Establishment of producing method for detection antibody for primate using DNA immunization technology

2012 Fiscal Year Final Research Report

• Project/Area Number: 22700455
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: TOUGAN Takahiro 大阪大学, 微生物病研究所, 助教 (20379093)
• Co-Investigator(Renkei-kenkyūsha): HORII Toshihiro 大阪大学, 微生物病研究所, 教授 (80142305) ; YASUTOMI Yasuhiro 独立行政法人医薬基盤研究所, 霊長類医科学研究センター, センター長 (90281724) ; TACIBANA Taro 大阪市立大学, 工学(系)研究科(研究院), 准教授 (80311752) ; NKAMURA Shota 大阪大学, 微生物病研究所, 助教 (90432434)
• Project Period (FY): 2010 – 2012
• Keywords: リスザル / モデル動物 / 免疫関連遺伝子 / イムノグロブリン / Fcγレセプター / 検出抗体 / 次世代シークエンサー / マラリア

Research Abstract

The squirrel monkey (Saimiri sciureus) is a valuable experimental animal for research into infectious diseases such as malaria vaccine development; however, the IgG molecules in this animal are not yet to be characterized. Antibody-dependent cellular cytotoxity is an antibody-mediated immune response and is important for exclusion of pathogens or cancer cells. In human, IgG1 or IgG3 interacts with the Fc receptor, FcγRIIIa. Previous studies have revealed that human FcγRIIIa contains a substitution (Phe→Val) at residue 176 that can interact with IgG4 in addition to IgG1 and IgG3. Here we show that the squirrel monkey IgG interacts with its FcγRIIIa. Finally, highly sensitive and specific anti-squirrel monkey IgG monoclonal antibodies were developed for used in western blotting and ELISA assays.

Research Products

• [Journal Article] Endemic Burkitt lymphoma is associated with strength and diversity of Plasmodium falciparummalaria stage-specific antigen antibody response (2013)
  • Author(s): Aka P, Vila MC, Jariwala A, Nkrumah F, Emmanuel B, Yagi M, Palacpac NM, Periago MV, Neequaye J, Kiruthu C, Tougan T, Levine PH, Biggar RJ, Pfeiffer RM, Bhatia K, Horii T, Bethony JM, Mbulaiteye SM
  • Journal Title: Blood
  • DOI: doi:10.1182/blood-2012-12-475665
  • Peer Reviewed
• [Journal Article] Within-population genetic diversity of Plasmodium falciparum vaccine candidate antigens reveals geographic distance from a Central sub-Saharan African origin (2013)
  • Author(s): Tanabe K, Mita T, Palacpac NM, Arisue N, Tougan T, Kawai S, Jombart T, Kobayashi F, Horii T
  • Journal Title: Vaccine Volume: 31(9) Pages: 1334-9
  • DOI: doi:10.1016/j.vaccine.2012.12.039
  • Peer Reviewed
• [Journal Article] TLR9 adjuvants enhance immunogenicity and protective efficacy of the SE36/AHG malaria vaccine in nonhuman primate models (2013)
  • Author(s): Tougan T, Aoshi T, Coban C, Katakai Y, Kai C, Yasutomi Y, Ishii KJ, Horii T
  • Journal Title: Hum Vaccin Immunother Volume: 9(2) Pages: 283-290
  • DOI: doi:10.4161/hv.22950
  • Peer Reviewed
• [Journal Article] Plasmodium cynomolgi genome sequences provide insight into Plasmodium vivax and the monkey malaria clade (2012)
