2013 Fiscal Year Final Research Report
Glycoproteomic identification of carbohydrate targeting lung cancer biomarkers
| Project/Area Number |
22680064
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
UEDA Koji 独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (10509110)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 腫瘍マーカー / プロテオミクス / 糖鎖 / 肺癌 |
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| Research Abstract |
To develop novel carbohydrate-targeting biomarkers for early detection of lung cancer, glycoproteomic profiling of 144 serum samples was conducted. We further established a mass spectrometric quantification method for glycan structure alterations, named Energy resolved oxonium ion monitoring (Erexim) technology, in order for large-scaled validation study of 16 identified lung cancer biomarker candidates. The Erexim-based validation experiment using 87 independent serum samples revealed that frequency of alpha-1, 6 fucosylation on CD163-Asn105, Asn140, and Asn1027 was significantly up-regulated in even stage-I or II lung adenocarcinoma patients compared to normal control group. The Erexim technology was patented in US (US 8653448, 18.Feb.2014) and already utilized in various areas, such as chemistry, manufacturing and control (CMC) of biologics.
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Research Products
(49 results)
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[Journal Article] SMYD2-dependent HSP90 methylation promotes cancer cell proliferation by regulating the chaperonin complex formation2014
Author(s)
G. Toyokawa, M. Yoshimatsu, M. Nakakido, V. Saloura, K. Sone, L. Piao, H. S. Cho, K. Ueda, Y. Maehara, Y. Nakamura, and R. Hamamoto
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Journal Title
Cancer letters
Volume: (in press)
Peer Reviewed
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[Journal Article] Lysyl 5-Hydroxylation, a Novel Histone Modification, by Jumonji Domain Containing 6 (JMJD6)2013
Author(s)
M. Unoki, A. Masuda, N. Dohmae, K. Arita, M. Yoshimatsu, Y. Iwai, Y. Fukui, K. Ueda, R. Hamamoto, M. Shirakawa, H. Sasaki, and Y. Nakamura
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Journal Title
The Journal of biological chemistry
Volume: 288(9)
Pages: 6053-62
DOI
Peer Reviewed
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[Journal Article] Plasma Low-Molecular-Weight Proteome Profiling Identified Neuropeptide-Y as a Prostate Cancer Biomarker Polypeptide2013
Author(s)
K. Ueda, A. Tatsuguchi, N. Saichi, A. Toyama, K. Tamura, M. Furihata, R. Takata, S. Akamatsu, M. Igarashi, M. Nakayama, T. A. Sato, O. Ogawa, T. Fujioka, T. Shuin, Y. Nakamura, and H. Nakagawa
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Journal Title
Journal of proteome research
Volume: 12(10)
Pages: 4497-4506
DOI
Peer Reviewed
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[Journal Article] The oncogenic polycomb histone methyltransferase EZH2 methylates lysine 120 on histone H2B and competes ubiquitination2013
Author(s)
M. Kogure, M. Takawa, V. Saloura, K. Sone, L. Piao, K. Ueda, R. Ibrahim, T. Tsunoda, M. Sugiyama, Y. Atomi, Y. Nakamura, and R. Hamamoto
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Journal Title
Neoplasia
Volume: 15(11)
Pages: 1251-61
Peer Reviewed
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[Journal Article] Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression2012
Author(s)
M. Takawa, H. S. Cho, S. Hayami, G. Toyokawa, M. Kogure, Y. Yamane, Y. Iwai, K. Maejima, K. Ueda, A. Masuda, N. Dohmae, H. I. Field, T. Tsunoda, T. Kobayashi, T. Akasu, M. Sugiyama, S. Ohnuma, Y. Atomi, B. A. Ponder, Y. Nakamura, and R. Hamamoto
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Journal Title
Cancer research
Volume: 72(13)
Pages: 3217-27
DOI
Peer Reviewed
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[Journal Article] A comprehensive peptidome profiling technology for the identification of early detection biomarkers for lung adenocarcinoma2011
Author(s)
K. Ueda, N. Saichi, S. Takami, D. Kang, A. Toyama, Y. Daigo, N. Ishikawa, N. Kohno, K. Tamura, T. Shuin, M. Nakayama, T. A. Sato, Y. Nakamura, and H. Nakagawa
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Journal Title
PLoS One
Volume: 6(4)
Pages: e18567
DOI
Peer Reviewed
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[Journal Article] C12orf48, termed PARP-1 binding protein, enhances poly(ADP-ribose) polymerase-1 (PARP-1) activity and protects pancreatic cancer cells from DNA damage2011
Author(s)
L. Piao, H. Nakagawa, K. Ueda, S. Chung, K. Kashiwaya, H. Eguchi, H. Ohigashi, O. Ishikawa, Y. Daigo, K. Matsuda, and Y. Nakamura
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Journal Title
Genes Chromosomes Cancer
Volume: 50(1)
Pages: 13-24
DOI
Peer Reviewed
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[Journal Article] Development of serum glycoproteomic profiling technique; simultaneous identification of glycosylation sites and site-specific quantification of glycan structure changes2010
Author(s)
K. Ueda, S. Takami, N. Saichi, Y. Daigo, N. Ishikawa, N. Kohno, M. Katsumata, A. Yamane, M. Ota, T. A. Sato, Y. Nakamura, and H. Nakagawa
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Journal Title
Mol Cell Proteomics
Volume: 9(9)
Pages: 1819-28
DOI
Peer Reviewed
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[Journal Article] Involvement of the tubulin tyrosine ligase-like family member 4 polyglutamylase in PELP1 polyglutamylation and chromatin remodeling in pancreatic cancer cells2010
Author(s)
K. Kashiwaya, H. Nakagawa, M. Hosokawa, Y. Mochizuki, K. Ueda, L. Piao, S. Chung, R. Hamamoto, H. Eguchi, H. Ohigashi, O. Ishikawa, C. Janke, Y. Shinomura, and Y. Nakamura
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Journal Title
Cancer research
Volume: 70(10)
Pages: 4024-33
DOI
Peer Reviewed
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[Presentation] Serum biomarker identification by quantitative focused proteomics technologies2011
Author(s)
K. Ueda, M. Ishihara, H. Takahashi, O. Ishikawa, S. Hirono, H. Yamaue, N. Mizuno, K. Yamao, K. Hanada, T. Kimura, M. Gotoh, N. Araya, T. Sato, A. Utsunomiya, Y. Yamano, Y. Nakamura, and H. Nakagawa
Organizer
第70回日本癌学会学術総会
Place of Presentation
Nagoya, Japan
Year and Date
2011-10-04
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[Remarks] 日経産業新聞2013年9月20日 朝刊 10面
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[Remarks] 薬事日報2012年11月21日 朝刊 14面
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[Remarks] 薬事日報2013年9月26日 オンライン版
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