Structural basis of a novel Wnt signaling regulation by dynamic oligomerized proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770112
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Gunma University (2011); University of Hyogo (2010)
• Principal Investigator: TERAWAKI Shin-ichi 群馬大学, 大学院・工学研究科, 助教 (10452533)
• Project Period (FY): 2010 – 2011
• Keywords: 動的オリゴマー形成 / Wntシグナル伝達 / DIXドメイン

Research Abstract

The Wnt signaling pathway plays an essential role in cell growth, differentiation, polarity formation and neural development. Coiled-Coil DIX1 (CCD1) and Axin have a novel ability to form dynamic oligomers and the complex formation. To elucidate the interaction between CCD1 and Axin, we have been carried out structural studies of the CCD1-Axin complex using X-ray crystallography. We established the preparation of the DIX domains of both proteins, which are necessary for the dynamic oligomerization and the complex formation. We performed crystallization trials on the CCD1-Axin complex. The crystals were unsuitable for crystallographic analysis because their diffraction quality was poor (around 20 Å).Improvement of the crystallization condition is required for the structural analysis of the CCD1-Axin complex.

Research Products

• [Journal Article] Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd1. (2011)
  • Author(s): Terawaki, S., Yano, K., Katsutani, T., Shiomi, K., Keino-Masu, K., Masu, M., Shomura, Y., Komori, H., Shibata, N. Higuchi Y.
  • Journal Title: Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. Volume: 67 Pages: 758-761
  • Peer Reviewed
• [Presentation] 微小管依存的な物質輸送を制御するBICDの構造生物学的研究 (2011)
  • Author(s): 寺脇慎一
  • Organizer: 超異分野学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-19
• [Presentation] Wntシグナル伝達で機能するCCD1 DIXドメインのX線結晶構造解析 (2010)
  • Author(s): 寺脇慎一, 塩見健輔, 桝正幸, 柴田直樹, 樋口芳樹
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Wntシグナル伝達で機能するCCD1のオリゴマー形成機構に関する構造学的研究 (2010)
  • Author(s): 寺脇慎一, 塩見健輔, 桝正幸, 柴田直樹, 樋口芳樹
  • Organizer: 日本結晶学会60周年記念年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-03
• [Presentation] Wntシグナル制御因子Dishevelled-DIXドメインのX線構造解析 (2010)
  • Author(s): 柴田直樹, 寺脇慎一, 小森博文, 庄村康人, Fiedler Marc, Bienz Mariann, 樋口芳樹
  • Organizer: 第10回日本蛋白質学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-17
• [Presentation] Wntシグナル伝達ではたらくCCD1の構造化学的研究 (2010)
  • Author(s): 寺脇慎一, 塩見健輔, 桝正幸, 柴田直樹, 樋口芳樹
  • Organizer: 第10回日本蛋白質学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-17