2012 Fiscal Year Final Research Report
Characterization of the excited state of prion protein using high-pressure NMR technique
| Project/Area Number |
22770158
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Kinki University |
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Principal Investigator |
MAENO Akihiro 近畿大学, 先端技術総合研究所, 研究員 (70570951)
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| Project Period (FY) |
2010 – 2012
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| Keywords | プリオン蛋白質 / 核磁気共鳴 / 圧力 / 励起構造 |
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| Research Abstract |
The purpose of this study is to clarify the conformational basis of infection mechanism and to characterize the excited state of prion protein which causes a series of prion diseases defined by prion hypothesis. We established the protocols for the high-level expression and the purification of 15N-labeled full length hamster prion. In the experiment of NMR transverse relaxation dispersion that expected to enable the quantitative analysis of the excited state which is stabilized under pressure, the typical CPMG profiles have not been obtained and those experimental conditions have failed to be optimized. These results suggest that conformational fluctuations between the basic folded state and the excited state for haPrPC may take place beyond detection range (milli-second time scale) of NMR R2 dispersion measurement.
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Research Products
(9 results)
