2011 Fiscal Year Final Research Report
The crosstalk of DNA replication and sister chromatid cohesion in S phase
Project/Area Number |
22770168
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Molecular biology
|
Research Institution | The University of Tokyo |
Principal Investigator |
KAMATA Mkiko 東京大学, 分子細胞生物学研究所, 特任助教 (30572911)
|
Project Period (FY) |
2010 – 2011
|
Keywords | 染色体構築 / 機能 / 分配 |
Research Abstract |
We could gain the monoclonal antibody against acetylated cohesin for yeast and human respectively from a few hundreds of candidates. Yeast antibody did not work for ChIP(Chromatin immunoprecipitation) but human antibody could. We have developed the method to carry out ChIP-seq from reduced number of cells(around 10, 000 cells), and use this antibody to detect dynamics of Cohesin acetylation in human
|
Research Products
(3 results)