2012 Fiscal Year Final Research Report
Discovery of a biosynthetic gene cluster for an anti-tubercular agent its application for the establishment of overproduction system for the anti-tubercular agent and the production of anti-cancer drug-
| Project/Area Number |
22790068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUMAGAI Takanori 広島大学, 大学院・医歯薬保健学研究院, 准教授 (70274058)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 抗結核薬 / D-サイクロセリン / 生合成 / 異種発現 / 放線菌 |
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| Research Abstract |
In the present study, the biosynthetic pathway for the anti -tubercular agent, D-cycloserine (DCS), was elucidated by the gene disruption analysis of the DCS biosynthetic gene cluster in a DCS-producing Streptomyces lavendulae ATCC11924. In addition, DCS production system in Escherichia (E.) coli was established by introducing the DCS biosynthetic genes into E. coli. Furthermore, DcsA was found to be a novel heme protein involved in the biosynthesis of DCS.
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[Journal Article] Establishment of an in vitroD-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway2013
Author(s)
Uda, N., Matoba, Y., Kumagai, T., Oda, K., Noda, M., and Sugiyama, M
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Journal Title
Antimicrob. Agents Chemother
Pages: in press
DOI
Peer Reviewed
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[Journal Article] Heme protein and hydroxyarginase necessary for biosynthesis of D-cycloserine2012
Author(s)
Kumagai, T., Takagi, K., Koyama, Y., Matoba, Y., Oda, K., Noda, M., and Sugiyama, M
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Journal Title
Antimicrob. Agents Chemother
Volume: 56
Pages: 3682-3689
DOI
Peer Reviewed
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[Journal Article] Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae. Antimicrob2010
Author(s)
Kumagai, T., Koyama, Y., Oda, K., Noda, M., Matoba, Y., and Sugiyama, M
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Journal Title
Agents Chemother
Volume: 54
Pages: 1132-1139
DOI
Peer Reviewed
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[Presentation] A hydroxylation reaction necessary in the biosynthesis of an antitubercular antibiotic D-cycloserine2011
Author(s)
Kumagai, T., Takagi, K., Noda, M., Oda, K., Matoba, Y., and Sugiyama, M
Organizer
IUMS2011
Place of Presentation
Sapporo, Japan
Year and Date
20110906-10
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