Single Cell Tracking of Glioma Invasion using MRI Reporter Gene.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659259
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Osaka University
• Principal Investigator: ARITA Hideyuki 大阪大学, 大学院・医学系研究科, 招へい研究員 (60570570)
• Co-Investigator(Kenkyū-buntansha): KINOSHITA Manabu 大阪大学, 大学院・医学系研究科, 助教 (40448064) ; YOSHIOKA Yoshichika 大阪大学, 免疫学フロンティア研究センター, 特任教授 (00174897)
• Project Period (FY): 2010 – 2011
• Keywords: MRI / レポーター遺伝子 / 神経膠芽腫

Research Abstract

We have attempted to elucidate the mechanism of glioblastoma cell brain infiltration by use of an MRI reporter gene ; MagA. By overexpressing MagA in glioblastoma cells, it is possible to perform in vivo imaging of these cell by use of MRI. In this study, we were able to establish a MagA-overexpressing glioblastoma cell line, and those cells were successfully transplanted into the brain of a Balb/ c nude mouse. Tumor expansion was observed on MRI. These results suggest that MagA is a powerful MRI reporter gene that can be used for in vivo MR imaging.

Research Products

• [Journal Article] Cardiac-cycle related volume change in unruptured cerebral aneurysms : a detailed volume quantification study using 4DCTA (2012)
  • Author(s): Kuroda J, Kinoshita M, Tanaka H, Nishida T, Nakamura H, Watanabe Y, Tomiyama N, Fujinaka T, Yoshimine T
  • Journal Title: Stroke Volume: 43(1) Pages: 61-66
  • DOI: doi:10.1161/STROKEAHA.111.626846
  • Peer Reviewed
• [Journal Article] Use of(11) C-methionine PET parametric response map for monitoring WT1 immunotherapy response in recurrent malignant glioma (2012)
  • Author(s): Chiba Y, Kinoshita M, Okita Y, Tsuboi A, Isohashi K, Kagawa N, Fujimoto Y, Oji Y, Oka Y, Shimosegawa E, Morita S, Hatazawa J, Sugiyama H, Hashimoto N, Yoshimine T
  • Journal Title: J Neurosurg Volume: 116(4) Pages: 835-842
  • DOI: doi:10.3171/2011.12.JNS111255
  • Peer Reviewed
• [Journal Article] 11C-methionine uptake and intraoperative 5-aminolevulinic acid-induced fluorescence as separate index markers of cell density in glioma : a stereotactic image-histological analysis (2012)
  • Author(s): Arita H, Kinoshita M, Kagawa N, Fujimoto Y, Kishima H, Hashimoto N, Yoshimine T
  • Journal Title: Cancer Volume: 118(6) Pages: 1619-1627
  • DOI: doi:10.1002/cncr.26445
  • Peer Reviewed
• [Journal Article] Slower growth of skull base meningiomas compared with non-skull base meningiomas based on volumetric and biological studies (2012)
  • Author(s): Hashimoto N, Rabo CS, Okita Y, Kinoshita M, Kagawa N, Fujimoto Y, Morii E, Kishima H, Maruno M, Kato A, Yoshimine T
  • Journal Title: J Neurosurg Volume: 116(3) Pages: 574-580
  • DOI: doi:10.3171/2011.11.JNS11999
  • Peer Reviewed
• [Journal Article] Imaging 18F-fluorodeoxy glucose/ 11C-methionine uptake decoupling for identification of tumor cell infiltration in peritumoral brain edema (2011)
  • Author(s): Kinoshita M, Goto T, Arita H, Okita Y, Ishohashi K, Kagawa N, Fujimoto Y, Kishima H, Shimosegawa E, Saitoh Y, Hatazawa J, Hashimoto N, Yoshimine T
  • Journal Title: J Neuro-oncol Volume: 106(2) Pages: 417-425
  • DOI: doi:10.1007/s11060-011-0688-0
  • Peer Reviewed
• [Journal Article] Clinical characteristics of meningiomas assessed by 11C-methionine and 18F-fluorodeoxyglucose positron emission tomography (2011)
