Mechanisms and roles of cell binucleation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22770196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Gakushuin University
• Principal Investigator: TANIGUCHI Kiichiro 学習院大学, 理学部生命科学科, 助教 (20554174)
• Project Period (FY): 2010 – 2012
• Keywords: ショウジョウバエ / 二核細胞 / 生殖器附属腺 / 細胞分裂 / 細胞周期

Research Abstract

Some cells such as myocytes and hepatocytes can contain two nuclei. In this research project, I examined mechanisms and roles of the binucleation using the Drosophila tissue. In the result, we identified Mud which is a key factor to control the behavior of binucleating cells. We also found that binucleate state enables a cell to effectively change its shape by rearrangement of two nuclei for a plasticity of organ shape.

Research Products

• [Journal Article] The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. (2012)
  • Author(s): Taniguchi, K., Maeda, R., Ando, T., Okumura, T., Minami, R., Wakabayashi, M., Sugimori, S., Taniguchi, K., Kokuryo, A., Imano, T., Adachi-Yamada, T., Watanabe, N. and Nakagoshi, H.
  • Journal Title: PLoS One Volume: 7 Pages: 32302
  • Peer Reviewed
• [Journal Article] Reduced cell number in the hindgut epithelium disrupts hindgut left-right asymmetry in a mutant of pebble, encoding a RhoGEF, in Drosophila embryos (2012)
  • Author(s): Nakamura, M., Matsumoto, K., Iwamoto, Y., Muguruma, T., Nakazawa, N., Hatori, R., Taniguchi, K., Maeda, R. and Matsuno, K.
  • Journal Title: Mechanisms of Development Volume: 130 Pages: 169-180
  • DOI: DOI:10.1016/j.mod.2012.09.007
  • Peer Reviewed
• [Journal Article] Canonical Wnt signaling in the visceral muscle is required for left-right asymmetric development of the Drosophila midgut. (2011)
  • Author(s): Kuroda, J., Nakamura, M., Yoshida, M., Yamamoto, H., Maeda, T., Taniguchi, K., Nakazawa, N., Hatori, R., Ishio, A., Ozaki, A., Shimaoka, S., Ito, T., Iida, H., Okumura, T., Maeda, R. and Matsuno, K.
  • Journal Title: Mechanisms of Development Volume: 128 Pages: 625-639
  • DOI: DOI:10.1016/j.mod.2011.12.002
  • Peer Reviewed
• [Journal Article] Binucleation of Drosophila adult male accessory gland cells increases plasticity of organ size for effective reproduction. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Journal Title: Biological Systems- Open Access Volume: 1 Pages: 101
  • DOI: DOI:10.4172/BSO.1000e101
• [Journal Article] Chirality in planar cell shape contributes to left-right asymmetric epithelial morphogenesis. (2011)
  • Author(s): Taniguchi, K., Maeda, R., Ando, T., Okumura, T., Nakazawa, N., Hatori, R., Nakamura, M., Hozumi, S., Fujiwara, H. and Matsuno, K.
  • Journal Title: Science Volume: 333 Pages: 339-341
  • DOI: DOI:10.1126/science.1200940
  • Peer Reviewed
• [Journal Article] Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila (2010)
  • Author(s): Okumura, T., Fujiwara, H., Taniguchi, K., Kuroda, J., Nakazawa, N., Nakamura, M., Hatori, R., Ishio, A., Maeda, R. and Matsuno, K.
  • Journal Title: Developmental Biology Volume: 344 Pages: 693-706
  • DOI: DOI:10.1016/j.ydbio.2010.05.501
  • Peer Reviewed
• [Journal Article] A novel Cre/loxP system for mosaic gene expression in the Drosophila embryo.
  • Author(s): Nakazawa, N., Taniguchi, K., Okumura, T., Maeda, R. and Matsuno, K.
  • Journal Title: Developmental Dynamics Volume: 241 Pages: 965-974
  • DOI: DOI:10.1002/dvdy.23784
  • Peer Reviewed
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2012)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 53rd Annual Drosophila Research Conference
  • Place of Presentation: Sheraton Chicago Hotel & Towers (米国)
  • Year and Date: 20120308-10
• [Presentation] Elimination of malfunctioning cells in Drosophila male accessory gland, a non-regenerative tissue. (2012)
  • Author(s): Taniguchi, K., Abe, S., Minami, R., Imano, T., Kokuryo, A., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 劍潭青年活動中心 (台湾)
  • Year and Date: 20110522-25
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing a higher level of plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-18
• [Presentation] Cytokinesis- deficient binucleation as a strategy for tissue enlargement in Drosophila. (2010)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Book] 実験医学 (2012)
  • Author(s): 中澤直高、谷口喜一郎、前田礼男、安藤格士、松野健治
  • Total Pages: 75-78
  • Publisher: 羊土社
• [Book] 細胞工学 (2011)
  • Author(s): 前田礼男、谷口喜一郎、安藤格士、松野健治
  • Total Pages: 1298-1300
  • Publisher: 学研メディカル秀潤社
• [Remarks]
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/