Structural study of translesion DNA polymeraseζ/REV1 complex

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770109
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: HASHIMOTO Hiroshi 横浜市立大学, 生命ナノシステム科学研究科, 助教 (40336590)
• Project Period (FY): 2010 – 2011
• Keywords: X線結晶解析

Research Abstract

REV1, REV3 and REV7 are pivotal proteins in translesion DNA synthesis that allows to continue DNA synthesis even in the presence of DNA damage. REV1 and REV3 are error-prone DNA polymerases. REV7 interacts with both polymerases, thereby acts as an adaptor protein that functionally links those polymerases. The ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment was successfully crystallized. The crystals belong to the space group of P3_121 with the cell-dimension of a=b=74.7, c=124.5 Å and・=120°. Electron density map obtained from molecular replacement method using REV7-REV3 complex as a search model clearly shows that the REV1 C-terminal domain adopts a four-helices bundle structure.

Research Products

• [Presentation] Structure of REV7 in complex with a REV3 fragment and structural implication of the interaction between DNA polymeraseζ and REV1 (2011)
  • Author(s): Hiroshi Hashimoto, Kodai Hara, Yoshiki Murakumo, Shunsuke Kobayashi, Toshiaki Kogame, Satoru Unzai, Satoko Akashi, Shunichi Takeda, Toshiyuki Shimizu, Mamoru Sato
  • Organizer: NH Grand Hotel Krasnapolsky
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 20110607-09
• [Presentation] Structure of REV7 in complex with a REV3 fragment and structural implication of the interaction between DNA polymeraseζand REV1 (2011)
  • Author(s): Hiroshi Hashimoto
  • Organizer: SNY-YCU Bilateral Symposium on Structural Biology
  • Place of Presentation: RIEKN Yokohama Institute, Yokohama, Japan
  • Year and Date: 2011-11-14
• [Presentation] Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymeraseζ and REV1 (2010)
  • Author(s): Hiroshi Hashimoto, Kodai Hara, Yoshiki Murakumo, Shunsuke Kobayashi, Toshiaki Kogame, Satoru Unzai, Satoko Akashi, Shunichi Takeda, Toshiyuki Shimizu, Mamoru Sato
  • Organizer: 3R meeting
  • Place of Presentation: Toyama Conference Center
  • Year and Date: 20101016-30
• [Presentation] 損傷乗り越えDNA合成におけるタンパク質間相互作用の構造生物学 (2010)
  • Author(s): 橋本博
  • Organizer: 第18回大阪府立母子保健総合医療センターシンポジウム(母と子のすこやか基金)
  • Place of Presentation: 大阪府立母子保健総合医療センター研究所
  • Year and Date: 2010-12-24