| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
We could gain the monoclonal antibody against acetylated cohesin for yeast and human respectively from a few hundreds of candidates. Yeast antibody did not work for ChIP(Chromatin immunoprecipitation) but human antibody could. We have developed the method to carry out ChIP-seq from reduced number of cells(around 10, 000 cells), and use this antibody to detect dynamics of Cohesin acetylation in human
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