Structure of photoreceptor membrane protein by In-situ photoirradiated solid-state NMR

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Yokohama National University
• Principal Investigator: KAWAMURA Izuru 横浜国立大学, 工学研究院, 助教 (20452047)
• Co-Investigator(Renkei-kenkyūsha): NAITO Akira 横浜国立大学, 工学研究院, 教授 (80172245)
• Project Period (FY): 2010 – 2011
• Keywords: 固体NMR / 膜タンパク質 / 構造生命科学 / バクテリオロドプシン / 光中間体 / レチナール / センサリーロドプシン / 細胞膜

Research Abstract

In situ photoirradiated solid-state NMR have been developed and demonstrated to successfully observe the M-photointermediate of pharaonis phoborhodopsin(ppR) and T204A mutant in the lipid environment. The C^<13> NMR signals of[20-C^<13>] retinal-ppR with transducer revealed that multiple M-photointermediates with 13-cis, 15-anti retinal configuration under continuously photoirradiated condition. Our results provide insights into the process of photocycle in the ppR with transducer protein.

Research Products

• [Journal Article] Local Dynamics Change of Bacteriorhodopsin with Retinal Isomerization under Pressure as studied by Fast Magic Angle Spinning NMR (2012)
  • Author(s): I. Kawamura, S. Yamaguchi, H. Nishikawa, K. Tajima, M. Horigome, S. Tuzi, H. Saito, A. Naito
  • Journal Title: Polym. J. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] 動的核分極DNPによる超高感度固体NMR法の生体分子への展開 (2012)
  • Author(s): 川村出、内藤晶
  • Journal Title: 分光研究 Volume: 61巻
  • Peer Reviewed
• [Journal Article] An Active Photoreceptor Intermediate Revealed by In Situ Photoirradiated Solid-State NMR Spectroscopy (2011)
  • Author(s): Y. Tomonaga, T. Hidaka, I. Kawamura, T. Nishio, K. Ohsawa, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, A. Ramamoorthy, A. Naito
  • Journal Title: Biophys. J. Volume: 101 Pages: L50-L52
  • Peer Reviewed
• [Journal Article] Site-Specific Solid-State NMR Detection of Hydrogen-Deuterium Exchange Reveals Conformational Changes in a 7-Helical Transmembrane Protein (2011)
  • Author(s): S. Wang, L. Shi, I. Kawamura, L. S. Brown, V. Ladizhansky
  • Journal Title: Biophys. J. Volume: 101 Pages: L23-L25
  • Peer Reviewed
• [Journal Article] Conformation of a Seven Helical Transmembrane Photosensor in the Lipid Environment (2011)
  • Author(s): L. Shi, I. Kawamura, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Journal Title: Angew. Chem. Int. Volume: 50 Pages: 1302-1305
  • Peer Reviewed
• [Presentation] Photo-induced dynamics change of Phoborhodopsin with transducer studied by C^<13> solid-state NMR (2011)
  • Author(s): I. Kawamura, R. Furusato, T. Hidaka, T. Okitsu, A. Wada, N. Kamo, A. Naito
  • Organizer: ISNMR2011
  • Place of Presentation: Yokohama Japan
  • Year and Date: 20111115-19
• [Presentation] Dynamics change of phoborhodopsin with its cognate transducer protein as studied by in-situ photo-irradiated solid-state NMR (2011)
  • Author(s): I. Kawamura, R. Furusato, T. Kondo, T. Okitsu, A. Wada, N. Kamo, A. Naito
  • Organizer: The 4th Asia-Pacific NMR symposium
  • Place of Presentation: Beijing, China
  • Year and Date: 20111016-19
• [Presentation] Photo-induced dynamics change of phoborhodopsin with transducer protein as studied by in-situ phoro irradiated solid-state NMR (2011)
  • Author(s): 川村出、古里龍太郎、柴藤祐介、日高徹郎、沖津貴志、和田昭盛、加茂直樹、内藤晶
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス、姫路
  • Year and Date: 20110916-18
• [Presentation] 固体NMRによるアミロイド.膜タンパク質の構造解析 (2011)
  • Author(s): 川村出
  • Organizer: 第12回若手NMR研究会
  • Place of Presentation: 琵琶湖リゾートクラブ、滋賀
  • Year and Date: 20110622-24
• [Presentation] 固体NMRによる脂質膜中の7本膜貫通型-光受容膜タンパク質の構造解析 (2011)
  • Author(s): 川村出, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク、大阪
  • Year and Date: 20110607-09
• [Presentation] 固体NMRによる7本膜貫通型-光受容膜タンパク質の構造解析 (2011)
  • Author(s): 川村出, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 日本化学会第91回春季年会
  • Place of Presentation: 奈川大学横浜キャンパス
  • Year and Date: 20110326-29
• [Presentation] 固体NMRによる脂質二重膜中の7本膜貫通型-光受容膜タンパク質の構造解析 (2011)
  • Author(s): 川村出, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] 固体NMRによる光受容膜タンパク質の構造解析 (2011)
  • Author(s): 川村出
  • Organizer: 奈良先端大・青学大・横浜国大光応答分子材料に関する3大学共同セミナー
  • Place of Presentation: 横浜国立大学中央図書館メディアホール、横浜
  • Year and Date: 2011-07-28
• [Presentation] 固体NMRによるアナベナセンサリーロドプシンの構造解析 (2010)
  • Author(s): 川村出, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 第49回NMR討論会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 20101115-17
• [Presentation] Solid-state NMR structural study of anabaena sensory rhodopsin in the lipid environment (2010)
  • Author(s): I. Kawamura, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 3rd Japanese-French Joint Seminar on Organic Photochromism
  • Place of Presentation: Nisseki Yokohama Hall, Yokohama, Japan
  • Year and Date: 20101021-22
• [Presentation] Three-dimensional solid-state NMR study of Anabaena Sensory Rhodopsin in the lipid environment. Chemical shift assignments- (2010)
  • Author(s): 川村出, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内キャンパス、仙台
  • Year and Date: 20100920-22
• [Presentation] Structural change of Tyr residues in Bacteriorhodopsin corresponding to retinal configurations as studied by solid-state NMR (2010)
  • Author(s): I. Kawamura, M. Horigome, S. Tuzi, A. Naito
  • Organizer: 14th International Conference on Retinal Proteins(ICRP2010)
  • Place of Presentation: UCSC, Santa Cruz, San Francisco
  • Year and Date: 20100802-06
• [Presentation] Protein backbone conformations of archaeal-type rhodopsins as studied by solid-state NMR (2010)
  • Author(s): I. Kawamura, M. Horigome, S. Tuzi, A. Naito
  • Organizer: Pacifichem2010
  • Place of Presentation: Hawaii, Honolulu
  • Year and Date: 2010-12-16
• [Book] 広がる核磁気共鳴NMRの世界 (2011)
  • Author(s): 川村出
  • Total Pages: 78-81
  • Publisher: コロナ社
• [Remarks] 横浜国立大学研究者総覧
  • URL: http://er-web.jmk.ynu.ac.jp/