2011 Fiscal Year Final Research Report
Development of the on chip microchamber array device for single cell gene analysis
| Project/Area Number |
22710122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Osaka University |
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Principal Investigator |
SAITO Masato 大阪大学, 大学院・工学研究科, 助教 (80457001)
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| Project Period (FY) |
2010 – 2011
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| Keywords | シングルセル / PCR / RT-PCR / マイクロチャンバー |
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| Research Abstract |
For analyzing a single cell contents or gene information, we have fabricated the microchamber array chip which has microscale reaction fields. One of chip substrate material was silicon and other one was polystylen polymer. After evaluation and optimization of the on-chip PCR conditions, DNA amplification from a single cell in the microchamber, was attempted. A single cells were placed in each microchambers by micromanipulating. As a result, we could successfully observed the fluorescence which was associated with DNA amplification from a single cells.
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Research Products
(15 results)