  • Author(s): Tachibana SI, Sullivan SA, Kawai S, Nakamura S, Kim HR, Goto N, Arisue N, Palacpac NM, Honma H, Yagi M, Tougan T, Katakai Y, Kaneko O, Mita T, Kita K, Yasutomi Y, Sutton PL, Shakhbatyan R, Horii T, Yasunaga T, Barnwell JW, Escalante AA, Ca
  • Journal Title: Nature Genetics Volume: 44(9) Pages: 1051-5
  • DOI: doi:10.1038/ng.2375
  • Peer Reviewed
• [Journal Article] Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza (2012)
  • Author(s): Matsuo K, Hirobe S, Yokota Y, Ayabe Y, Seto M, Quan YS, Kamiyama F, Tougan T, Horii T, Mukai Y, Okada N, Nakagawa S
  • Journal Title: J Control Release Volume: 160(3) Pages: 495-501
  • DOI: doi:10.1016/j.jconrel.2012.04.001
  • Peer Reviewed
• [Journal Article] Geographic differentiation of polymorphism in the Plasmodium falciparum malaria vaccine candidate gene SERA5 (2012)
  • Author(s): Tanabe K, Arisue N, Palacpac NM, Yagi M, Tougan T, Honma H, Ferreira MU, Farnert A, Bjorkman A, Kaneko A, Nakamura M, Hirayama K, Mita T, Horii T
  • Journal Title: Vaccine Volume: 30(9) Pages: 1583-93
  • DOI: doi:10.1016/j.vaccine.2011.12.124
  • Peer Reviewed
• [Journal Article] Antibodies reactive to Plasmodium falciparum serine repeat antigen in children with burkitt lymphoma from ghana (2012)
  • Author(s): Guech-Ongey M, Yagi M, Palacpac NM, Emmanuel B, Talisuna AO, Bhatia K, Cristina Stefan D, Biggar RJ, Nkrumah F, Neequaye J, Tougan T, Horii T, Mbulaiteye SM
  • Journal Title: Int J Cancer Volume: 130(8) Pages: 1908-14
  • DOI: doi:10.1002/ijc.26203
  • Peer Reviewed
• [Journal Article] The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe (2010)
  • Author(s): Tougan T, Kasama T, Ohtaka A, Okuzaki D, Saito TT, Russell P, Nojima H
  • Journal Title: Cell Cycle Volume: 9(23) Pages: 4688-4702
  • DOI: doi:10.4161/cc.9.23.14050
  • Peer Reviewed
• [Journal Article] GenopalTM: a novel hollow fiber array for focused microarray analysis (2010)
  • Author(s): Okuzaki D, Fukushima T, Tougan T, Ishii T, Kobayashi S, Akita T, Nojima H
  • Journal Title: DNA Research Volume: 17(6) Pages: 369-379
  • DOI: doi:10.1093/dnares/dsq025
  • Peer Reviewed
• [Journal Article] Evidences of protection against blood-stage infection of Plasmodium falciparum by the novel protein vaccine SE36 (2010)
  • Author(s): Horii T, Shirai H, Jie L, Ishii KJ, Palacpac NQ, Tougan T, Hato M, Ohta N, Bobogare A, Arakaki N, Matsumoto Y, Namazue J, Ishikawa T, Ueda S, Takahashi M
  • Journal Title: Parasitology Int Volume: 59(3) Pages: 380-386
  • DOI: doi:10.1016/j.parint.2010.05.002
  • Peer Reviewed
• [Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes (2010)
  • Author(s): Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ
  • Journal Title: Sci Transl Med Volume: 2(25) Pages: 25ra24
  • DOI: doi:10.1126/scitranslmed.3000759
