2012)
  • Author(s): 近藤格
  • Organizer: 手外科研究所
  • Place of Presentation: 上海、中国
  • Year and Date: 20120000
• [Presentation] プロテオーム解析による個別化医療のためのバイオマーカー解析 (2012)
  • Author(s): 近藤格
  • Organizer: 日本臨床検査学会
  • Place of Presentation: 岡山
  • Year and Date: 20120000
• [Presentation] A linkage application of multi-dimensional chromatography, solid-phase peptide ligand library, two-dimensional difference gel electrophoresis and mass spectrometry towards plasma biomarker development (2011)
  • Author(s): 近藤格
  • Organizer: HPLC研究会
  • Place of Presentation: ブタペスト・ハンガリー
  • Year and Date: 20110000
• [Presentation] プロテオーム解析によるがんバイオマーカー解析(シンポジウム) (2011)
  • Organizer: 日本プロテオーム学会
  • Place of Presentation: 新潟
  • Year and Date: 20110000
• [Presentation] SOLID-PHASE HEXAPEPTIDE LIGAND LIBRARY WITH LIQUID CHROMATOGRAPHY AND TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS FOR INTACT PLASMA PROTEOMICS (2011)
  • Author(s): 近藤格
  • Organizer: ヒトプロテオーム機構年会
  • Place of Presentation: ジュネーブ、スイス
  • Year and Date: 20110000
• [Presentation] Cancer Proteomics for Biomarker Development toward Personalized Medicine (2011)
  • Author(s): 近藤格
  • Organizer: 東・中央ヨーロッパプロテオーム学会
  • Place of Presentation: プラハ、チェコ
  • Year and Date: 20110000
• [Presentation] Proteomic Study of Malignant Pleural Mesothelioma by Laser Microdissection and Two-dimensional Difference Gel Electrophoresis : Cathepsin D as a Novel Candidate for a Differential Diagnosis Biomarker (2011)
  • Author(s): 近藤格
  • Organizer: 結合組織腫瘍学会
  • Place of Presentation: シカゴ、米国
  • Year and Date: 20110000
• [Presentation] Cancer Proteomics for Biomarker Development toward Personalized Medicine (2011)
  • Author(s): 近藤格
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20110000
• [Presentation] Cancer Proteomics for Biomarker Development toward Personalized Medicine (2011)
  • Author(s): 近藤格
  • Organizer: 日米癌学会合同会議
  • Place of Presentation: 千葉
  • Year and Date: 20110000
• [Presentation] Asbestos exposure among Primary Lung Cancer Patients in eastern Japan (2010)
  • Author(s): Suganuma N, Tamura A, Funakoshi M, et al
  • Organizer: American Thoracic Society
  • Place of Presentation: New Orleans
  • Year and Date: 20100514-19
• [Presentation] Cancer Proteomics for Biomarker Development (2010)
  • Author(s): 近藤格
  • Organizer: 日本プロテオーム学会シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 20100000
• [Presentation] 蛍光二次元電気泳動法と抗体を用いたプロテオーム解析による大腸がんバイオマーカーの開発 (2010)
  • Author(s): 近藤格
  • Organizer: 大腸癌研究会
  • Place of Presentation: 鹿児島
  • Year and Date: 20100000
• [Presentation] 電気泳動法を用いたがん個別化医療のためのバイオマーカー開発 (2010)
  • Author(s): 近藤格
  • Organizer: 日本電気泳動学会
  • Place of Presentation: 札幌
  • Year and Date: 20100000
• [Presentation] Cancer Proteomics for Biomarker Development toward Personalized Medicine (2010)
  • Author(s): 近藤格
  • Organizer: 日本癌学会学術集会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 20100000
• [Presentation] Cancer Proteomics for Biomarker Development (2010)
  • Author(s): 近藤格
  • Organizer: ヨーロッパプロテオーム学会、エストリル
  • Place of Presentation: ポルトガル
  • Year and Date: 20100000
• [Presentation] Biomarker Development by Sarcoma Proteomics (2010)
  • Author(s): 近藤格
  • Organizer: 結合組織腫瘍学会
  • Place of Presentation: パリ、フランス
  • Year and Date: 20100000
• [Presentation] Cancer Proteomics for Biomarker Development toward Personalized Medicine (2010)
  • Author(s): 近藤格
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 20100000