Elucidation of the radio-sensitization mechanism by heat

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659224
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: TAKAHASHI Akihisa 群馬大学, 先端科学研究指導者育成ユニッ ト, 准教授 (60275336)
• Project Period (FY): 2010 – 2012
• Keywords: 放射線治療 / 温熱治療 / 感受性 / 放射線増感 / DNA二本鎖切断

Research Abstract

We already proposed the formation of DNA double-strand breaks (DSBs) by heat-treatment. Here we found that the HR-deficient cells were sensitive, and were low efficiency of DSB repair by heat when compared to the NHEJ-deficient cells and their parental cells. In addition, we demonstrated that heat-induced DSBs were repaired by HR. Our novel findings suggest that the HR exerts an importantrole in the radio-sensitization mechanism by heat.

Research Products

• [Journal Article] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells (2013)
  • Author(s): Watanabe K, Miyagawa R, Tomikawa C, Mizuno R, Takahashi A, Hori H, Ijiri K.
  • Journal Title: Nucleic Acids Res Volume: 41 Pages: 4671-4685
  • DOI: DOI:10.1093/nar/gkt153
  • Peer Reviewed
• [Journal Article] Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells (2012)
  • Author(s): Shimura T, Noma N, Oikawa T, Ochiai Y, Kakuda S, Kuwahara Y, Takai Y, TakahashiA, Fukumoto M.
  • Journal Title: Oncogenesis Volume: 1 Pages: e12
  • DOI: DOI:10.1038/ oncsis.2012.12
  • Peer Reviewed
• [Journal Article] DNA double-strand breaks induced by cavitational mechanical effects of ultrasound in cancer cell lines (2012)
  • Author(s): Furusawa Y, Fujiwara Y, Campbell P, Zhao Q-L, Ogawa R, Hassan MA, Tabuchi Y, Takasaki I, Takahashi A, Kondo T.
  • Journal Title: PLoS ONE Volume: 7 Pages: e29012
  • DOI: DOI:10.1371/journal.pone.0029012
  • Peer Reviewed
• [Journal Article] siRNA targeted for nbs1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Okamoto N, Takahashi A, Ota I, Ohnishi K, Mori E, Kondo N, Noda T, Nakagawa Y, Uemura H, Yane K, Hosoi H, Ohnishi T.
  • Journal Title: Int J Hyperthermia Volume: 27 Pages: 297-304
  • DOI: DOI:10.3109/02656736.2010.545365
  • Peer Reviewed
• [Journal Article] Effect of rapamycin, an mTOR inhibitor, on radiation sensitivity of lung cancer cells having different p53 gene status (2010)
  • Author(s): Nagata Y, Takahashi A, Ohnishi K, Ota I, Ohnishi T, Tojo T, Taniguchi S.
  • Journal Title: Int J Oncol Volume: 37 Pages: 1001-1010
  • DOI: DOI:10.3892/ijo_00000751
  • Peer Reviewed
• [Journal Article] ATM is the predominant kinase involved in the phosphorylation of histone H2AX after heating (2010)
  • Author(s): Takahashi A, Mori E, Su X, Nakagawa Y, Okamoto N, Uemura H, Kondo N, Noda T, Toki A, Ejima Y, Chen JD, Ohnishi K, Ohnishi T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 417-422
  • DOI: DOI:10.1269/jrr.10015
  • Peer Reviewed
• [Journal Article] The foci of DNA double strand break-recognition proteins localize with γH2AX after heat treatment (2010)
  • Author(s): Takahashi A, Mori E, Ohnishi T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 91-95
  • DOI: DOI:10.1269/jrr.09111
  • Peer Reviewed
• [Presentation] 放射線抵抗性がん幹細胞様細胞に対する温熱感受性 (2013)
  • Author(s): 高橋昭久,馬洪玉,久保誠,中川彰子,吉田由香里,桑原義和,福本学
  • Organizer: 第17回関東ハイパーサーミア研究会/全身ハイパーサーミア研究会合同学術研究会
  • Place of Presentation: 東京厚生年金病院(東京都)
  • Year and Date: 2013-03-02
• [Presentation] ATM is the predominant kinase involved in the phosphorylation of histone H2AX after heating (2012)
  • Author(s): Takahashi A, Mori E, Chen DJ, Ohnishi T
  • Organizer: 11th International Congress of Hyperthermic Oncology
  • Place of Presentation: ハイア ットリージェンシー京都 (京都府)
  • Year and Date: 2012-08-29
• [Presentation] Hyperthermicsensitization efficacy as a target of homologous recombination repair pathways in the oral cancer cells (2012)
  • Author(s): Kajihara A, Nakagawa Y, Mori E, TakahashiA, Kirita T, Ohnishi T.
  • Organizer: 11th International Congress of Hyperthermic Oncology
  • Place of Presentation: ハイアットリージェンシー京都 (京都府)
  • Year and Date: 2012-08-29
• [Presentation] A priming heat treatment can induce the development of heat- and radio-resistance via HSPs, regardless of p53-gene status (2012)
  • Author(s): Takahashi A, Ohnishi T.
  • Organizer: 11th International Congress of Hyperthermic Oncology
  • Place of Presentation: ハイアットリージェ ンシー京都 (京都府)
  • Year and Date: 2012-08-29
• [Presentation] 放射線抵抗性細胞における温熱感受性についての検討 (2012)
  • Author(s): 高橋昭久,馬洪玉,久保誠,中川彰子,吉田由香里,桑原義和,福本学
  • Organizer: 第18回癌治療増感研究シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2012-06-09
• [Presentation] 温熱感受性を決定する分子機構 (2012)
  • Author(s): 高橋昭久
  • Organizer: 第16回関東ハイパーサーミア研究会/全身ハイパーサーミア研究会合同学術研究会
  • Place of Presentation: 埼玉医科大学かわごえクリニック(埼玉県)
  • Year and Date: 2012-03-24
• [Presentation] Target genes deciding heat sensitivity (2011)
  • Author(s): Takahashi A.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] 温熱感受性に対するDNA二本鎖切断修復の役割について (2011)
  • Author(s): 高橋昭久
  • Organizer: 日本ハイパーサーミア学会第28回大会
  • Place of Presentation: ウインクあいち(愛知県)
  • Year and Date: 2011-09-10
• [Presentation] DNA修復を標的とした温熱増感研究 (2011)
  • Author(s): 高橋昭久,森英一朗,近藤夏子,野田太一,梶原淳久,長谷川正俊,大西武雄
  • Organizer: 第17回癌治療増感研究シンポジウム
  • Place of Presentation: 赤門鍼灸柔整専門学校(宮城県)
  • Year and Date: 2011-06-08
• [Remarks]
  • URL: http://asrldu.dept.med.gunma-u.ac.jp/atakahashi/akihisa_takahashi.html