  • Peer Reviewed
• [Presentation] 抗 SE36 抗体の誘導とマラリア抵抗性に関与する遺伝的バックグラウンドの研究/Analysis of African HLA genes in a population that participated in the Phase 1b trial of BK-SE36 (2013)
  • Author(s): 東岸任弘、Nirianne Q. Palacpac、八木正典、中村昇太、後藤和義、Thomas G. Egwang、堀井俊宏
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学湯島キャンパス(東京)
  • Year and Date: 20130329-31
• [Presentation] 新規アジュバントを添加した次世代SE36 マラリアワクチンの開発:旅行者用ワクチンへのアプローチ (2013)
  • Author(s): 東岸任弘、青枝大貴、Cevayir Coban、片貝祐子、甲斐知恵子、保富康宏、石井健、堀井俊宏
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20130327-30
• [Presentation] 抗 SE36 抗体の誘導とマラリア抵抗性に関与する遺伝的バックグラウンドの研究 (2013)
  • Author(s): 東岸任弘、Nirianne Q. Palacpac、八木正典、中村昇太、後藤和義、Thomas G. Egwang、堀井俊宏
  • Organizer: 感染症若手フォーラム 2013
  • Place of Presentation: 北広島クラッセホテル(北海道)
  • Year and Date: 20130228-0302
• [Presentation] リスザルは単一の IgG を持ち、サブクラスを持たない (2012)
  • Author(s): 東岸任弘、中村昇太、朴辰幸、片貝祐子、保富康宏、立花太郎、堀井俊宏
  • Organizer: 日本実験動物科学・技術 九州 2012
  • Place of Presentation: ビーコンプラザ(大分)
  • Year and Date: 20120524-26
• [Presentation] ホテル阪急エキスポパーク(大阪) (2012)
  • Author(s): 東岸任弘、中村昇太、朴辰幸、片貝祐子、保富康宏、立花太郎、堀井俊宏
  • Organizer: NGS 現場の会 第2回研究会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪)
  • Year and Date: 20120523-25
• [Presentation] Squirrel monkey has a single IgG and no IgG subclasses (2012)
  • Author(s): Takahiro Tougan, Shota Nakamura, Chin-Heng Paku, Yuko Katakai, Yasuhiro Yasutomi, Taro Tachibana and Toshihiro Horii
  • Organizer: 5th International CVRDC-RIMD Joint Symposium
  • Place of Presentation: SEOGWIPO KAL HOTEL(韓国・済州島)
  • Year and Date: 20120511-12
• [Presentation] ・リスザルは単一の IgG を持ち (2012)
  • Author(s): 東岸任弘、中村昇太、朴辰幸、片貝祐子、保富康宏、立花太郎、堀井俊宏
  • Organizer: 日本分子生物学会 第12回 春季シンポジウム(
  • Place of Presentation: 石和温泉・慶山(山梨)
  • Year and Date: 20120425-26
• [Presentation] リスザルは 1 種類の IgG しか持たず (2012)
  • Author(s): 東岸任弘、中村昇太、朴辰幸、片貝祐子、保富康宏、立花太郎、堀井俊宏
  • Organizer: 感染症若手フォーラム
  • Place of Presentation: 海の見えるホテル(長崎)
  • Year and Date: 20120202-04
• [Presentation] 新規アジュバント添加による次世代 SE36 マラリアワクチンの開発と実用化:旅行者用ワクチンへのアプローチ (2012)
  • Author(s): 東岸任弘、青枝大貴、八木正典、有末伸子、片貝祐子、保富康宏、甲斐知恵子、石井健、堀井俊宏
  • Organizer: 日本実験動物技術者協会北陸支部 本部共催講習会第331回
  • Place of Presentation: 金沢大学学際科学実験センター(石川)
  • Year and Date: 2012-03-10
• [Presentation] Squirrel monkey has a single IgG and no IgG subclasses (2011)
  • Author(s): Takahiro Tougan, Shota Nakamura, Chin-Heng Paku, Yuko Katakai, Yasuhiro Yasutomi, Taro Tachibana and Toshihiro Horii
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20111213-16
• [Presentation] Development of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine (2011)
  • Author(s): Takahiro Tougan, Taiki Aoshi, Masanori Yagi, Cevayir Coban, Nobuko Arisue, Yuko Katakai, Yasuhiro Yasutomi, Chieko Kai, Ken J. Ishii and Toshihiro Horii
  • Organizer: Nagasaki Symposium on Malaria Biology 2011 (II)
