An electron microscopic study of actin nucleation factors

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: NARITA Akihiro 名古屋大学, 理学研究科, 助教 (30360613)
• Project Period (FY): 2010 – 2011
• Keywords: アクチン / 電子顕微鏡 / 電子線トモグラフィー / 細胞骨格 / 画像解析 / Arp2/3複合体 / フォルミン

Research Abstract

The actin filament is responsible for a wide range of movement of the cell and dynamic through construction and destruction. Many proteins regulate the dynamics of the actin filament. Arp2/3 complex and formin are ones of the regulatory proteins and they start the construction of the actin filament in the cell. To understand mechanism of starting construction, we developed structural analysis procedures of the actin filament network in the cell with an Austrian group, which determined distribution and structure of Arp2/3 complex in the cell by using electron microscopy and image analysis. We also elucidated that formin binds only to the ends of the actin filament, not the side. Now we are analyzing data to conform the bound end to formin is the barbed end.

Research Products

• [Journal Article] A novel actin-like filament structure from Clostridium tetani (2012)
  • Author(s): Popp, D., Narita, A., Lee, LJ., Ghoshdastider, U., Xue, B., Srinivasan, R., Balasubramanian, MK., Tanaka, T., Robinson, RC.
  • Journal Title: J. Biol. Chem. Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Direct determination of actin polarity in the cell (2012)
  • Author(s): Narita, A., Mueller, Jan., Vinzenz, M., Small, JV., Maeda, Y.
  • Journal Title: Mol. Biol.
  • DOI: doi:10.1016/j.jmb
  • Peer Reviewed
• [Journal Article] Actin branching in the initiation and maintenance of lamellipodia (2012)
  • Author(s): Vinzenz, M., Nemathova, M., Schur, F., Mueller, J., Narita A., Urban, E., Winkler, C., Schmeiser, C., Koestler, SA., Rottner, K., Resch, GP., Meada, Y., Small, JV
  • Journal Title: J. Cell. Sci.
  • DOI: doi:10.1242/jcs.107623
  • Peer Reviewed
• [Journal Article] Merits of the double-stranded form of the actin filament revealed by structures of the filament ends (2011)
  • Author(s): Narita A.
  • Journal Title: Communicative and Integrative biology 4 Volume: 4 Pages: 692-695
  • Peer Reviewed
• [Journal Article] Minimum requirements for the actin-like treadmilling motor system (2011)
  • Author(s): Narita A
  • Journal Title: BioArchitecture Volume: 1 Pages: 205-208
  • Peer Reviewed
• [Presentation] 繊維状タンパク質複合体のための単粒子解析法 (2012)
  • Author(s): 成田哲博
  • Organizer: 顕微鏡学会生体構造解析分科会
  • Place of Presentation: 湯沢ニューオータニホテル
  • Year and Date: 2012-12-27
• [Presentation] 分子と細胞をつなぐ線維状蛋白質構造解析 (2012)
  • Author(s): 成田哲博
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学(甲府市)
  • Year and Date: 2012-03-28
• [Presentation] Structural analysis of the actin filament in vitro and in vivo International Workshop" from Structure to Dynamics (2012)
  • Author(s): Narita A.
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-03-17
• [Presentation] Spatial structure of cytoskeleton associated with nuclear membrane International Workshop "from Structure to Dynamics" (2012)
  • Author(s): Minakata S., Koike M., Narita A., Maeda Y., Usukura J.
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-03-16
• [Presentation] Supramolecular structural analysis by STEM electron tomography International Workshop "from Structure to Dynamics" (2012)
  • Author(s): Koike M., Minakata S. Narita A., Homma M., Usukura J., Maeda Y.
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-03-16
• [Presentation] New method for identify the ABP binding sites on the actin filament International Workshop "from Structure to Dynamics" (2012)
