The crosstalk of DNA replication and sister chromatid cohesion in S phase
| Project/Area Number |
22770168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAMATA Mkiko 東京大学, 分子細胞生物学研究所, 特任助教 (30572911)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 染色体構築 / 機能 / 分配 / 遺伝子 / ゲノム / 細胞・組織 |
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| Research Abstract |
We could gain the monoclonal antibody against acetylated cohesin for yeast and human respectively from a few hundreds of candidates. Yeast antibody did not work for ChIP(Chromatin immunoprecipitation) but human antibody could. We have developed the method to carry out ChIP-seq from reduced number of cells(around 10, 000 cells), and use this antibody to detect dynamics of Cohesin acetylation in human
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Report
(3 results)
Research Products
(6 results)
