Analysis of pathophysiology of presbycusis by gene profile study of senescent cochlear cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659305
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KITAMURA Ken 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (90010470)
• Co-Investigator(Kenkyū-buntansha): NOGUCHI Yoshihiro 東京医科歯科大学, 医学部附属病院, 講師 (50282752) ; KIMURA Yurika 東京都健康長寿医療センター, 研究所, 研究院 (40450564) ; SAWABE Motoji 東京医科歯科大学, 医学部保健衛生学科, 教授 (30196331)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子 / 医療・福祉 / 神経科学 / 脳・神経

Research Abstract

Immunohistochemical study of cochlin demonstrated increased rate of positive area in the spiral ligament of the basal turn of the cochlea of the subjects at the age of older than 90 years than that of the age from 65 to 75 years. We succeeded to extract mRNA of COCH and SLC26A5 from archival formalin fixed paraffin embedded human cochlear cells by laser microdissection.

Research Products

• [Journal Article] Longitudinal study of 29 patients with Meniere's disease with follow-up of 10 years or more (2012)
  • Author(s): Sumi T, Watanabe I, Tsunoda A, Nishio A, Komatsuzaki A, Kitamura K
  • Journal Title: Acta Otolaryngol. Volume: 132 Pages: 10-5
  • DOI: doi:10.3109/00016489.2011.627570
  • Peer Reviewed
• [Journal Article] Anatomical feature of the middle cranial fossa in fetal periods: possible etiology of superior canal dehiscence syndrome. (2012)
  • Author(s): Takahashi N, Tsunoda A, Shirakura S, Kitamura K
  • Journal Title: Acta Otolaryngol Volume: 132 Pages: 385-90
  • DOI: doi:10.3109/00016489.2011.637234
  • Peer Reviewed
• [Journal Article] Extended screening for major mitochondrial DNA point mutations in patients with hereditary hearing loss. (2012)
  • Author(s): Kato T, Nishigaki Y, Noguchi Y, Fuku N, Ito T, Mikami E, Kitamura K and Tanaka M
  • Journal Title: J Hum Genet. Volume: 57 Pages: 772-5
  • DOI: doi:10.1038/jhg.2012.109
  • Peer Reviewed
• [Journal Article] Mitochondrial DNA haplogroup associated with hereditary hearing loss in a Japanese population. (2012)
  • Author(s): Kato T, Fuku N, Noguchi Y, Murakami H, Miyachi M, Kimura Y, Tanaka M and Kitamura K
  • Journal Title: Acta Otolaryngol. Volume: 132 Pages: 1178-82
  • DOI: doi:10.3109/00016489.2012.693624
  • Peer Reviewed
• [Journal Article] メトトレキサート関連リンパ増殖性疾患の4例. (2011)
  • Author(s): 西尾綾子、角卓郎、山口恵、桑波田悠子、喜多村健
  • Journal Title: 耳鼻臨床 Volume: 104 Pages: 143-50
  • DOI: doi:10.5631/jibirin.104.143
  • Peer Reviewed
• [Journal Article] Audiovestibular findings in a branchio-oto syndrome patient with a SIX1 mutation. (2011)
  • Author(s): Noguchi Y, Ito T, Nishio A, Honda K, Kitamura K
  • Journal Title: Acta Otolaryngol Apr Volume: 131(4) Pages: 413-8
  • DOI: doi:10.3109/00016489.2010.543146
  • Peer Reviewed
• [Journal Article] 高齢者における突発性難聴の聴力予後の検討 (2011)
  • Author(s): 山本容子,木村百合香,加藤智史,杉浦むつみ,喜多村健
  • Journal Title: OtologyJapan Volume: 21(2) Pages: 143-8
  • URL: http://mol.medicalonline.jp/library/archive/select?jo=ed7otolj
  • Peer Reviewed
• [Journal Article] 埋め込み型骨導補聴器の聴覚成績と術中、術後合併症の検討 (2011)
  • Author(s): 野口佳裕,高橋正時,喜多村健
  • Journal Title: 日耳鼻会報 Volume: 114 Pages: 607-14
  • DOI: doi:10.3950/jibiinkoka.114.607
  • Peer Reviewed
• [Journal Article] 先天性外耳道閉鎖症例における埋め込み型骨導補聴器(Bone-Anchored Hearing Aid: BAHA)の有効性に関する検討. (2011)
  • Author(s): 福島邦博,假谷伸,長安吏江,福田諭,小林俊光,喜多村健,熊川孝三,宇佐美真一,岩崎聡,土井勝美,暁清文,東野哲也,西崎和則
  • Journal Title: 日耳鼻会報 Volume: 114 Pages: 761-7
  • DOI: doi:10.3950/jibiinkoka.114.761
  • Peer Reviewed
• [Journal Article] Long term follow-up study of mastoid obliteration using bone pate in cholesteatoma. (2011)
  • Author(s): Kitamura K, Nakamura Y, Noguchi Y, Takahashi M
  • Journal Title: The Journal of International Advanced Otology Volume: 7(3) Pages: 42-3
  • Peer Reviewed
• [Journal Article] アレルギー性鼻炎患者における気管支喘息合併率調査と抗ロイコトリエン薬プランルカストの有効性に関する検討. (2011)
  • Author(s): 岩崎朱見,鈴木康弘,神山亮介,萩野幸治,戸叶尚史,喜多村健