  • Author(s): Dai S., Narita A., Ito T., Akaoka K., Maeda Y.
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-03-16
• [Presentation] Actin Polymerization correlates with the flattening of actin molecule 56th Annual meeting Biophysical Society (2012)
  • Author(s): Iwasa M., Maeda K., Narita A., Aihara T., Maeda Y., Oda T.
  • Place of Presentation: San Diego Convention Center(San Diego)
  • Year and Date: 2012-02-27
• [Presentation] 大阪大学蛋白質研究所セミナー (2012)
  • Author(s): 成田哲博
  • Organizer: 大阪大学蛋白質研究所セミナー
  • Place of Presentation: 大阪大学蛋白質研究所
  • Year and Date: 2012-02-17
• [Presentation] Structural analysis of the actin filament network in the cell Direction of future in biological electron microscopy (2012)
  • Author(s): Narita A.
  • Place of Presentation: 東京大学(文京区)
  • Year and Date: 2012-02-17
• [Presentation] Structural analysis of the actin filament in vivo and in vitro Joint Seminar Centre for BioImaging Sciences-Structural biology division of Japan EM (2012)
  • Author(s): Narita A.
  • Place of Presentation: シンガポール国立大学(Singapore)
  • Year and Date: 2012-01-12
• [Presentation] Structural analysis of Na+driven flagellar motor embedded in the membrane of Vibrio alginolyticus by electron tomography Sensory Transduction in Microorganisms at Grordon Research Conference (2011)
  • Author(s): Koike M., Narita A., Homma M., Maeda Y.
  • Year and Date: 20110100
• [Presentation] 分子から細胞までをつなぐ繊維状蛋白質複合体構造解析 (2011)
  • Author(s): 成田哲博
  • Organizer: 第2回神経科学と構造生物学の融合
  • Place of Presentation: 岡崎コンファレンスセンター(岡崎市)
  • Year and Date: 2011-11-22
• [Presentation] Two distinct mechanisms for actin capping protein regulation EMBO Lecture Courese The 26th (2011)
  • Author(s): Takeda S., Minakata S., Koike R., Kawahata I., Narita A., Kitazawa M., Ota M., Yamakuni T., Maeda Y., Nitanai Y.
  • Organizer: European Cytoskeletal Forum Meeting "Actin-Based Motility
  • Place of Presentation: Hotel Palma(Stresa,-Lake Maggiore)
  • Year and Date: 2011-10-30
• [Presentation] Narita A High-Resolution structural determination of the cofilin-decorated actin filament by single-particle analysis of cryo-electron micrographs EMBO Lecture Courese The 26th (2011)
  • Author(s): Tanaka K., Kimura-Sakiyama C., Dai Shuheng., Maeda Y.
  • Organizer: European Cytoskeletal Forum Meeting "Actin-Based Motility
  • Place of Presentation: Hotel Palma(Stresa,-Lake Maggiore)
  • Year and Date: 2011-10-30
• [Presentation] Visualization of Vibrio Na+-driven flagellar motor structure embedded in cell membrane (2011)
  • Author(s): Koike M., Narita A., Honma M., Maeda Y.
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2011-09-18
• [Presentation] High-resolution structural determination of the cofilin-decorated actin filament by single-particle analysis of cryo-electron micrographs (2011)
  • Author(s): Tanaka K., Kimura-Sakiyama C., Dai Shuheng., Maeda Y., Narita A.
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2011-09-18
• [Presentation] A structural analysis of actin cytoskeleton in the cell (2011)
  • Author(s): Narita A., Muller J., Urban E., Vinzenz M., Small J V., Maeda Y.
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2011-09-16
• [Presentation] 細胞骨格のための単粒子解析法"Single particle analysis for cytoskeletal proteins (2011)
  • Author(s): 成田哲博
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク(吹田市)
  • Year and Date: 2011-06-07
• [Presentation] Single particle analysis for actin filaments and other cytoskeletal filaments 2010年顕微鏡学会 (2010)
  • Author(s): 成田哲博
  • Organizer: アジア若手シンポジウム
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-05-23
• [Remarks]
  • URL: http://str.bio.nagoya-u.ac.jp:8080/Plone