2011 Fiscal Year Final Research Report
Very long chain ceramide biosynthesis and its molecular mechanism
| Project/Area Number |
22770095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University (2011)
Hokkaido University (2010) |
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Principal Investigator |
MIZUTANI Yukiko 名古屋大学, 医学系研究科, COE 特任助教 (30396296)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 脂質 / セラミド / スフィンゴ脂質 / 極長鎖脂肪酸 / 酵素 / バリア機能 |
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| Research Abstract |
The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide, comprising an ultra long-chain fatty acid of >=26 carbons, plays an essential role in barrier formation. Ultra long-chain acyl-CoAs, produced by the FA elongase (ELOVL), are converted to ultra long-chain ceramides by the ceramide synthase (CERS). In the keratinocyte differentiation system, we observed that these mRNAs and proteins expression increased. We also determined that peroxisome proliferator-activated receptor is involved in the up-regulation of the mRNAs. Knockdown of CERS caused a reduction in the elongase activities toward ultra long-chain acyl-CoAs. These results are implying the possibility that ceramide synthasepositively regulates ultra long-chain fatty acid production. We also examined the possibility that the PPAR activation of ceramide can regulate the production of ceramides.
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