2012 Fiscal Year Final Research Report
Protein crystallization on zeolites as substrate materials
| Project/Area Number |
22770116
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUGAHARA Michihiro 独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 研究員 (00415192)
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| Project Period (FY) |
2010 – 2012
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| Keywords | X線結晶構造解析 / タンパク質結晶化 / ゼオライト / モレキュラーシーブ / ヘテロエピタキシャル |
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| Research Abstract |
Protein crystallization is still a major bottleneck in structural biology. In order to overcome this difficulty, an advanced crystallization method using synthetic zeolite molecular sieves (MS) as hetero-epitaxic nucleants by which a directed crystal nucleation on the material surface occurred in a wide variety of proteins, has been developed in this study. A sparsematrix screening experiment in the presence of MS or MS-like compounds has been performed, showing a clear improvement in the success rate of protein crystallization as compared with the conventional screening. The results suggested that micro-porous, crystalline and metal-ion-binding materials promote nucleation of proteins.
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[Journal Article] Autolabo: an automated system for ligand-soaking experiments with protein crystals2010
Author(s)
Michihiro Sugahara, Katsumi Shimizu, Yukuhiko Asada, Hideki Fukunishi, Hirohumi Kodera, Takeshi Fujii, Eiji Osada, Takashi Kasazaki, Toshihumi Sawada, Hideyuki Chikusa, Kazuaki Kondo, Akira Yorihiro, Naoki Kunishima
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Journal Title
J. Appl. Crystallogr.
Volume: 43
Pages: 940-944
DOI
Peer Reviewed
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