2011 Fiscal Year Final Research Report
Establishment of a laticifer-specific gene expression system in Hevea brasiliensis
| Project/Area Number |
22780298
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
TAKAHASHI Seiji 東北大学, 大学院・工学研究科, 准教授 (90343061)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 発現制御 / 天然ゴム |
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| Research Abstract |
Natural rubber obtained from latex in Hevea brasiliensis has the excellent physical properties, especially required for tire manufacturing. To meet the continuously increasing demand for natural rubber, metabolic engineering of H. brasiliensis is required to improve natural rubber production, achieved by a latex-specific overexpression of genes involved in the natural rubber biosynthesis. In this study, we have succeeded in discovering of latex-specific genes and cloning of 5'-upstream regions of these latex specific genes. Furthermore, we also identified several latex-specific transcription factors, expected to participate in the regulation of the latex-specific gene expression.
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Research Products
(16 results)
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[Journal Article] Transcription analyses of GmICHG, a gene coding for a .-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr2013
Author(s)
Yoo, D., Hara, T., Fujita, N., Waki, T., Noguchi, A., Takahashi, S. and Nakayama, T.
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Journal Title
Plant Science
Volume: 208
Pages: 10-19
DOI
Peer Reviewed
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[Journal Article] Purification, Gene Cloning, and Biochemical Characterization of a beta-Glucosidase Capable of Hydrolyzing Sesaminol Triglucoside from Paenibacillus sp. KB05492013
Author(s)
Nair, A., Kuwahara, A., Nagase, A., Yamaguchi, H., Yamazaki, T., Hosoya, M., Omura, A., Kiyomoto, K., Yamaguchi, M.A., Shimoyama, T., Takahashi, S., and Nakayama, T
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Journal Title
PLoS One
Volume: 8
Pages: e60538
DOI
Peer Reviewed
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[Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1)2010
Author(s)
Hatayama, M., Unno, H., Kusunoki, M., Takahashi, S., Nishino, T., and Nakayama, T
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Journal Title
Journal of Bioscience and Bioengineering
Volume: 110
Pages: 158-164
DOI
Peer Reviewed
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