  • Author(s): Arita H, Kinoshita M, Okita Y, Hirayama R, Watabe T, Isohashi K, Kijima N, Kagawa N, Fujimoto Y, Kishima H, Shimosegawa E, Hatazawa J, Hashimoto N, Yoshimine T
  • Journal Title: J Neuro-oncol Volume: 107(2) Pages: 379-386
  • DOI: doi:10.1007/s11060-011-0759-2
  • Peer Reviewed
• [Journal Article] Hemifacial spasm caused by intra-axial brainstem cavernous angioma with venous angiomas (2011)
  • Author(s): Arita H, Kishima H, Hosomi K, Iwaisako K, Hashimoto N, Saitoh Y, Yoshimine T
  • Journal Title: Br J Neurosurg Volume: 26(2) Pages: 281-283
  • DOI: doi:10.3109/02688697.2011.609605
  • Peer Reviewed
• [Journal Article] CD166/ Activated leukocyte cell adhesion molecule(ALCAM) is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion (2011)
  • Author(s): Kijima N, Hosen N, Kagawa N, Hashimoto N, Nakano A, Fujimoto Y, Kinoshita M, Sugiyama H, Yoshimine T
  • Journal Title: Neuro-oncology
  • DOI: doi:10.1093/neuonc/nor202
  • Peer Reviewed
• [Journal Article] Hepatitis B virus reactivation associated with temozolomide for malignant glioma : a case report and recommendation for prophylaxis (2011)
  • Author(s): Fujimoto Y, Hashimoto N, Kinoshita M, Miyazaki Y, Tanaka S, Yakushijin T, Takehara T, Kagawa N, Yoshimine T
  • Journal Title: Int J Clin Oncol
  • DOI: doi:10.1007/s10147-011-0294-3
  • Peer Reviewed
• [Journal Article] Significance of Tumor Volume Related to Peritumoral Edema in Intracranial Meningioma Treated with Extreme Hypofractionated Stereotactic Radiation Therapy in Three to Five Fractions (2011)
  • Author(s): Morimoto M, Yoshioka Y, Shiomi H, Isohashi F, Konishi K, Kotsuma T, Fukuda S, Kagawa N, Kinoshita M, Hashimoto N, Yoshimine T, Koizumi M
  • Journal Title: Jpn J Clin Oncol Volume: 41(5) Pages: 609-616
  • DOI: doi:10.1093/jjco/hyr022
  • Peer Reviewed
• [Journal Article] Quantification of cerebral artery motion during the cardiac cycle (2011)
  • Author(s): Nishida T, Kinoshita M, Tanaka H, Fujinaka T, Yoshimine T
  • Journal Title: AJNR Am J Neuroradiol Volume: 32(11) Pages: E206-208
  • DOI: doi:10.3174/ajnr.A2354
  • Peer Reviewed
• [Journal Article] A survey of disclosure of diagnosis to patients with glioma in Japan (2011)
  • Author(s): Yamamoto F, Hashimoto N, Kagawa N, Okita Y, Chiba Y, Kijima N, Kinoshita M, Yoshizu K, Fujimoto Y, Hirai K, Yoshimine T
  • Journal Title: Int J Clin Oncol Volume: 16(3) Pages: 230-237
  • DOI: doi:10.1007/s10147-010-0152-8
  • Peer Reviewed
• [Journal Article] Posttransplant Lymphoproliferative Disease of the Central Nervous System After Kideny Transplantation : Single Center Experience Over 40 Years. Two Case Reports- (2010)
  • Author(s): Arita H, Izumoto S, Kinoshita M, Okita Y, Hashimoto N, Fujita T, Ichimaru N, Takahara S, Yoshimine T
  • Journal Title: Neurol Med Chir(Tokyo) Volume: 50(12) Pages: 1079-1083
  • URL: http://dx.doi.org/10.2176/nmc.50.1079
  • Peer Reviewed
• [Journal Article] Diffusion tensor based tumor infiltration index cannot discriminate vasogenic edema from tumor-infiltrated edema (2010)
  • Author(s): Kinoshita M, Goto T, Okita Y, Kagawa N, Kishima H, Hashimoto N, Yoshimine T
  • Journal Title: J Neuro-onco Volume: 96(3) Pages: 409-415
  • DOI: doi:10.1007/s11060-009-9979-0
  • Peer Reviewed