  • Journal Title: Progress in Medicine Volume: 9(31) Pages: 2175-8
  • URL: http://mol.medicalonline.jp/library/journal/abstract?GoodsID=ai5prmda/2011/003109/027&name=2175-2178j&UserID=202.242.187.155
• [Journal Article] What do patients with hereditary deafness think of genetic studies? (2010)
  • Author(s): Abe S, Noguchi Y, Kitamura K
  • Journal Title: Auris Nasus Larynx Volume: 37 Pages: 422-426
  • URL: http://www.aurisnasuslarynx.com/
  • Peer Reviewed
• [Journal Article] Novel ATP6V1B1 mutations in distal renal tubular acidosis and hearing loss. (2010)
  • Author(s): Yashima T, Noguchi Y, Kawashima Y, Rai T, Ito T, Kitamura K
  • Journal Title: Acta Otolaryngol Volume: 130 Pages: 1002-1008
  • URL: http://informahealthcare.com/doi/abs/10.3109/00016481003631529
  • Peer Reviewed
• [Journal Article] Modified paraffin-embedding method for the human cochlea that reveals a fine morphology and excellent immunostaining results. (2010)
  • Author(s): Takahashi M, Kimura Y, Sawabe M, Kitamura K
  • Journal Title: Acta Otolaryngol Volume: 130 Pages: 788-792
  • URL: http://informahealthcare.com/doi/abs/10.3109/00016480903426626
  • Peer Reviewed
• [Journal Article] Quantitative cellular level analysis of mitochondrial DNA 3243A>G mutations in individual tissues from the archival temporal bones of a MELAS patient (2010)
  • Author(s): Koda H, Kimura Y, Ishige I, Eishi Y, Takahashi K, Iino Y, Kitamura K
  • Journal Title: Acta Otolaryngol Volume: 130 Pages: 344-350
  • URL: http://informahealthcare.com/doi/full/10.3109/00016480903148282
  • Peer Reviewed
• [Journal Article] Extensive and rapid screening for major mitochondrial DNA point mutations in patients with hereditary hearing loss. (2010)
  • Author(s): Kato T, Nishigaki Y, Noguchi Y, Ueno H, Hosoya H, Ito T, Kimura Y, Kitamura K and Tanaka M
  • Journal Title: J Hum Genet Volume: 55 Pages: 147-154
  • Peer Reviewed
• [Journal Article] RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection. -A technical report.
  • Author(s): Kimura Y, Kubo S, Koda H, Shigemoto K, Sawabe M, Kitamura K.
  • Journal Title: Hear Res(accepted)
  • URL: http://www.sciencedirect.com/science/journal/03785955
  • Peer Reviewed
• [Presentation] Gene Expression Analysis of Inner Ear Cells from Formalin Fixed Paraffin Embedded Archival Temporal Bone Section using Laser Microdissection. (2012)
  • Author(s): Kimura Y, Kubo S, Shigemoto K, Koda H, Sawabe M, Kitamura K
  • Organizer: The First Asian Otology Meeting & The 3rd East Asian Symposium on Otology.
  • Place of Presentation: Nagasaki
  • Year and Date: 20120600
• [Presentation] Mechanotransduction in Inner Ear Hair Cells Requires Transmembrane Channel-Like Genes 1 or 2. (2012)
  • Author(s): Kawashima Y, Geleoc G SG, Kurima K, Labay V, Lelli A, Asai Y, Makishima T, Wu D-K, Della Santina CC, Kitamura K, Holt JR, Griffith AJ
  • Organizer: The First Asian Otology Meeting & The 3rdEast Asian Symposium on Otology.
  • Place of Presentation: Nagasaki
  • Year and Date: 20120600
• [Presentation] Mitochondrial DNA Haplogroup Analysis Associated with Presbycusis in a Japanese Population. (2012)
  • Author(s): Kato T, Fuku N, Kimura Y, Tanaka M, Kitamura K
  • Organizer: The First Asian Otology Meeting & The 3rd East Asian Symposium on Otology.
  • Place of Presentation: Nagasaki
  • Year and Date: 20120600
• [Presentation] ADFNA5 mutation in two Japanese familieswith autosomal dominant hereditary hearing loss. (2011)
  • Author(s): Nishio A, Noguchi Y, Kitamura K
  • Organizer: 11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery.
  • Place of Presentation: Kobe
  • Year and Date: 20111200
• [Presentation] A case of left atypical Ramsay Hunt syndrome developing to left Wallenberg syndrome. (2011)
  • Author(s): Inaba Y, Suzuki Y, Tsunoda A, Kitamura K
  • Organizer: The 11th Japan-Taiw an Conference on Otolaryngology-Head and Neck Surgery.
