2012 Fiscal Year Final Research Report
Development and application of a method for efficient gene transfer of non-viral DNA targeted to murine liver
| Project/Area Number |
22780274
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | National Defense Medical College |
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Principal Investigator |
NAKAMURA Shingo 防衛医科大学校, 医学教育部医学科専門課程, 助教 (00505323)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遺伝子導入 / 遺伝子発現制御 / 疾患モデル / 組織特異性 / 非ウイルスベクター |
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| Research Abstract |
In this study, we attempted to improve hydrodynamics-based gene delivery (HGD) to achieve a highly efficient gene delivery that is targeted to the murine liver and tested whether the liver-specific expression of a gene of interest is possible. We demonstrated that upon performing HGD, mixing of plasmid DNA with polyethylenimine (PEI)-based reagent yielded best transfection efficiency in hepatocytes. With this improved technique, we succeeded in performing Cre-loxP-mediated gene switching in liver and to show lines of direct evidence for liver specific transgene expression.
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