In vitro study on comparison of epigenetic alteration patterns between periodontitis and a particular type of cancer models

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659381
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: KUBONIWA Masae 大阪大学, 歯学部附属病院, 講師 (00303983)
• Co-Investigator(Kenkyū-buntansha): OJIMA Miki 大阪大学, 大学院・歯学研究科, 助教 (20263303)
• Co-Investigator(Renkei-kenkyūsha): SEKINE Shinichi 大阪大学, 大学院・歯学研究科, 助教 (70506344)
• Project Period (FY): 2010 – 2011
• Keywords: 歯肉上皮細胞 / Porphyromonas gingivalis / 癌抑制遺伝子 / p53 / MDM2 / 糖代謝

Research Abstract

To assess influences of Porphyromonas gingivalis infection on p53 and MDM2(p53 E3 ubiquitin protein ligase) expression, western blot analyses were performed. The results indicated that P. gingivalis mild infection activated MDM2, and that resulted in p53 reduction. Furthermore, impaired p53 accelerated the glycolysis pathway in infected gingival epithelial cells in the same manner as in cancer cells.

Research Products

• [Journal Article] Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease (2012)
  • Author(s): Yoneda M, Naka S, Nakano K, Wada K, Endo H, Mawatari H, Imajo K, Nomura R, Hokamura K, Ono M, Murata S, Tohnai I, Sumida Y, Shima T, Kuboniwa M, Umemura K, Kamisaki Y, Amano A, Okanoue T, Ooshima T, Nakajima A
  • Journal Title: BMC Gastroenterol Volume: 12 Pages: 16
  • Peer Reviewed
• [Journal Article] Genotyping to distinguish microbial pathogenicity in periodontitis (2010)
  • Author(s): Kuboniwa M, Inaba H, Amano A
  • Journal Title: Periodontol 2000 Volume: 54 Pages: 136-59
  • Peer Reviewed
• [Journal Article] Subgingival biofilm formation (2010)
  • Author(s): Kuboniwa M, Lamont RJ
  • Journal Title: Periodontology 2000 Volume: 52 Pages: 38-52
  • Peer Reviewed
• [Journal Article] Effect of eucalyptus-extract chewing gum on oral malodor : a double-masked, randomized trial (2010)
  • Author(s): Tanaka M, Toe M, Nagata H, Ojima M, Kuboniwa M, Shimizu K, Osawa K, Shizukuishi S
  • Journal Title: Journal of Periodontology Volume: 81 Pages: 1564-1571
  • Peer Reviewed
• [Presentation] 口腔感染症研究への"マルチオミクス"の応用～現状と可能性～、口の難病プロジェクトアニュアルシンポジウム (2012)
  • Author(s): 久保庭雅恵
  • Organizer: 大阪大学歯学研究科弓倉記念ホール
  • Place of Presentation: 大阪
  • Year and Date: 2012-03-29
• [Presentation] エリスリトールがPorphyromonas gingivalisのメタボロームに及ぼす影響 (2012)
  • Author(s): 久保庭雅恵、橋野恵衣、Alghamdi Samar、長洋、天野敦雄
  • Organizer: 日本農芸化学会大会2012
  • Place of Presentation: 京都女子大学,京都
  • Year and Date: 2012-03-23
• [Presentation] マルチオミクスによる口腔感染症の分子基盤解析 (2012)
  • Author(s): 久保庭雅恵、天野敦雄
  • Organizer: 学術フロンティア推進事業シンポジウム歯周病による動脈硬化,糖尿病,低体重児出産の誘発機序の解明とその制御
  • Place of Presentation: 日本大学松戸歯学部、千葉
  • Year and Date: 2012-02-18
• [Presentation] 予測医歯学への"マルチオミクス"の応用～現状と可能性～ (2011)
  • Author(s): 久保庭雅恵
  • Organizer: 大阪府病院診療所歯科部長会第3回研修会
  • Place of Presentation: ホテルモントレ大阪、大阪
  • Year and Date: 2011-12-03
• [Presentation] メタボロミクス解析によるエリスリトール存在下でのPorphyromonas gingivalis代謝プロファイルの検討 (2011)
  • Author(s): 橋野恵衣、久保庭雅恵、黒田裕美子、前田和彦、小島美樹、永田英樹、天野敦雄
  • Organizer: 第60回日本口腔衛生学会・総会
  • Place of Presentation: 日本大学松戸歯学部、千葉
  • Year and Date: 2011-10-10
• [Presentation] Porphyromonas gingivalis GAPDHはStreptococcus oralis GAPDHに特異的に結合する (2011)
  • Author(s): 前田和彦、久保庭雅恵、小島美樹、黒田裕美子、橋野恵衣、東江正裕、永田英樹
  • Organizer: 第60回日本口腔衛生学会・総会
  • Place of Presentation: 日本大学松戸歯学部,千葉
  • Year and Date: 2011-10-09
• [Presentation] Metabolome profiles in Porphyromonas gingivalis under erythritol stress (2011)
