Clarification of epileptogenicity using human brain slices in vitro

2011 Fiscal Year Final Research Report

• Project/Area Number: 22700376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Niigata University
• Principal Investigator: KITAURA Hiroki 新潟大学, 脳研究所, 助教 (80401769)
• Research Collaborator: KAKITA Akiyoshi 新潟大学, 脳研究所, 教授 (80281012) ; FUKUDA Masafumi 新潟大学, 医歯学総合病院, 講師 (00361907) ; KAMEYAMA Shigeki 国立病院機構, 西新潟中央病院, 院長
• Project Period (FY): 2010 – 2011
• Keywords: てんかん

Research Abstract

Epilepsy is one of the major neurological disorders in Japan. It is believed that hyper-excitability of the pyramidal neurons in the cerebral cortex contributes to epileptogenicity. However, the precise mechanism of the alteration was not still clarified. We struggled to observe spatiotemporal dynamics of epileptic activities using human brain slices taken from epileptic patients. As a result, we found unique epileptiform propagation of the activities in epileptogenic tissues but not in control tissues.

Research Products

• [Journal Article] Periventricular nodular heterotopia functionally couples with the overlying hippocampus. (2012)
  • Author(s): Kitaura H, Oishi M, Takei N, Fu YJ, Hiraishi T, Fukuda M, Takahashi H, Shibuki K, FujiiY, Kakita A
  • Journal Title: Epilepsia Volume: (in press)
  • DOI: DOI:10.1111/j.1528-1167.2012.03509.x
  • Peer Reviewed
• [Journal Article] Epidermoid cyst involving the medial temporal lobe : surgical pathologic features of the epileptogenic lesion. (2012)
  • Author(s): Hiraishi T, Oishi M, Kitaura H, Ryufuku M, Fu YJ, Fukuda M, Takahashi H, Fujii Y, Kakita A
  • Journal Title: Neturopathology Volume: 32(2) Pages: 196-201
  • DOI: DOI:10.1111/j.1440-1789.2011.01243x
  • Peer Reviewed
• [Journal Article] Spatiotemporal dynamics of epileptiform propagations : imaging of human brain slices. (2011)
  • Author(s): Kitaura H, Hiraishi T, Murakami H, Masuda H, Fukuda M, Oishi M, Ryufuku M, Fu Y, Takahashi H, Kameyama S, Fujii Y, Shibuki K, Kakita A
  • Journal Title: Neuroimage Volume: 58 Pages: 50-59
  • DOI: DOI:10.1016/j.neuroimage.2011.05.046
  • Peer Reviewed
• [Journal Article] Balloon cells in the dentate gyrus in hippocampal sclerosis associated with non-herpetic acute limbic encephalitis. (2011)
  • Author(s): Miyahara H, Ryufuku M, Fu YJ, Kitaura H, Murakami H, Masuda H, Kameyama S, Takahashi H, Kakita A
  • Journal Title: Seizure Volume: 20 Pages: 87-89
  • DOI: DOI:10.1016/j.seizure.2010.09.013
  • Peer Reviewed
• [Journal Article] Hypertrophy of hippocampal end folium neurons in patients with mesial temporal lobe epilepsy. (2011)
  • Author(s): Ryufuku M, Toyoshima Y, Kitaura H, Zheng Y, Fu YJ, Miyahara H, Murakami H, Masuda H, Kameyama S, Takahashi H, Kakita A
  • Journal Title: Neuropathology Volume: 31(5) Pages: 476-485
  • DOI: DOI:10.1111/j.1440-1789.2010.01191.x
  • Peer Reviewed
• [Journal Article] Transcranial imaging of somatotopic map plasticity after tail cut in mice. (2010)
  • Author(s): Kitaura H, Hishida R, Shibuki K
  • Journal Title: Brain Res Volume: 1319 Pages: 54-59
  • DOI: DOI:10.1016/j.brainres.2010.01.020
  • Peer Reviewed
• [Presentation] Pathophysiological mechanisms underlying hippocampus sclerosis for MTLE : implications of flavoprotein fluorescence imaging on human brain slices (2011)
  • Author(s): H. Kitaura
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington DC,(米国)
  • Year and Date: 2011-11-14
• [Presentation] 内側側頭葉てんかんにおける海馬硬化症の病態生理学的意義:ヒト脳スライス標本を用いたイメージング解析 (2011)
  • Author(s): 北浦弘樹
  • Organizer: 第45回日本てんかん学会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2011-10-06
• [Presentation] Spatiotemporal dynamics of epileptiform propagations in human partial epilepsy in vitro (2010)
  • Author(s): H. Kitaura
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego,(米国)
  • Year and Date: 2010-11-15
• [Presentation] 新皮質てんかん病巣における興奮伝播様式の解析:ヒト脳スライス標本を用いた光学的イメージング (2010)
  • Author(s): 北浦弘樹
  • Organizer: 第44回日本てんかん学会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2010-10-14
• [Presentation] 二次性新皮質てんかんの興奮動態:ヒト脳スライス標本を用いた解析 (2010)
  • Author(s): 北浦弘樹
  • Organizer: 第51回日本神経病理学会総会
  • Place of Presentation: シェーンバッハサボー(東京)
  • Year and Date: 2010-04-24