• [Journal Article] 11C-methionine uptake correlates with tumor cell density rather than with microvessel density in glioma : a stereotactic image-histology comparison (2010)
  • Author(s): Okita Y, Kinoshita M, Goto T, Kagawa N, Kishima H, Shimosegawa E, Hatazawa J, Hashimoto N, Yoshimine T
  • Journal Title: Neuroimage Volume: 49(4) Pages: 2977-2982
  • URL: http://dx.doi.org/10.1016/j.neuroimage
  • Peer Reviewed
• [Journal Article] A surgically treated case of Lhermitte-Duclos disease with a precise natural history and high uptake of FDG on PET (2010)
  • Author(s): Goto Y, Hashimoto N, Okita Y, Goto T, Rabo C, Hirayama H, Horikawa Y, Kinoshita M, Kagawa N, Yoshimine T
  • Journal Title: J Neuro-oncol Volume: 97(3) Pages: 445-450
  • DOI: doi:10.1007/s11060-009-0042-y
  • Peer Reviewed
• [Journal Article] MR molecular imaging of HER-2 in a murine tumor xenograft by SPIO labeling of anti-HER-2 affibody (2010)
  • Author(s): Kinoshita M, Yoshioka Y, Okita Y, Hashimoto N, Yoshimine T
  • Journal Title: Contrast Media Mol Imaging Volume: 5(1) Pages: 18-22
  • DOI: doi:10.1002/cmmi.363
  • Peer Reviewed
• [Journal Article] Effects of concomitant temozolomide and radiation therapies on WT1-specific T cells in malignant glioma (2010)
  • Author(s): Chiba Y, Hashimoto N, Tsuboi A, Oka Y, Maruo A, Kinoshita M, Kagawa N, Oji Y, Hosen N, Nishida S, Sugiyama H, Yoshimine T
  • Journal Title: Jpn J Clin Oncol Volume: 40(5) Pages: 395-403
  • DOI: doi:0.1093/jjco/hyp196
  • Peer Reviewed
• [Journal Article] Immunohistological profiling by B-cell differentiation status of primary central nervous system lymphoma treated by high-dose methotrexate chemotherapy (2010)
  • Author(s): Kinoshita M, Hashimoto N, Izumoto S, Okita Y, Kagawa N, Maruno M, Ohnishi T, Arita N, Yoshimine T
  • Journal Title: J Neuro-oncol Volume: 99(1) Pages: 95-101
  • DOI: doi:10.1007/s11060-010-0112-1
  • Peer Reviewed
• [Journal Article] Prognostic value of WT1 protein expression level and MIB-1 staining index as predictor of response to WT1 immunotherapy in glioblastoma patients (2010)
  • Author(s): Chiba Y, Hashimoto N, Tsuboi A, Rabo C, Oka Y, Kinoshita M, Kagawa N, Oji Y, Sugiyama H, Yoshimine T
  • Journal Title: Brain Tumor Pathol Volume: 27(2) Pages: 29-34
  • DOI: doi:10.1007/s10014-010-0265-9
  • Peer Reviewed
• [Presentation] クッシング病診断における静脈血サンプリング～診断基準における位置づけからの検討 (2012)
  • Author(s): 有田英之
  • Organizer: 第22回日本間脳下垂体腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-25
• [Presentation] IgG4関連下垂体炎における下垂体炎組織と周辺炎症性硬膜組織の違い (2012)
  • Author(s): 木下学
  • Organizer: 第22回日本間脳下垂体腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2012-02-24
• [Presentation] 非造影グリオーマ病変ではFDG/ 11C-methionine PET非同期度が腫瘍細胞密度を反映する (2011)
  • Author(s): 木下学
  • Organizer: 第29回日本脳腫瘍学会学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-11-28
• [Presentation] 神経膠腫手術においてアミノ酸PET画像誘導と5-ALA蛍光診断の併用は有用か? (2011)
  • Author(s): 有田英之
  • Organizer: 第29回日本脳腫瘍学会学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-11-27
• [Presentation] Voxel-wise analysis of FDG and 11C-methionine PET can accurately detect glioma infiltration in the MR T2-hyper-intense area (2011)
  • Author(s): Manabu Kinoshita
  • Place of Presentation: California, USA
  • Year and Date: 2011-11-18