  • Author(s): Hashino E, Kuboniwa M, Amano A
  • Organizer: The 59th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Hiroshima
  • Year and Date: 2011-10-08
• [Presentation] パラアミノ安息香酸によるPorphyromonas gingivalisの菌体内メタボロームプロファイルの変化 (2011)
  • Author(s): 久保庭雅恵、Alghamdi Samar、橋野恵衣、天野敦雄
  • Organizer: 第53回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 2011-10-02
• [Presentation] Porphyromonas gingivalisのリンゴ酸デヒドロゲナーゼはバイオフィルム形成に関与する (2011)
  • Author(s): 前田和彦、永田英樹、小島美樹、久保庭雅恵、天野敦雄
  • Organizer: 第22回近畿・中国・四国口腔衛生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2011-10-02
• [Presentation] Multiple signalling pathways are involved in apoptosis and cell cycle arrest induced by periodontal pathogen in human trophoblast (2011)
  • Author(s): Inaba H, Kuboniwa M, Sugita H, Amano A.
  • Organizer: EMBO meeting
  • Place of Presentation: Vienna(Austria)
  • Year and Date: 2011-09-11
• [Presentation] Metabolome profiles in Streptococcus gordonii co-cultured with Porphyromonas gingivalis (2011)
  • Author(s): Kuboniwa M, Kuroda Y, Hashino E, Alghamdi S, Hackett M, Lamont RJ, Amano A.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-10
• [Presentation] Porphyromonas gingivalis NAD-dependent glutamate dehydrogenase binds to glyceraldehyde-3-phosphate dehydrogenase of Streptococcus oralis (2011)
  • Author(s): Maeda K, Nagata H, Kuboniwa M, Ojima M,
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-10
• [Presentation] Remnant matrix of Porphyromonas gingivalis biofilms treated with chlorhexidine (2011)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Maezono H, Yamamoto R, Asahi Y, Kuremoto K, Ebisu S.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-09
• [Presentation] Metabolome profiles in Porphyromonas gingivalis stimulated with exogenous para-aminobenzoate (2011)
  • Author(s): Kuboniwa M, Alghamdi S, Hashino E, Lamont RJ, Amano A.
  • Organizer: 7^<th> International Conference of the Metabolomics Society
  • Place of Presentation: Caines, Australia
  • Year and Date: 2011-07-29
• [Presentation] 細菌感染症の転帰を決める細胞ロジスティクス機構の分子基盤解析 (2011)
  • Author(s): 天野敦雄, 久保庭雅恵, 古田信道
  • Organizer: 新学術領域「細胞内ロジスティクス」第3回班会議
  • Place of Presentation: 鳥羽シーサイドホテル、三重
  • Year and Date: 2011-06-01
• [Presentation] Role of 4-aminobenzoate in interspecies communication (2010)
  • Author(s): Kuboniwa M, Chawla A, Hashino E, Nagata H, Amano A, Hackett M, Lamont RJ, Shizukuishi S
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Effect of erythritol on periodontopathic biofilm development (2010)
  • Author(s): Hashino E, Kuboniwa M, Iwasaki M, Yamamoto Y, Suematsu K, Cho H, Shizukuishi S
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Exopolysaccharide accumulation and antimicrobial effect in Porphyromonas gingivalis biofilm (2010)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Maezono H, Yamamoto R, Asahi Y, Ebisu S
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] gordonii in Periodontal Disease (2010)
  • Author(s): Lamont RJ, Verma R, Bainbridge B, Demuth D, Kuboniwa M, Yehia B, Eastman C, Rivera M, Bhattacharyya I, Kesavalu L., In vivo Interaction of P. gingivalis with S
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-14
• [Presentation] Proteomics of a Model Oral Microbial Community (2010)
  • Author(s): Hackett M, Lamont RJ, Kuboniwa M, Hendrickson E, Xia Q, Wang T, Beck DAC
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-14
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/-prevent/research04.html