2013 Fiscal Year Final Research Report
Development of Novel Fluorogenic Probes for Labeling Intracellular Proteins
| Project/Area Number |
22685016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
HORI Yuichiro 大阪大学, 工学(系)研究科(研究院), 助教 (00444563)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | 蛋白質標識 / 生細胞蛍光イメージング / 発蛍光プローブ / PYPタグ |
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| Research Abstract |
Fluorescence protein labeling by a specific pair of a protein tag and synthetic probe is a powerful chemistry-based approach to investigate protein function and localization inside living cells. In this research, we developed a protein labeling system based on fluorogenic probes and photoactive yellow protein (PYP) tag. By utilizing this technique, we were succeeded in specific imaging of proteins inside living cells with high S/N ratio and excellent labeling kinetics. Furthermore, the labeling technique was applied to the imaging of DNA methylation.
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Research Products
(22 results)
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[Journal Article] Development of Fluorogenic Probes for Quick No-Wash Live-Cell Imaging of Intracellular Proteins2013
Author(s)
Hori, Y., Norinobu, T., Sato, M., Arita, K., Shirakawa, M., Kikuchi, K.
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Journal Title
J. Am. Chem. Soc.
Volume: 135
Pages: 12360-12365
DOI
Peer Reviewed
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