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2011 Fiscal Year Final Research Report

Establishment of in vitro selection system using recent primary isolates, and a new efficient treatment for HIV

Research Project

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Project/Area Number 22790163
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Medical pharmacy
Research InstitutionKumamoto University

Principal Investigator

HARADA Shigeyoshi  熊本大学, エイズ学研究センター, COEリサーチ・アソシエイト (30508643)

Project Period (FY) 2010 – 2011
Keywordsオーダーメード医療 / HIV
Research Abstract

In vitro selection with anti-HIV-1 drugs were used to obtain genetically heterogeneous primary HIV-1 isolates. Env diversities significantly decreased during in vitro selection. Furthermore, these drugs-selected variants had completely different Env sequences from that in the passage control. This study is the first to explore the influence of anti-HIV drugs on genetic bottlenecks in bulk HIV primary isolates containing highly diverse Env sequences using in vitro selection methods. In vitro selection may be a useful tool for investigating the compatibility of combined anti-retroviral therapy using entry inhibitors.

  • Research Products

    (12 results)

All 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] In vitro and In vivo Resistance to Human Immunodeficiency Virus Type 1 Entry Inhibitors2011

    • Author(s)
      Maeda, Y., Yoshimura, K., Miyamoto, F., Kodama, E., Harada, S., Yuan, Y., Harada, S., and Yusa, K
    • Journal Title

      J AIDS Clinic Res

    • Peer Reviewed
  • [Journal Article] Small molecular CD4 mimics as HIV entry inhibitors2011

    • Author(s)
      Narumi, T., Arai, H., Yoshimura, K., Harada, S., Nomura, W., Matsushita, S., Tamamura. H
    • Journal Title

      Bioorg Med Chem

      Volume: 19 Pages: 6735-6742

    • Peer Reviewed
  • [Journal Article] CD4 mimics targeting the HIV entry mechanism and their hybrid molecules with a CXCR4 antagonist2010

    • Author(s)
      Narumi, T., Ochiai, C., Yoshimura, K., Harada, S., Tanaka, T., Nomura, W., Arai, H., Ozaki, T., Ohashi, N., Matsushita, S., Tamamura, H
    • Journal Title

      Bioorg Med Chem Lett

      Pages: 5853-5858

    • Peer Reviewed
  • [Journal Article] Enhanced exposure of human immunodeficiency virus type 1 primary isolate neutralization epitopes through binding of CD4 mimetic compounds2010

    • Author(s)
      Yoshimura, K., Harada, S., Shibata, J., Hatada, M., Yamada, Y., Ochiai, C., Tamamura, H., Matsushita S
    • Journal Title

      J. Virol

      Volume: 84 Pages: 7558-7568

    • Peer Reviewed
  • [Journal Article] HIV-1 evasion of a neutralizing anti-V3 antibody involves acquisition of a potential glycosylation site in V22010

    • Author(s)
      Hatada, M., Yoshimura, K., Harada, S., Kawanami, Y., Shibata, J., Matsushita S
    • Journal Title

      J. Gen. Virol

      Volume: 91 Pages: 1335-1345

    • Peer Reviewed
  • [Journal Article] CD4 mimics targeting the mechanism of HIV entry2010

    • Author(s)
      Yamada, Y., Ochiai, C., Yoshimura, K., Tanaka, T., Ohashi, N., Narumi, T., Nomura, W., Harada, S., Matsushita, S., Tamamura. H
    • Journal Title

      Bioorg Med Chem Lett

      Volume: 20 Pages: 354-358

    • Peer Reviewed
  • [Presentation] 抗HIV剤がEnv多様性に与える影響2011

    • Author(s)
      原田恵嘉、濱治有希、遊佐敬介、松下修三、吉村和久
    • Organizer
      第25回日本エイズ学会学術集会・総会
    • Place of Presentation
      ハイアットリージェンシー東京(東京)
    • Year and Date
      2011-12-01
  • [Presentation] Impact of raltegravir pressure on the selection of HIV-1 envelope sequences in vitro2011

    • Author(s)
      Harada S., Ishikawa T., Hamji A., Matsushita S., Yoshimura K
    • Organizer
      12th Kumamoto AIDS Seminar GCOE Joint International Symposium
    • Place of Presentation
      Aso Resort GRANDVRIO Hotel(Kumamoto, Japan)
    • Year and Date
      2011-10-21
  • [Presentation] Evolution and selection of the env gene of HIV-1 primary isolates during in vitro selection of raltegravir2011

    • Author(s)
      Harada S., Hamji A., Matsushita S., Yoshimura K
    • Organizer
      11th Kumamoto AIDS Seminar GCOE Joint International Symposium
    • Place of Presentation
      Aso Resort GRANDVRIO Hotel(Kumamoto, Japan)
    • Year and Date
      2011-10-08
  • [Presentation] Impact of raltegravir pressure on the selection of HIV-1 envelope sequences in vitro2011

    • Author(s)
      Harada S., Ishikawa T., Hamji A., Matsushita S., Yoshimura K
    • Organizer
      6th IAS Conference on HIV Pathogenesis, Treatment and Prevention
    • Place of Presentation
      Auditorium Parco della Musica(Roma, Italy)
    • Year and Date
      2011-07-17
  • [Presentation] ラルテグラビルはHIV-1のin vitro馴化におけるEnv選択に影響する2010

    • Author(s)
      原田恵嘉、濱治有希、松下修三、吉村和久
    • Organizer
      第24回日本エイズ学会学術集会・総会
    • Place of Presentation
      ザ・プリンスさくらタワー東京(東京)
    • Year and Date
      2010-11-25
  • [Remarks]

    • URL

      http://yoshimura-project.p1.bindsite.jp/index.html

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Published: 2013-07-31  

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