Live-imaging analysis of mitochondrial movement in rice cells during rice. Magnaporthe oryzae interactions

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: MOCHIZUKI Susumu 独立行政法人農業生物資源研究所, 遺伝子組換え研究センター耐病性作物研究開発ユニット, 特別研究員 (40567020)
• Research Collaborator: NISHIZAWA Yoko 農業生物資源研究所, 耐病性作物研究開発ユニット, 上級研究員 (40355756) ; KUBO Yasuyuki 京都府立大学, 生命環境科学研究科, 教授 (80183797) ; YAMANE Hisakazu 帝京大学, 理工学部・バイオサイエンス学科, 教授 (80090520) ; OKADA Kazunori 東京大学, 生物生産工学研究センター, 助教 (20312241)
• Project Period (FY): 2010 – 2011
• Keywords: ミトコンドリア / イネ / いもち病菌 / 病害抵抗性 / ライブイメージング

Research Abstract

I improved the live and real-time imaging method using trimmed and sliced leaf-sheath to analyze the structural changes and the intracellular reactions both in a single cell of rice and blast fungus during the early infection process. Using this method, two types of alteration of mitochondrial distribution were observed. Observation using some mutants of the rice blast fungus revealed that both of types of the alteration were closely related to resistant reaction of rice cells to the blast. Moreover, I applied the imaging method to observe some cellular reactions during the infection and wounding.

Research Products

• [Journal Article] Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice (2012)
  • Author(s): Koji Miyamoto, Takafumi Shimizu, Susumu Mochizuki, Yoko Nishizawa, Eiichi Minami, Hideaki Nojiri, Hisakazu Yamane and Kazunori Okada
  • Journal Title: Protoplasma Volume: (in press)
  • DOI: DOI:10.1007/s00709-012-0400-z
  • Peer Reviewed
• [Journal Article] Expression of a bacterial chitosanase in rice plants improve disease resistance to the rice blast fungus Magnaporthe oryzae (2012)
  • Author(s): Yusuke Kouzai, Susumu Mochizuki, Akihiro Saito, Akikazu Ando, Eiichi Minami and Yoko Nishizawa
  • Journal Title: Plant Cell Reports Volume: 31 Pages: 629-636
  • DOI: DOI:10.1007/s00299-011-1179-7
  • Peer Reviewed
• [Journal Article] Localization of probe-accessible chitin and characterization of genes encoding chitin-binding domains during rice-Magnaporthe oryzae interactions (2011)
  • Author(s): Susumu Mochizuki, Ken-ichiro Saitoh, Eiichi Minami and Yoko Nishizawa
  • Journal Title: Journal of general plant pathology Volume: 77 Pages: 163-173
  • DOI: DOI:10.1007/s10327-011-0310-5
  • Peer Reviewed
• [Presentation] イネいもち病菌感染初期過程におけるミトコンドリア凝集反応の観察 (2012)
  • Author(s): 望月進,齋藤憲一郎,久保康之,南栄一,西澤洋子
  • Organizer: 日本植物病理学会
  • Place of Presentation: 福岡
  • Year and Date: 20120300
• [Presentation] 細菌キトサナーゼを発現するイネの作製とそのいもち病抵抗性の解析 (2012)
  • Author(s): 香西雄介,望月進,齋藤明広,安藤昭一,南栄一,西澤洋子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20120300
• [Presentation] 高濃度窒素条件下におけるイネユビキチンリガーゼEL5の機能解析 (2012)
  • Author(s): 望月進,中島恵美,軸丸祐介,神谷勇治,南栄一,西澤洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 20120300
• [Presentation] キトサン分解能を付与した形質転換イネの作出とそのいもち病抵抗性の解析 (2011)
  • Author(s): 香西雄介,望月進,齋藤明広,安藤昭一,南栄一,西澤洋子
  • Organizer: 日本植物病理学会関東部会
  • Place of Presentation: つくば
  • Year and Date: 20110900
• [Presentation] Biological Function of the JA-inductive bHLH Transcription Factor RERJ1 in Rice Terpenoids Synthesis (2011)
  • Author(s): Kazunori Okada, Koji Miyamoto, Takafumi Shimizu, Tatsuya Kitajima, Koichi Kawamoto, Tetsuya Chujo, Rika Ozawa, Junji Takabayashi, Susumu Mochizuki, Yoko Nishizawa, Akio Miyao, Hirohiko Hirochika, Hideaki Nojiri, Hisakazu Yamane
  • Organizer: 10th International Meeting : Biosynthesis and Functional Isoprenoids in Plants
  • Place of Presentation: Microorganisms and Parasites
  • Year and Date: 20110500
• [Presentation] イネ剥離葉鞘裏面細胞を用いたいもち病菌感染初期における細胞内構造変化のリアルタイムイメージング法の構築 (2011)
  • Author(s): 望月進,南栄一,西澤洋子
  • Organizer: 日本植物病理学会
  • Place of Presentation: 東京
  • Year and Date: 20110300
• [Presentation] いもち病菌感染初期におけるイネの細胞内構造変化の可視化 (2010)
  • Author(s): 望月進,南栄一,西澤洋子
  • Organizer: 日本植物病理学会
  • Place of Presentation: 京都
  • Year and Date: 20100400
• [Book] Defense mechanisms mediated by chitin in rice-blast interactions. Genome-Enabled Analysis of Plant-Pathogen Interactions (2011)
  • Author(s): Yoko Nishizawa, Susumu Mochizuki, Ken-ichiro Saitoh, Kyutaro Kishimoto, Yusuke Kouzai, Eiichi Minami
  • Total Pages: 121-129
  • Publisher: The American Phytopathological Society Press