Chromatin structure and nuclear territories of the intergenic region between inactivated and escape genes on human inactive X chromosome.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22770008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Chubu University
• Principal Investigator: GOTO Yuji 中部大学, 生命健康科学部, 助手 (70362522)
• Project Period (FY): 2010 – 2012
• Keywords: ゲノム機能 / 発現

Research Abstract

In this study, I clarified the structure and mechanism of chromatin boundary between the inactivated KLHL15 and an escape gene, EIF2S3 by using human-mouse hybrid cells carrying either active or inactive human X chromosome in mouse background. Main findings are as follows;1) Three matrix attachment regions (MAR) located between KLHL15 and EIF2S3 in the inactive X-chromosome have been exclusively associated to nuclear matrix.2) Acetylation of histone H3K9 and methylation of histone H3K4 have been enriched at the escape gene, EIF2S3, and their flanking region. Tri-methylation of histone H3K27 and H3K9, on the other hand, have been enriched at the inactivated, KLHL15. These modifications were separated at MAR.3) Knockdown of SATB1, which is the member of MAR associated protein complex, led to the disruption of MAR and nuclear matrix pairing and histone modification.

Research Products

• [Journal Article] c-Ret-mediated hearing losses. (2012)
  • Author(s): Ohgami N, Tamura H, Ohgami K, Iida M, Yajima I, Kumasaka MY, Goto Y, Sone M, Nakashima T, and Kato M.
  • Journal Title: International Journal of Clinical and Experimental Pathology. Volume: 5 Pages: 23-8
  • Peer Reviewed
• [Journal Article] RAS/RAF/MEK/ERK and PI3K/PTEN/AKT (2012)
  • Author(s): Yajima I, Kumasaka MY, Thang ND, Goto Y, Takeda K, Yamanoshita O, Iida M, Ohgami N, Tamura H, Kawamoto Y, and Kato M.
  • Journal Title: Signaling in Malignant Melanoma Progression and Therapy.
  • Peer Reviewed
• [Journal Article] Molecular network associated with MITF in skin melanoma development and progression. (2011)
  • Author(s): Yajima I, Kumasaka MY, Thang ND, Goto Y,Takeda K, Iida M, Ohgami N, Tamura H, Yamanoshita O, Kawamoto Y, Furukawa K, and Kato M.
  • Journal Title: Journal of skin cancer.
  • DOI: doi:10.1155-2011-730170.
  • Peer Reviewed
• [Journal Article] Antioxidative effects of cherry leaves extract on tert-butyl hydroperoxide-mediated cytotoxicity through regulation of thioredoxin-2 protein expression levels. (2011)
  • Author(s): Taguchi N, Uemura N, Goto Y, Sakura M, Hara K, Niwa M, Iida M, Yanagishita T,Watanabe D, and Kato M.
  • Journal Title: J Toxicology and Environmental Health PartA. Volume: 74 Pages: 1240-1247
  • Peer Reviewed
• [Journal Article] Sunlight exposure-mediated DNA damage in young adults. (2011)
  • Author(s): Kato M, Iida M, Goto Y, Kondo T, andYajima I.
  • Journal Title: Cancer Epidemiology and BiomarkersPreview. Volume: 20 Pages: 1622-1688
  • Peer Reviewed
• [Journal Article] L-cysteine as a regulator for arsenic-mediated cancer-promoting and anti-cancer effects. (2011)
  • Author(s): Kato M, Kumasaka MY, Takeda K, HossainK, Iida M, Yajima I, Goto Y, and Ohgami N.
  • Journal Title: Toxicology In Vitro. Volume: 25 Pages: 623-629
  • Peer Reviewed
• [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマチン構造 (2013)
  • Author(s): 後藤友二
  • Organizer: 哺乳動物X染色体研究会
  • Place of Presentation: 北大、札幌
  • Year and Date: 2013-03-23
• [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマ チン構造と転写制御 (2012)
  • Author(s): 後藤友二
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九大、福岡
  • Year and Date: 2012-09-25
• [Presentation] 不活性化を免れる遺伝子 EIF2S3 上流にあるクロマチン構造の解析 II (2011)
  • Author(s): 後藤友二 、加藤昌志
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京大、京都
  • Year and Date: 2011-09-20
• [Presentation] メラノーマ自然発症モデルマウスを用いた癌抑制遺伝子のスクリーニングと機能解析 (2010)
  • Author(s): 後藤友二 、加藤昌志
  • Organizer: 日本分子生物学会第33回大会
  • Place of Presentation: ポートアイランド、神戸
  • Year and Date: 2010-12-08
• [Presentation] 不活性化を免れる遺伝子 EIF2S3 上流にあるクロマチン構造の解析 (2010)
  • Author(s): 後藤友二 、加藤昌志
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 北大、札幌
  • Year and Date: 2010-09-20