High-throughput analysis and engineering of fungi transcription factor binding sequences.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22760605
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: KOJIMA Takaaki 名古屋大学, 生命農学研究科, 助教 (40509080)
• Project Period (FY): 2010 – 2012
• Keywords: 転写因子 / プロモーター活性 / エマルジョンPCR / 糸状菌 / ハイスループットスクリーニング

Research Abstract

Bead display is a novel library construction method in which clonal DNA moleculesare displayed on a large population of microbeads. Comprehensive analysis of DNA binding regions of a fungal transcription factor, AmyR, was carried out with a random DNA library or A. nidulans genome library by using bead display. As the results, the extraction of AmyR binding motifs and the selection of various binding sites of AmyR were observed. In addition, a high-throughput screening method for promoter activity was developed by using bead display and a ligase ribozyme

Research Products

• [Journal Article] High-throughput screening method for promoter activity using bead display and a ligase ribozyme (2012)
  • Author(s): Kojima, T., Ohuchi, S., Ito, Y., Nakano, H.
  • Journal Title: J. Biosci Bioeng. Volume: 114 Pages: 671-676
  • URL: http://dx.doi.org/10.1016/j.jbiosc.2012.06.011
  • Peer Reviewed
• [Journal Article] Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, by using a Bead Display System (2011)
  • Author(s): Wang, P., Kojima, T., Kobayashi, T., and Nakano, H
  • Journal Title: Biotechnol. Biochem Volume: 76 Pages: 1128-1134
  • URL: http://dx.doi.org/10.1271/bbb.110949
  • Peer Reviewed
• [Presentation] ビーズディスプレイ法を用いたin vitroプロモーターハイスループットスクリーニング法の確立 (2012)
  • Author(s): 伊藤祐里恵、兒島孝明、大内将司、中野秀雄
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 20121000
• [Presentation] ビーズディスプレイ法を用いたDNA-糸状菌転写因子相互作用検出システム (2012)
  • Author(s): 江崎俊文、王ハン輝、兒島孝明、中野秀雄
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 20121000
• [Presentation] Comprehensive analysis of transcription factor binding site using bead display of DNA (2012)
  • Author(s): Nakano, H.,Wang, P.W. and Kojima, T
  • Organizer: The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Kanazawa
  • Year and Date: 20120500
• [Presentation] In vitro high-throughput promoter screening system using a bead display and a class I ligase ribozyme (2012)
  • Author(s): Ito, Y., Kojima, T., Ohuchi, S. and Nakano, H
  • Organizer: The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Kanazawa
  • Year and Date: 20120500
• [Presentation] ビーズディスプレイ法とクラスI リガーゼ・リボザイムを用いたハイスループットなin vitro スクリーニング法の確立 (2012)
  • Author(s): 伊藤祐里恵、兒島孝明、大内将司、中野秀雄
  • Organizer: 日本農芸化学会2012大会
  • Place of Presentation: 京都
  • Year and Date: 20120300
• [Presentation] ビーズディスプレイを用いたDNA-転写因子相互作用検出システムに関する研究 (2012)
  • Author(s): 王ハン暉、兒島孝明、中野秀雄
  • Organizer: 日本農芸化学会2012大会
  • Place of Presentation: 京都
  • Year and Date: 20120300
• [Presentation] エマルジョンPCRを用いた分子間相互作用ハイスループットスクリーニング系 (2011)
  • Author(s): 中野秀雄、兒島孝明
  • Organizer: 平成23年度生物工学技術セミナー企画
  • Place of Presentation: 東京
  • Year and Date: 20111100
• [Presentation] Novel screening method for promoter activity using a bead display and a ligase ribozyme (2011)
  • Author(s): Kojima, T., Ito, Y., Ohuchi, S. and Nakano, H.
  • Organizer: 第5回GCOEリトリート
  • Place of Presentation: 長浜
  • Year and Date: 20110900
• [Presentation] Detections of DNA-Transcription Factor Interactions using a Microbeads Display System (2011)
  • Author(s): Wang, P., Kojima, T. and Nakano, H
  • Organizer: 第5回GCOEリトリート
  • Place of Presentation: 長浜
  • Year and Date: 20110900
• [Presentation] ビーズディスプレイ法とクラスIリガーゼ・リボザイムを用いたプロモーター活性in vitroハイスループットスクリーニング (2011)
  • Author(s): 兒島孝明、伊藤祐里恵、大内将司、中野秀雄
  • Organizer: 日本生物工学会2011年63回大会
  • Place of Presentation: 小金井
  • Year and Date: 20110900
• [Presentation] ビーズディスプレイ法とリガーゼ・リボザイムを用いたプロモーター活性in vitro 選別法 (2011)
  • Author(s): 兒島孝明、伊藤祐里恵、大内将司、中野秀雄
  • Organizer: 生物工学若手研究者の集い 夏のセミナー2011
  • Place of Presentation: 笛吹
  • Year and Date: 20110700
• [Presentation] DNAのビーズディスプレイ法を用いたDNA-糸状菌転写因子相互作用のハイスループット検出法 (2011)
  • Author(s): 王 ハン輝、児島 孝明、中野 秀雄
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都→震災の為発表中止
  • Year and Date: 20110300
• [Presentation] Beads display:A Novel High-throughput Screening Technology to Explore Functional Biomolecules (2010)
  • Author(s): Kojima, T. and Nakano, H
  • Organizer: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Chengdu, China
  • Year and Date: 20101100
• [Presentation] 無細胞蛋白質合成系を用いた1分子・1細胞由来mRNAの解析技術。 (2010)
  • Author(s): 中野秀雄、兒島孝明
  • Organizer: 日本生物工学会
  • Place of Presentation: 宮崎
  • Year and Date: 20101000
• [Presentation] ビーズディスプレイ法とリガーゼ・リボザイムを用いたプロモーター活性選別法の開発 (2010)
  • Author(s): 兒島孝明、大内将司、中野秀雄
  • Organizer: 日本生物工学会2010年62回大会
  • Place of Presentation: 宮崎
  • Year and Date: 20101000
• [Presentation] High-throughput Screening of DNA Binding Sites for Fungus Transcription Factor, AmyR, Using Beads Display System of DNA (2010)
  • Author(s): Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. and Nakano, H
  • Organizer: グローバルCOE第4回リトリート
  • Place of Presentation: 豊橋
  • Year and Date: 20100900
• [Presentation] etections of DNA-transcription factor interactions using a Microbeads Display System (2010)
  • Author(s): Wang, P., Kojima T. and Nakano, H
  • Organizer: グローバルCOE第4回リトリート
  • Place of Presentation: 豊橋
  • Year and Date: 20100900