Activities of cerebellar neurons in behaving mice

2012 Fiscal Year Final Research Report

• Project/Area Number: 22680031
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMURA Kazuo 東京大学, 大学院・医学系研究科, 准教授 (60423159)
• Project Period (FY): 2010 – 2012
• Keywords: 神経科学 / 生理学 / パッチクランプ / 光子イメージング

Research Abstract

To elucidate the mechanism of cerebellar dependent motor control and motor learning, we have developed an efficient motor task apparatus for mice to perform licking and mechanical lever-pushing under head-fixed configuration. Furthermore, we have shown that synchrony of climbing fiber responses in Purkinje cells correlate with the longitudinal stripes of cerebellar cortex at single cell level.

Research Products

• [Journal Article] Spatiotemporal Dynamics of Functional Clusters of Neurons in the Mouse Motor Cortex during a Voluntary Movement. (2013)
  • Author(s): Hira, R., Ohkubo, F., Ozawa, K., Isomura, Y., Kitamura, K., Kano, M., Kasai, H. & Matsuzaki, M.
  • Journal Title: J. Neurosci Volume: 33 Pages: 1377-1390
  • Peer Reviewed
• [Journal Article] Dendritic calciumsignaling in cerebellar Purkinje cell (2012)
  • Author(s): Kitamura, K. & Kano, M.
  • Journal Title: Neural Networks published online
  • Peer Reviewed
• [Journal Article] GABAergic inhibition regulates developmental synapse elimination in the cerebellum. (2012)
  • Author(s): Nakayama, H., Miyazaki, T., Kitamura, K., Hashimoto, K., Yanagawa, Y., Obata, K., Sakimura, K., Watanabe, M. & Kano, M.
  • Journal Title: Neuron Volume: 74 Pages: 384-396
  • Peer Reviewed
• [Journal Article] Matsuki, N. & Ikegaya, Y. Locally synchronized synaptic inputs. (2012)
  • Author(s): Takahashi, N., Kitamura, K., Matsuo, N., Mayford, M., Kano, M.
  • Journal Title: Science Volume: 335 Pages: 353-356
  • Peer Reviewed
• [Journal Article] 小脳 プルキンエ細胞樹状突起活動の in vivo イメージング (2012)
  • Author(s): 喜多村和郎,橋本浩一,狩野方伸
  • Journal Title: 生体の科学 Volume: 63 Pages: 26-33
• [Journal Article] Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo. (2011)
  • Author(s): Kitamura, K. & Hausser, M.
  • Journal Title: J. Neurosci.31 Pages: 10847-10858
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca2+ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum. (2011)
  • Author(s): Hashimoto, K., Tsujita, M.Miyazaki, T., Kitamura, K., Yamazaki, M., Shin, H.-S., Watanabe, M., Sakimura, K. & Kano, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 2447-2457
  • Peer Reviewed
• [Journal Article] Invivo two-photonuncagingofglutamaterevealingthestructure-functionrelationshipsofdendriticspinesintheneocortexofadultmice. (2011)
  • Author(s): Noguchi,J.,Nagaoka,A.,Watanabe,S.,Ellis-Davies,G.C.,Kitamura,K.,Kano,M.,Matsuzaki,M.&Kasai,H.
  • Journal Title: J.Physiol Volume: 589 Pages: 2447-2457
  • Peer Reviewed
• [Journal Article] Calcium-dependentregulationofclimbingfibersynapseeliminationduringpostnatalcerebellardevelopment.
  • Author(s): Kano,M.,Nakayama,H.,Hashimoto,K.,Kitamura,K.,Sakimura,K.&Watanabe,M.
  • Journal Title: J. Physiol.inpress
  • Peer Reviewed
• [Presentation] マウスバレル皮質における感覚シナプス入力の可視化 (2013)
  • Author(s): 喜多村和郎、狩野方伸
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130327-0329
• [Presentation] マウス小脳における登上線維応答の同期性とAldolaseC 発現パ ターンとの関連 (2013)
  • Author(s): 堤新一郎、多田真弓、崎村建司、喜多村 和郎、狩野方伸
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130327-0329
• [Presentation] Climbing fiber network refinement and desynchronization of Purkinje cell population activity during postnatal cerebellar development. (2013)
  • Author(s): Jean-Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano.
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130327-0329
• [Presentation] Visualization of an active ensemble of cortical neurons in vivo using a novel activity-dependent promoter E-SARE. (2012)
  • Author(s): T. KAWASHIMA, K. KITAMURA, K. OHKI, K. SUZUKI, M. NONAKA, S. KAMIJO, S. TAKEMOTO-KIMURA, M. KANO, H. OKUNO, H. BITO.
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans, USA.
  • Year and Date: 20121013-1017
