2011 Fiscal Year Final Research Report
Development of measurement of intracellular ion concentration using a phase-resolved fluorescence lifetime method for multicomponent
| Project/Area Number |
22760036
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied optics/Quantum optical engineering
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| Research Institution | Shizuoka University |
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Principal Investigator |
INAMI Wataru 静岡大学, 若手グローバル研究リーダー育成拠点, 特任助教 (30542815)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 光計測 / 蛍光寿命 / 位相分解蛍光寿命測定法 / カルシウムイオン |
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| Research Abstract |
Calcium ion concentration was measured with phase-resolved fluorescence lifetime method. Fluorescence dye Calcium Orange was used. The fluorescence lifetime was changed when the concentration of calcium ion was varied from10 to 2000nM. In order to improve the quantitativity of the calcium ion measurement, we investigated a fluorescence lifetime of calcium orange in calcium ion solution including protein. As a result, the fluorescence lifetime does not depend on the protein concentration range from 40 to 80 mg/ml, which is same as that in a cell.
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