  • Place of Presentation: 長崎大学(長崎)
  • Year and Date: 20111116-17
• [Presentation] Development of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine (2011)
  • Author(s): Takahiro Tougan, Taiki Aoshi, Masanori Yagi, Cevayir Coban, Nobuko Arisue, Yuko Katakai, Yasuhiro Yasutomi, Chieko Kai, Ken J. Ishii and Toshihiro Horii
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Diseases
  • Place of Presentation: 奈良女子大学(奈良)
  • Year and Date: 20110912-16
• [Presentation] Development of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine. (2011)
  • Author(s): Takahiro Tougan, Taiki Aoshi, Masanori Yagi, Cevayir Coban, Nobuko Arisue, Yuko Katakai, Yasuhiro Yasutomi, Chieko Kai, Ken J. Ishii and Toshihiro Horii
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Congress
  • Place of Presentation: Sapporo Convention Center, Sapporo Business Innovation Center (北海道)
  • Year and Date: 20110906-10
• [Presentation] 新規アジュバント添加による次世代 SE36 マラリアワクチンの開発と実用化:旅行者用ワクチンへのアプローチ /Development and application of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine (2011)
  • Author(s): 東岸任弘、青枝大貴、八木正典、有末伸子、片貝祐子、保富康宏、甲斐知恵子、石井健、堀井俊宏
  • Organizer: 第7回霊長類医科学フォーラム
  • Place of Presentation: 研究交流センター 国際会議場(茨城)
  • Year and Date: 2011-11-18
• [Presentation] 新規アジュバント添加による次世代 SE36 マラリアワクチンの開発と実用化:旅行者用ワクチンへのアプローチ (2010)
  • Author(s): 東岸任弘・石井健・堀井俊宏
  • Organizer: 第14回日本ワクチン学会学術集会
  • Place of Presentation: 九段会館(東京)
  • Year and Date: 20101211-12
• [Presentation] 新規アジュバント添加による次世代SE36 マラリアワクチンの開発と実用化:旅行者用ワクチンへのアプローチ/Development and application of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine. (2010)
  • Author(s): 東岸任弘・青枝大貴・八木正典・有末伸子・片貝祐子・保富康宏・甲斐知恵子・石井健・堀井俊宏
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 新規アジュバント添加による次世代SE36 マラリアワクチンの開発と実用化:旅行者用ワクチンへのアプローチ/Development and application of next generation SE36 malaria vaccine formulated with a novel adjuvant: approach to travelers' vaccine.
  • Year and Date: 20100520-21
• [Book] Vaccine (2011)
  • Author(s): Palacpac NM, Arisue N, Tougan T, Ishii KJ, Horii T
  • Total Pages: 5837-45
• [Book] Methods Mol Biol (2011)
  • Author(s): Nojima H, Tougan T
  • Total Pages: 15-35
• [Book] 綜合臨牀 (2011)
  • Author(s): 東岸任弘、堀井俊宏
  • Total Pages: 53-57
• [Book] Pharma Medica (2011)
  • Author(s): 東岸任弘、田邊和裄、堀井俊宏
  • Total Pages: 53-57
• [Book] 臨床粘膜免疫学 (2010)
  • Author(s): 東岸任弘、堀井俊宏
  • Total Pages: 489-497
  • Publisher: 株式会社シナジー
• [Book] 臨床検査 (2010)
  • Author(s): 東岸任弘、堀井俊宏
  • Total Pages: 1413-1421
• [Book] 東次世代ワクチンの産業応用技術 (2010)
  • Author(s): 東岸任弘、石井健、堀井俊宏
  • Total Pages: 175-186
• [Book] 新薬展望 (2010)
  • Author(s): 東岸任弘、堀井俊宏
  • Total Pages: 139(349)-145(355)
• [Book] DDS(DDS 学会誌) (2010)
  • Author(s): 東岸任弘、石井 健、堀井俊宏
  • Total Pages: 37-45
• [Remarks] 大阪大学・微生物病研究所・分子原虫学分野ホームページ
  • URL: http://www.biken.osaka-u.ac.jp/lab/protozool/index.html