• [Presentation] Postnatal climbing fiber synapse remodeling desynchronizes population activity of Purkinje cells in developing cerebellum. (2012)
  • Author(s): Jean- Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-0921
• [Presentation] 新規活動依存的プロモーターE- SARE を用いた、大脳皮質における機 能的回路の同定. (2012)
  • Author(s): 川島 尚之、喜多村 和郎、大木 研一、 鈴木 敢三、 野中 美応、上條 諭志、竹 本 さやか、狩野 方伸、 奥野 浩行、尾 藤 晴彦.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-0921
• [Presentation] Heterogeneous organization of individual synaptic inputs in the mouse barrel cortex. (2012)
  • Author(s): Kazuo Kitamura, Yuji Ikegaya, Masanobu Kano.
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 20120714-0718
• [Presentation] Spatial And Temporal Structure Of Cortical Microcircuit Activity For Generating Voluntary Movement. (2012)
  • Author(s): Hira R., Ohkubo F., Ozawa K., Isomura Y., Kitamura K., Kano M., Kasai H. & Matsuzaki M.
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 20120714-0718
• [Presentation] 光子イメージング による単一ニューロン・単一シナプスの 活動計測. (2012)
  • Author(s): 喜多村和郎.生体内 2
  • Organizer: 第2回睡眠研究会
  • Place of Presentation: 名古屋
  • Year and Date: 20120705-0706
• [Presentation] 大脳皮質における感覚シナプス入力の可視化. (2012)
  • Author(s): 喜多村和郎.
  • Organizer: 生理研研究会「シナプス可塑性の動作原理?分子から行動で?」
  • Place of Presentation: 岡崎
  • Year and Date: 20120614-0615
• [Presentation] Two-photon imaging of neuronal population activities in cerebellar cortex. (2011)
  • Author(s): Kazuo Kitamura.
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-1216
• [Presentation] Heterogeneous organization of sensory synaptic inputs in the mouse barrel cortex. (2011)
  • Author(s): Kazuo Kitamura.
  • Organizer: 生理研国際研究集会
  • Place of Presentation: 岡崎
  • Year and Date: 20111208-1209
• [Presentation] Enhanced synchrony of climbing fiber-induced Ca2+ signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2 (GluRδ2) knockout mouse. (2011)
  • Author(s): Miki Hashizume, Kazuo, Kitamura, Kenji Sakimura and Masanobu Kano.
  • Organizer: Neuro2011
  • Place of Presentation: 横浜
  • Year and Date: 20110914-0917
• [Presentation] Two-photonimagingofPurkinjecelldendriticactivityinvivo. (2011)
  • Author(s): KazuoKitamura.
  • Organizer: 8thIBROWorldCongress
  • Place of Presentation: Florence,Itary
  • Year and Date: 20110714-0718
• [Presentation] DendriticactivitiesofcerebellarPurkinjecellinvivo (2011)
  • Author(s): K.Kitamura&M.Hausser.
  • Organizer: 第88回日本生理学大会
  • Year and Date: 2011-03-28
• [Presentation] 樹状突起活動の in vivo イメージングと光操作. (2010)
  • Author(s): 喜多村和郎.
  • Organizer: 第2回光操作研究会
  • Place of Presentation: 岡崎
  • Year and Date: 20100909-0910
• [Presentation] 登上線維の発達過程における P/Q 型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一、辻田実加、喜多村和郎、宮崎 太輔、山崎真弥、Hee-Sup Shin、渡辺雅 彦、崎村建司、狩野方伸.
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 20100902-0904
• [Presentation] GluD2(GluRδ2)ノックアウトマウスの小脳プルキンエ細胞集団の2 光子カルシウムイメージング解析. (2010)
  • Author(s): 橋爪幹、喜多村和郎、崎村建司、狩野方伸
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 20100902-0904
• [Presentation] Simultaneous two-photon calcium imaging and patch-clamp recordings in vivo (2010)
  • Author(s): K. Kitamura, M. Hausser & M. Kano
  • Organizer: Jacques Monod Conference"Imaging brain circuits in health and disease
  • Place of Presentation: Roscoff, France
  • Year and Date: 20100630-0704
• [Presentation] 生体内における神経活動 の2光子イメージング (2010)
  • Author(s): 喜多村和郎
  • Organizer: 第5回日本分子 イメージング学会総会・学術集会
  • Place of Presentation: 大津
  • Year and Date: 20100522-0523
• [Presentation] 小脳登上線維?プルキンエ細胞シナプスの生後発達に おける P/Q 型電位依存性カルシウムチャ ネルの役割. (2010)
  • Author(s): 橋本浩一、辻田実加、宮崎太輔、山崎真 弥、喜多村和郎、Hee-Sup Shin、渡辺雅 彦、崎村建司、狩野方伸.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 20100519-0521
• [Book] 脳神経科学イラストレイテッド (2013)
  • Author(s): 喜多村和郎.
  • Publisher: invivoイメージング羊土社
• [Book] 最新パッチクランプ実験 技術法,岡田泰伸 編,吉岡書店 (2013)
  • Author(s): 喜多村和郎.
  • Publisher: in vivo イメージングパッチ法
• [Book] Patch Clamp Techniques (Y. Okada ed.), Springer (2012)
  • Author(s): Kazuo Kitamura.
  • Publisher: Two-photon targeted patch-clamp recordings in vivo