2011 Fiscal Year Final Research Report
Reaction Mechanism of Heme Detoxification Enzyme
| Project/Area Number |
22770141
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
ISHIKAWA Haruto 大阪大学, 大学院・理学研究科, 講師 (40551338)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 酵素の触媒機構 / ヘム |
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| Research Abstract |
Free heme is a pro-oxidant and catalyzes the production of reactive oxygen species that is toxic to cells. Heme detoxification protein(HDP), potent in converting heme into an insoluble crystalline form called hemozoin(Hz), was found in the malaria parasite. We succeeded in the high-yield expression of recombinant HDP in E. coli. ESR and resonance Raman measurements for the HDP-heme complex revealed that a histidine residue binds heme.
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