Molecular mechanisms controlling a front-rear axis in motile cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: WATANABE Takashi 名古屋大学, 大学院・医学系研究科, 特任講師 (10402562)
• Project Period (FY): 2010 – 2011
• Keywords: 細胞極性 / 細胞遊走 / 細胞内シグナル伝達 / Rhoファミリー / 細胞骨格

Research Abstract

Major aims in this research are to reveal the molecular mechanisms governing a front-rear polarity in motile cells. Migrating cells acquire front-rear polarity along the directional axis, with signaling molecules, adhesions and the cytoskeleton distributed asymmetrically. At the front of migrating cells, they maintain the higher activity of small GTPase Rac and persistently form adhesion sites toward substratum. Activated Rac at the front then controls microtubule dynamics. This research demonstrates that Tiam1 accumulates at adhesion sites and is responsible for Rac activation at the front of motile. Furthermore, this research also shows the molecular mechanisms controlling microtubule dynamics downstream of Rac in a region-specific manner.

Research Products

• [Journal Article] Role of a tyrosine phosphorylation of SMG-9 in binding of SMG-9 to IQGAP and the NMD complex (2011)
  • Author(s): Takeda S, Fujimoto A, Yamauchi E, Hiyoshi M, Kido H, Watanabe T, Kaibuchi K, Ohta T, Konishi H
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 29-33
  • Peer Reviewed
• [Journal Article] Numb controls E-cadherin endocytosis throughp120 catenin with a PKC (2011)
  • Author(s): Sato K, Watanabe T, Wang S, Kakeno M, Matsuzawa K, Matsui T, Yokoi K, Murase K, Sugiyama I, Ozawa M, Kaibuchi K
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 3103-3119
  • Peer Reviewed
• [Journal Article] EB1promotes microtubule dynamics by recruiting Sent in in Drosophila cells (2011)
  • Author(s): Li W, Miki T, Watanabe T, Kakeno M, Sugiyama I, Kaibuchi K, Goshima G
  • Journal Title: J Cell Biol Volume: 193 Pages: 973-983
  • Peer Reviewed
• [Journal Article] AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation (2010)
  • Author(s): Nakano A, Kato H, Watanabe T, Min KD, Yamazaki S, Asano Y, Seguchi O, Higo S, Shintani Y, Asanuma H, Asakura M, Minamino T., Kaibuchi K, Mochizuki N, Kitakaze M, Takashima S
  • Journal Title: Nat Cell Biol Volume: 12 Pages: 583-590
  • Peer Reviewed
• [Presentation] Tiam1 acts with the PAR complex to control talin-mediated Rac1 activation during polarized cell migration (2011)
  • Author(s): Watanabe T., et al.
  • Organizer: Gordon Research Conference, Gradient Sensing & Directional Cell Migration
  • Place of Presentation: Switzerland, Les Diablerets, Les Diablerets Conference Center
  • Year and Date: 20110608-09
• [Presentation] Phosphorylation of+TIPs during cell migration (2011)
  • Author(s): Watanabe T., et al.
  • Organizer: 2011 American Society for Cell Biology annual meeting
  • Place of Presentation: US, CO, Denver, Convention Center
  • Year and Date: 2011-12-03
• [Presentation] Tiam1 acts with the PAR complex to control talin-mediated Rac1 activation during polarized cell migration (2011)
  • Author(s): Watanabe T., et al.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道,札幌市,北海道大学
  • Year and Date: 2011-06-29
• [Presentation] Numb controls E-cadherin endocytosis acting through p120catenin with aPKC (2010)
  • Author(s): Watanabe T., et al.
  • Organizer: 50th annual meeting for American Society for Cell Biology
  • Place of Presentation: US, PA, Philadelphia, Convention Center
  • Year and Date: 2010-12-12
• [Presentation] Numb controls E-cadherin endocytosis acting through p120catenin with aPKC (2010)
  • Author(s): Watanabe T., et al.
  • Organizer: Gordon Research Conference, Signals By Adhesion Receptors
  • Place of Presentation: US, ME, Waterville, Colby College
  • Year and Date: 2010-07-15
• [Presentation] In vitro reconstitution of microtubule dynamics at cell cortex in migrating cells (2010)
  • Author(s): Watanabe T., et al.
  • Organizer: EMBO conference series. Microtubule-structure, regulation, and functions
  • Place of Presentation: Germany, Heidelberg, EMBL
  • Year and Date: 2010-06-03