The molecular basis of interactions between cancer cells and neurons in glioma invasion

2012 Fiscal Year Final Research Report

• Project/Area Number: 22700375
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHUO Takuya 東京大学, 医科学研究所, 特任研究員 (90399006)
• Project Period (FY): 2010 – 2012
• Keywords: 脳腫瘍 / 神経膠腫細胞 / 神経細胞 / メタロプロテアーゼ / プロテオグリカン / 糖鎖 / 質量分析 / MALDI(マトリックス支援レーザー脱離イオン化法)

Research Abstract

To understand the molecular basis of interactions between glioma and neurons, we investigated the role of MT-MMP/neuroglycan C axis. In this study, we found that a recombinant MT-MMP catalytic domain could actually cleave full-length neuroglycan C in two fragments. In addition, we developed a new MALDI-mass spectrometric technique to analyze glycopeptides in a highly sensitive manner, and then we used the method to evaluate glycosylation status of MT-MMP.

Research Products

• [Journal Article] MZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis contains a novel noncanonical pleckstrin homology domain (2013)
  • Author(s): Nagano M, Hoshino D, Koshiba S, Shuo T, Koshikawa N, Tomizawa T, Hayashi F, Tochio N, Harada T, Akizawa T, Watanabe S, Handa N, Shirouzu M, Kigawa T, Yokoyama S, Seiki
  • Journal Title: J Biol Chem Volume: 286巻 Pages: 1598-31609
  • DOI: DOI:10.1074/jbc.M110.199430
  • Peer Reviewed
• [Journal Article] Detection of the heterogeneous O-glycosylation profile of MT1-MMP expressed in cancer cells by a simple MALDI-MS method
  • Author(s): Shuo T, Koshikawa N, Hoshino D, Minegishi T, Ao-Kondo H, Oyama M, Sekiya S, Iwamoto S, Tanaka K, Seiki M
  • Journal Title: PLoS One Volume: 7巻
  • DOI: DOI:10.1371/journal.pone.0043751
  • Peer Reviewed
• [Presentation] 液体マトリックス3AQ/CHCAを用いた新規MALDI質量分析法による膜型プロテアーゼMT1-MMPのO-結合型糖鎖修飾の解析 (2012)
  • Author(s): 周尾卓也
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県福岡市)
  • Year and Date: 2012-12-15
• [Presentation] Detection of the heterogeneous (2012)
  • Author(s): Takuya Shuo
  • Organizer: O-glycosylation of MT1-MMP expressed in cancer cells by a simple MALDI-MS method19th International Mass SpectrometryConference,2
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2012-09-16
• [Presentation] Detection of the heterogeneousO-glycosylation of MT1-MMP expressed in cancer cells by a simple MALDI-MS method (2012)
  • Author(s): 周尾卓也
  • Organizer: 第12回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学(東京都文京区)
  • Year and Date: 2012-06-30
• [Presentation] Glycan characterization of MT1-MMP (2011)
  • Author(s): Takuya Shuo
  • Organizer: an invasion-promoting metalloproteinase, by MALDI-digital ion trap mass spectrometrySeventh General Meeting of the International Proteolysis Society
  • Place of Presentation: Hilton San Diego Resort and Spa(San Diego/USA)
  • Year and Date: 2011-10-19
• [Presentation] MALDI質量分析における液体マ トリックス 3-AQ/CHCAを用いた細胞膜貫通型メタロプロテアーゼ MT1-MMP の O-結 合型糖鎖修飾の解析 (2011)
  • Author(s): 周尾卓也
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府吹田市)
  • Year and Date: 2011-09-14
• [Remarks] 東京大学[広報・情報公開]記者発表一覧
  • URL: http://www.u-tokyo.ac.jp/public/public01_240823_j.html
• [Remarks] 東京大学クロールCOE研究成果一覧
  • URL: http://www.u-tokyo.ac.jp.fiw.st/coe/japanese/achievements/category1/base3/report03-03.htm