2011 Fiscal Year Final Research Report
Quantitative analysis of reciprocal onnection between the thalamus and neocortex by using lentiviral vectors
| Project/Area Number |
22700367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
HIOKI Hiroyuki 京都大学, 大学院・医学研究科, 助教 (00402850)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 神経回路網 |
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| Research Abstract |
By using" Double Lentiviral Vector Tet-Off Platform(Hioki et al., 2009),"we developed dual expression system with bidirectional TRE-tight promoter(TREB), internal ribosomal entry site(IRES) derived from encephalomyocarditis virus or 2Asequence of foot and mouth disease virus. We compared the expression level of transgenes in vivo, and revealed that 2A sequence was suitable for dual expression.
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[Journal Article] A Morphological Analysis of Thalamocortical Axon Fibers of Rat Posterior Thalamic Nuclei : A Single Neuron Tracing Study with Viral Vectors.
Author(s)
Ohno S, Kuramoto, E, Furuta, T, Hioki H, Tanaka Y, Fujiyama F, Sonomura T, Uemura M, Sugiyama K, Kaneko T
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Journal Title
Cereb Cortex
Volume: (in press)
DOI
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[Presentation] Subcolumnar structures in local inputs of pyramidalneurons onto corticothalamic neurons in rat barrel cortex2010
Author(s)
Tanaka Y, Tanaka Y, Konno M, Fujiyama F, Okamoto-Furuta K, Sonomura T, Kameda H, Hioki H, Furuta T, Nakamura KC, Kaneko T.
Organizer
40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-16
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[Presentation] LENTIVIRAL VECTORS DRIVE HIGH-LEVEL TRANSGENE EXPRESSION IN MARMOSET BRAINS2010
Author(s)
Watakabe A, Takaji M, Nakagami Y, Hioki H, Kaneko T, Kato S, Koboyashi K, Kawashima T, Okuno H, Bito H, Kitamura Y, Yamamori T
Organizer
40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-16
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