2012 Fiscal Year Final Research Report
Development of rice transporter gene enrichment library to isolatenovel arsenic transporter genes.
| Project/Area Number |
22780291
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Boundary agriculture
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | トランスポーター / ヒ素 / 重金属の転流機構 |
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| Research Abstract |
The yeast expression library enriched with rice transporter genes was developed here. About 1,500 full length cDNAs were obtained from resource center and sub-cloned into yeast expression vector (pYES2). These plasmid were transformed into Saccharomyces cerevisiae and glycerol stocked. The library was screened on SD medium containing arsenic and arsenate. Total 17 As sensitive clones were screened.
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