Analysis of a novel histone modification associated with the UHRF1 complex

2011 Fiscal Year Final Research Report

• Project/Area Number: 22700867
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Carcinogenesis
• Research Institution: Kyushu University
• Principal Investigator: UNOKI Motoko 九州大学, 生体防御医学研究所, 助教 (30525374)
• Project Period (FY): 2010 – 2011
• Keywords: UHRF1 / JMJD6 / エピジェネティクス / ヒストン修飾 / 水酸化

Research Abstract

We identified a hydroxylase JMJD6 as a member of the UHRF1 complex, which works around nucleosomes, and found that JMJD6 hydroxylates histone lysine residues in vitro. We also revealed that histones purified from Jmjd6 wild-type mouse embryos include 5-hydroxylysine, but those purified from Jmjd6 knockout mouse embryos do not include it, indicating that Jmjd6 is a hydroxylase, which hydroxylates lysine residues of histones in vivo.

Research Products

• [Journal Article] NIRF/UHRF2 occupies a central position in the cell cycle network and allows coupling with the epigenetic landscape (2012)
  • Author(s): Mori T, Ikeda DD, Yamaguchi Y, Unoki M & NIRF Project
  • Journal Title: FEBS Lett Volume: (in press)
  • Peer Reviewed
• [Journal Article] Current and Potential Anticancer Drugs Targeting Members of the UHRF1 Complex Including Epigenetic Modifiers. (2011)
  • Author(s): Unoki M
  • Journal Title: Recent Pat. Anticancer Drug Discov Volume: 6 Pages: 116-130
  • Peer Reviewed
• [Journal Article] Demethylation of RB regulator MYPTl by histone demethylase LSDl promotes cell cycle progression in cancer cells (2011)
  • Author(s): Che HS, Suzuki T, Dohmae N, Hayami S, Unoki M, Yoshimatsu M, Toyokawa G, Takawa M, Chen T, Kurash JK, Field HI, Ponder BA, Nakamura Y & Hamamoto R
  • Journal Title: Cancer Res Volume: 71 Pages: 655-660
  • Peer Reviewed
• [Journal Article] Dysregulat ion of PRMTl and PRMT6, type I arginine methyltransferases, is involved in various types of human cancers (2011)
  • Author(s): Yoshimatsu M, Toyokawa G, Hayami S, Unoki M, Tsunoda T, Field HI, Kelly JD, Neal DE, Maehara Y, Ponder BAJ, Nakamura Y & Hamamoto R
  • Journal Title: In t. J. Can Volume: 128 Pages: 562-563
  • Peer Reviewed
• [Journal Article] Overexpression of LSDl contributes to human carcinogenesis through chromatin regulation in various cancers (2011)
  • Author(s): Hayami S, Kelly JD, Cho HS, Yoshimatsu M, Unoki M, Tsunoda T, Field HI, Yamaue H, Ponder BAJ, Nakamura Y & Hamamoto R
  • Journal Title: Int. J. Can Volume: 128 Pages: 574-586
  • Peer Reviewed
• [Journal Article] UHRFl is a novel diagnostic marker of lung cancer (2010)
  • Author(s): Unoki M, Daigo Y, Koinuma J, Tsuchiya E, Hamamoto R & Nakamura Y
  • Journal Title: Br. J. Cancer Volume: 103 Pages: 217-222
  • Peer Reviewed
• [Journal Article] Overexpression of the JmjC hi stone demethylase KDMsB in human carcinogenesis : Involvement in the proliferation of cancer cells through the E2F/RB pathway (2010)
  • Author(s): Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BAJ, Nakamura Y & Hamamoto R
  • Journal Title: Mol. Cancer Volume: 9, 59
  • Peer Reviewed
• [Presentation] In vivoにおけるヒストン水酸化酵素としてのJMJD6の同定 (2012)
  • Author(s): 鵜木元香、益田晶子、堂前直、福井宣規、有田恭平、浜本隆二、岩井裕希子、白川昌宏、佐々木裕之、中村祐輔
  • Organizer: 第6回エピジェネティクス研究会年会
  • Place of Presentation: 東京一ツ橋学術総合センター
  • Year and Date: 20120514-15
• [Presentation] BUHRFl複合体に含まれるJMJD6によるヒストンリジン残基の水酸化について。 (2011)
  • Author(s): 鵜木元香. 、吉松正憲、有田恭平、益田晶子、堂前直、植田幸嗣、岩井裕希子、醍醐弥太郎、浜本隆二、白川昌宏、中村祐輔
  • Organizer: 第5回エピジェネティクス研究会年会。
  • Place of Presentation: KKR熊本ホテル
  • Year and Date: 20110519-20
• [Book] BLADDER CANCER,-from basic science to robotic surgery-(C hapter 6 : UHRFl1 is a potential mole cular marker for diagnosis and prog no siss of bladder cancer.) (2012)
  • Author(s): Unoki M
  • Total Pages: 129-146
  • Publisher: InTech
• [Book] 発生におけるエピジェネティクスとその破綻 (2012)
  • Author(s): 鵜木元香 、佐々木裕之
  • Total Pages: 2-8
  • Publisher: SIGMA