2011 Fiscal Year Final Research Report
Analysis of dynamics of histone modifications during embryogenesis of the ascidian Ciona intestinalis
| Project/Area Number |
22770223
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
WADA Shuichi 長浜バイオ大学, バイオサイエンス学部, 講師 (20378607)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 遺伝子発現調節 / ヒストン修飾 |
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| Research Abstract |
To understand the role of histone modifications in animal embryogenesis, the histone methylation pattern and the expression of genes for histone lysine methyltransferases(HKMTs) and histone lysine demethylases(HKDMs) during ascidian embryogenesis were investigated. It has been shown that histone H3 lysine 4(H3K4) methylation is an active mark for transcription while histone H3 lysine 27(H3K27) methylation marks repressive states of transcription. ChIP-qPCR analysis of the distribution of methylated H3K4 in several selected regions of the genome of Ciona tailbud stage embryos revealed that the methylated H3K4 mark is associated with the transcribed region of active genes. Expression patterns of almost all HKMT and HKDM genes were examined and genes that are expressed during Ciona embryogenesis were identified.
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