2011 Fiscal Year Final Research Report
Detection of state-dependent change of the stoichiometry of KCNQ1-KCNE1 complex by single molecule imaging
| Project/Area Number |
22790223
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
NAKAJO Koichi 生理学研究所, 分子生理研究系, 助教 (80390699)
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| Project Period (FY) |
2010 – 2011
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| Keywords | イオンチャネル / カリウムチャネル / 不整脈 / 複合体 / 一分子イメージング / 全反射蛍光顕微鏡 / 修飾サブユニット / ストイキオメトリー |
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| Research Abstract |
KCNQ1, which is known as a causative gene of cardiac arrhythmia, is an alpha subunit of voltage-gated K^+channel. KCNQ1 and its auxiliary subunit KCNE1 form an ion channel complex, which regulates the heartbeat. To determine the stoichiometry of this complex, I attached fluorescent tags on these subunits, and counted the number of each subunit in a single KCNQ1-KCNE1 complex by using total internal reflection microscope. One complex contained 1-4 KCNE1 subunits, and the number of KCNE1 was dependent on the relative expression densities of KCNQ1 and KCNE1 subunits.
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Research Products
(15 results)
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[Remarks] (3)のPNAS誌論文関連の報道日経産業新聞2010年10月20日掲載科学新聞2010年11月5日掲載
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[Remarks] PNAS誌による紹介記事"COMMENTARY"Osteen JD, Sampson, KJ, Kass, RS. The cardiac IKs channel, complex indeed. Proc. Natl. Acad. Sci. U. S. A. 107(2010) 18751-18752
