2011 Fiscal Year Final Research Report
Real-time monitoring of variable viscoelasticity of proteins during translation reaction
| Project/Area Number |
22750147
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TAKAHASHI Shuntaro 東京工業大学, 大学院・生命理工学研究科, 特任助教 (40456257)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 翻訳 / リボソーム / フォールディング / 水晶発振子 / リアルタイムモニタリング / 粘弾性 |
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| Research Abstract |
We used a quartz crystal microbalance technique to monitor the rate of mRNA translation in real time. This method permitted us to evaluate the translation of proteins of interest fused upstream of a streptavidin-binding peptide fusion protein. The results indicated that the rate of translation can be regulated by not only the sequence of mRNA, but also the protein translated. We will be able to understand how the property of the protein translated affects the reaction of translation by evaluating the viscoelasticity of the protein during translation together with monitoring the rate of translation.
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