• [Presentation] 11C-methionine uptake and intraoperative 5-aminolevulinic acid induced fluorescence are separate index markers of cell density in gliomas : a stereotactic image-histological analysis (2011)
  • Author(s): Hideyuki Arita
  • Place of Presentation: California, USA
  • Year and Date: 2011-11-18
• [Presentation] TRH負荷試験への反応性と腫瘍体積に関する検討 (2011)
  • Author(s): 有田英之
  • Place of Presentation: 東京
  • Year and Date: 2011-11-05
• [Presentation] FDG/ 11C-methionine PET非同期度解析はグリオーマの脳組織浸潤度の定量的評価を可能にする (2011)
  • Author(s): 木下学
  • Organizer: 第70回日本脳神経外科学会総会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-10-14
• [Presentation] アミノ酸PET画像と5-ALA術中傾向診断併用はグリオーマ手術に有用化? (2011)
  • Author(s): 有田英之
  • Organizer: 第70回日本脳神経外科学会総会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-10-13
• [Presentation] Segmentation of tumor infiltrative and vasogenic edema in brain tumors using voxel-wise analysis of 11C-methinonine and FDG PET and its comparison with diffusion tensor imaging (2011)
  • Author(s): Manabu Kinoshita
  • Organizer: International Society for Magnetic Resonance in Medicine(ISMRM) 19th Scientific Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-05-09
• [Presentation] PET画像誘導下神経膠腫手術における5-ALA術中蛍光診断の意義 (2011)
  • Author(s): 有田英之
  • Organizer: 第1回ボルフィリン-ALA学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-07
• [Presentation] 先端巨大症における術前TRH反応性と腫瘍体積の解析 (2011)
  • Author(s): 有田英之
  • Organizer: 第87回日本内分泌学会学術総会
  • Place of Presentation: 兵庫
  • Year and Date: 2011-04-21
• [Presentation] Cushing病における静脈血サンプリングの意義の検討 (2011)
  • Author(s): 有田英之
  • Organizer: 第21回日本間脳下垂体腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-12
• [Presentation] IgG4関連下垂体炎と下垂体を標的としない頭蓋内IgG4関連疾患の違い (2011)
  • Author(s): 木下学
  • Organizer: 第21回日本間脳下垂体腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-12
• [Presentation] B細胞分化過程からみた中枢神経系悪性リンパ腫 (2010)
  • Author(s): 木下学
  • Organizer: 第40回ニューロオンコロジーの会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-04
• [Presentation] T2高輝度領域でのglucose/ methionine代謝の非同期性を用いたグリオーマ浸潤の画像化 (2010)
  • Author(s): 木下学
  • Organizer: 第28回日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢
  • Year and Date: 2010-11-28
• [Presentation] Visualization and numerical quantification of cardiac cycle-related physiological motions of the cerebral arteries in humans (2010)
  • Author(s): Nishida Takeo
  • Organizer: Neuroscience 2010-40^<th> Annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-17
• [Presentation] Glucose and methionine uptake decouples in T2-hyperintese area with tumor infiltration but not in pure vasogenic edema (2010)
  • Author(s): Kinoshita Manabu
  • Organizer: Neuroscience 2010-40^<th> Annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] 多画像同時解析による新規画像診断法の開発: voxel-wise analysisが可能にする真のマルチモダール画像解析 (2010)
  • Author(s): 木下学
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-27
• [Presentation] Affibodyを用いたMRIによるHER-2受容体の分子イメージング (2010)
  • Author(s): 木下学
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] MR molecular imaging of HER-2 in a murine tumor xenograft by SPIO labeling of anti-HER-2 Affibody (2010)
  • Author(s): Kinoshita Manabu
  • Organizer: International Society for Magnetic Resonance in Medicine(ISMRM) 18th Scientific Meeting
  • Place of Presentation: Stockholm, Sweeden
  • Year and Date: 2010-05-03