2011 Fiscal Year Final Research Report
Structure of photoreceptor membrane protein by In-situ photoirradiated solid-state NMR
Project/Area Number |
22770101
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Yokohama National University |
Principal Investigator |
KAWAMURA Izuru 横浜国立大学, 工学研究院, 助教 (20452047)
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Co-Investigator(Renkei-kenkyūsha) |
NAITO Akira 横浜国立大学, 工学研究院, 教授 (80172245)
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Project Period (FY) |
2010 – 2011
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Keywords | 固体NMR / 膜タンパク質 / 構造生命科学 / バクテリオロドプシン / 光中間体 / レチナール / センサリーロドプシン / 細胞膜 |
Research Abstract |
In situ photoirradiated solid-state NMR have been developed and demonstrated to successfully observe the M-photointermediate of pharaonis phoborhodopsin(ppR) and T204A mutant in the lipid environment. The C^<13> NMR signals of[20-C^<13>] retinal-ppR with transducer revealed that multiple M-photointermediates with 13-cis, 15-anti retinal configuration under continuously photoirradiated condition. Our results provide insights into the process of photocycle in the ppR with transducer protein.
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[Journal Article] An Active Photoreceptor Intermediate Revealed by In Situ Photoirradiated Solid-State NMR Spectroscopy2011
Author(s)
Y. Tomonaga, T. Hidaka, I. Kawamura, T. Nishio, K. Ohsawa, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, A. Ramamoorthy, A. Naito
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Journal Title
Biophys. J.
Volume: 101
Pages: L50-L52
Peer Reviewed
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