  • Place of Presentation: Kobe
  • Year and Date: 20111200
• [Presentation] 遺伝性難聴疑い症例のミトコンドリアハプログループ解析. (2011)
  • Author(s): 加藤智史,野口佳裕,木村百合香,喜多村健
  • Organizer: 第21回日本耳科学会総会・学術講演会
  • Place of Presentation: 沖縄
  • Year and Date: 20111124-26
• [Presentation] Evaluation of Baha efficacy by self-assessment questionnaires. (2011)
  • Author(s): Noguchi Y, Sawada M, Takahashi M, Tokano H, Kitamura K
  • Organizer: The 8th Asia Pacific Symposium on Cochlear Implant and Related Sciences
  • Place of Presentation: Daegu Korea
  • Year and Date: 20111025-28
• [Presentation] Long term follow-up study of mastoid obliteration using bone pate in cholesteatoma. (2011)
  • Author(s): Kitamura K, Y Nakamura Y, Noguchi Y, Takahashi M
  • Organizer: 28th Politzer Society Meeting
  • Place of Presentation: Athens Greece
  • Year and Date: 20110928-1001
• [Presentation] Pathology of the superior canal dehiscence (SCD) from a point of human evolution. (2011)
  • Author(s): Takahashi N, Tsunoda A, Kitamura K
  • Organizer: Collegium Oto ? Rhino-Laryngologicum Amicitiae Sacrum
  • Place of Presentation: Bruges Belgium
  • Year and Date: 20110905-07
• [Presentation] does human evolution cause vertigo? (2011)
  • Author(s): Tsunoda A, Kitamura K, Takahashi N, Akita K, Yamaguchi K. The certain etiology of the superior canal dehiscence syndrome
  • Organizer: Joint Meeting of European association of Clinical Anatomy and British Association of Clinical Anatomists.
  • Place of Presentation: Poster, Padova Itary
  • Year and Date: 20110600
• [Presentation] めまい・難聴診断のUp-to-Date. (2011)
  • Author(s): 喜多村健
  • Organizer: 第109回日本耳鼻咽喉科学会福島県地方部会学術講演会
  • Place of Presentation: 福島
  • Year and Date: 2011-04-10
• [Presentation] Extensive and rapid screening for major mitochondrial DNA point mutations in patients with hereditary hearing loss. (2010)
  • Author(s): Kato T, Nishigaki Y, Fuku N, Ueno H, Noguchi Y, Kitamura K, Tanaka M
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Reseach and Medicine.
  • Place of Presentation: Fukuoka
  • Year and Date: 20101216-18
• [Presentation] Extensive and rapid screening for major mitochondrial DNA point mutations in patients with hereditary hearing loss. (2010)
  • Author(s): Kato T, Nishigaki Y, Noguchi Y, Ito T, Tanaka M, Kitamura K
  • Organizer: 2nd East Asian Symposium on Otology
  • Place of Presentation: Taipei Taiwan
  • Year and Date: 20101126-28
• [Presentation] Variable audiovestibular findings in patients with enlargement of the vestibular aqueduct caused bymutations of SLC26A4, SIX1, and ATP6V1B1. (2010)
  • Author(s): Noguchi Y, Ito T, Nishio A, Honda K, Kitamura K
  • Organizer: Sixth international symposium on Meniere's disease and inner ear disorders.
  • Place of Presentation: Kyoto
  • Year and Date: 20101114-17
• [Presentation] Modified paraffin-embedding method for human cochlea that reveals a fine morphology and excellent immuostaining results. (2010)
  • Author(s): Takahashi M, Kimura Y, Sawabe M, Kitamura K
  • Organizer: Twelfth Triennial Meeting The International Otopathology Society.
  • Place of Presentation: Boston USA
  • Year and Date: 20100606-08
• [Presentation] Audiovestibular findings in a Branchio-Oto syndrome patient with SIX1 mutation. (2010)
  • Author(s): Noguchi Y, Ito T, Nishio A, Kitamura K
  • Organizer: Collegium Oto-Rhino-Laryngologicum Amicitiae Sacrum
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2010-08-25
• [Book] 言語聴覚士のための聴覚障害学第1版 (2012)
  • Author(s): 喜多村健
  • Publisher: 医歯薬出版
• [Book] 第7章:聴覚・平衡機能系疾患の医療ニーズ (2012)
  • Author(s): 喜多村健
  • Total Pages: 1027-30
  • Publisher: 技術情報協会
• [Book] Epidemiology of Otitis Media with Effusion (OME) in Japan. (2012)
  • Author(s): Kitamura K
  • Total Pages: 151-8
  • Publisher: Koonja publishing Inc.
• [Remarks]
  • URL: http://www.tmd.ac.jp/oto/
• [Patent(Industrial Property Rights)] 自然骨と酷似した切削性を有する人工骨モデルの製造方法 (2013)
  • Inventor(s): 角田篤信、伊藤卓、喜多村健、大野秀則、杉山久幸
  • Industrial Property Rights Holder: 角田篤信、伊藤卓、喜多村健、大野秀則、杉山久幸
  • Industrial Property Number: 特許、5215350
  • Acquisition Date: 2013-03-08