2012 Fiscal Year Final Research Report
Establishment of rapid detection system for intra-amniotic infection and drug susceptibility test by PCR using eukaryote-made Taq polymerase
| Project/Area Number |
22659297
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Toyama |
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Principal Investigator |
SAITO Shigeru 富山大学, 大学院・医学薬学研究部(医学), 教授 (30175351)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 絨毛膜羊膜炎 / 早産 / 感染 / PCR / Ureaplasma |
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| Research Abstract |
We have established a novel method to detect bacterial, Mycoplasma, Ureaplasma and fungi in the amniotic fluid using eukaryote-made Taq polymerase. Using this system, we have evaluated the bacterial infection in amniotic fluid of 86 preterm labor cases. Surprisingly, we have detected double infection with bacterial Ureaplasma in 13 case, Ureaplasma infection cases in 5 cases, bacterial infection cases in 13 cases, and infection rate in preterm labor cases accounted for 37.2%. In double infection cases, amniotic fluid IL-8 was very high （mean: 129.5ng/ml), and frequency of chronic lung disease was also very high （38.5%) compared to those in non-infected cases （5.6%). The duration from the amniocentasis to delivery in double infection cases was significantly shorter than that in no infection cases.
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[Journal Article] Multiple pregnancy,short cervix, part-time worker, steroid use, low educational level and male fetus are risk factors for preterm birth in Japan: A multicenter, prospective study.2013
Author(s)
Shiozaki, A., Yoneda S., Nakabayashi M., Takeda Y., Takeda S., Sugimura M., Yoshida K., Tajima A., Manabe M., Akagi K., Nakagawa S., Tada K., Imafuku N., Ogawa M., Mizunoe T., Kanayama N., Itoh H., Minoura S., Ogino M., Saito S.
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Journal Title
The Journal of Obstetrics and Gynaecology Research
Pages: 1-9
Peer Reviewed
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[Journal Article] A role for IL-17 in induction of an inflammation at fetomaternal interface in preterm labour.2010
Author(s)
Ito M., Nakashima A., Hidaka T., Okabe M., Bac N.D., Ina S., Yoneda S.,ShiozakiA., Sumi S., Tsuneyama K., Nikaido T., Saito S.
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Journal Title
J.Reprod Immunol.
Volume: 84
Pages: 75-85
Peer Reviewed
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