Establishment of Glioma stem-like cells(GSC) from glioma patient tumors and analysis of the novel molecular markers of GSC by an integrated proteomics.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22700905
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Tumor diagnosis
• Research Institution: Kumamoto University
• Principal Investigator: NANBU Akiko 熊本大学, 大学院・生命科学研究部, 産学連携研究員 (40572087)
• Research Collaborator: ARAKI Norie 熊本大学, 大学院・生命科学研究部, 准教授 (80253722) ; MIZUGUCHI Souhei 熊本大学, 大学院・生命科学研究部, 産学連携博士研究員 (50398103) ; MIDORIKAWA Uichi 熊本大学, 大学院・医学教育部, 博士
• Project Period (FY): 2010 – 2011
• Keywords: プロテオミクス解析 / がん幹細胞

Research Abstract

Glioma stem cells(GSCs) are thought to be responsible for the therapeutic resistance and recurrence of malignant glioma. The molecules regulating the maintenance/differentiation of these cells are considered effective therapeutic targets. In this study, we established GSC clones from tumors of malignant glioma patients and subjected them to integrated proteomics to survey the proteins and mRNAs differentially expressed in GSCs after they have been induced by serum stimulation. This resulted in the identification of 30000 molecules and GO analysis revealed that the expression of cell adhesion molecules, including integrin subfamily members and extracellular matrices(ECMs) was significantly upregulated during GSC differentiation. After the cell-biological validations, we found that serum factor-induced coupling of ECMs to differentiation and proliferation. The results raised the possibility that GSC induces/secretes ECMs by itself to form a specific microenvironment, called the "differention niche", which facilitates the development of malignant glioma.

Research Products

• [Presentation] 融合プロテオミクスによる幹細胞様グリオーマの分化制御機構の解析 (2011)
  • Author(s): 南部晶子, 緑川宇一, 水口惣平, 長山慈, 永井美奈子, 平野久, 荒木令江
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20110921-24
• [Presentation] プロテオミクスによるグリオーマ幹細胞分化に関する特異的分子群のプロファイリング解析 (2010)
  • Author(s): 緑川宇一, 新堀晶子, 水口惣平, 新森加奈子, 丸尾裕二, 鵜沼豊, 中村眞, 荒木令江
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] プロテオミクスによるグリオーマ幹細胞分化に関する特異的分子群のプロファイリング解析 (2010)
  • Author(s): 緑川宇一, 新堀晶子, 水口惣平, 新森加奈子, 丸尾裕二, 鵜沼豊, 中村眞, 荒木令江
  • Organizer: 第33回分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] Integrated proteomics of cancer cellular activation signals related to chemotherapy resistances (2010)
  • Author(s): S. Mizuguchi, T. Morikawa, N. Tsubota, U Midorikawa, A. Niibori, D. Kobayashi, H. Nakamura, J. Kuratsu, N. Araki
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] The functional study of vimentin network involved in chemo sensitivity of gliomas using integrated proteomics data processing/mining approach (2010)
  • Author(s): S. Mizuguchi, T. Morikawa, N. Tsubota, U Midorikawa, M. Nagayama, D. Kobayashi, A. Niibori, A. Wilson, M. Wilson Morifuji, H. Nakamura, J. Kuratsu, N. Araki
  • Organizer: The Human Proteome Organization(HUPO) 9th Annual World Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 20100919-23