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2011 Fiscal Year Final Research Report

Characterization of sugar-sugar glycosyltransferases involved in plant secondary metabolism.

Research Project

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Project/Area Number 22710218
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Living organism molecular science
Research InstitutionNagoya City University

Principal Investigator

TERASAKA Kazuyoshi  名古屋市立大学, 大学院・薬学研究科, 助教 (60405214)

Project Period (FY) 2010 – 2011
Keywords植物二次代謝 / 低分子有機化合物配糖体 / 糖転移酵素
Research Abstract

We isolated cDNA clones encoding novel sugar-sugar glucosyltransferases catalyzing 1, 6-glucosylation from Gardenia jasminoides and Glehnia littoralis that produce gentiobioside compounds in planta. These recombinant proteins exhibited a unique glucosyl chain elongation activity forming gentiobioside.

  • Research Products

    (6 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides2012

    • Author(s)
      Mai Nagatoshi, Kazuyoshi Terasaka, Miki Owaki, Makiko Sota, Tatsunori Inukai, Akito Nagatsu, Hajime Mizukami
    • Journal Title

      FEBS Letters

      Volume: 586巻 Pages: 1055-1061

    • DOI

      DOI:10.1016/j.febslet.2012.03.003

    • Peer Reviewed
  • [Journal Article] Iridoid-specific Glucosyltransferase from Gardenia jasminoides2011

    • Author(s)
      Mai Nagatoshi, Kazuyoshi Terasaka, Akito Nagatsu, Hajime Mizukami
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286巻 Pages: 32866-32874

    • DOI

      DOI:10.1074/jbc.M111.242586

    • Peer Reviewed
  • [Presentation] 組換え酵素カクテルを用いたzeaxanthinからcrocinの生産2012

    • Author(s)
      永利麻衣
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-29
  • [Presentation] ハマボウフウ培養細胞由来クマリン配糖化酵素の機能解析2011

    • Author(s)
      岡本隼己
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-09-08
  • [Presentation] ハマボウフウ培養細胞由来coumarin配糖化酵素のクローニングと機能解析2011

    • Author(s)
      岡本隼己
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡市)
    • Year and Date
      2011-03-31
  • [Presentation] クチナシ培養細胞由来crocetin配糖化酵素の単離と機能解析2010

    • Author(s)
      寺坂和祥
    • Organizer
      第28回日本植物細胞分子生物学会(仙台)大会
    • Place of Presentation
      東北大学雨宮キャンパス(仙台市)
    • Year and Date
      2010-09-03

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Published: 2013-07-31  

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