2011 Fiscal Year Final Research Report

Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae

Research Project

Project/Area Number	22790537
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Laboratory medicine
Research Institution	財団法人神奈川科学技術アカデミー (2011) Kanagawa Academy of Science and Technology (2010)
Principal Investigator	NAKANO Ryuichi 財団法人神奈川科学技術アカデミー, 重点研究室光触媒グループ, 常勤研究員 (80433712)
Research Collaborator	ABE Michiko 北里大学, 医療衛生学部・臨床化学, 講師 OKAMOTO Ryoichi 北里大学, 医学部・微生物学, 講師
Project Period (FY)	2010 – 2011
Keywords	薬剤耐性菌 / QRDR / PCR-RFLP法 / グラム陰性菌
Research Abstract	Fluoroquinolone(FQ) resistance among Enterobacteriaceae species has been reported increasingly. It occurs primarily through mutation in the Quinolone Resistance-Determining Region(QRDR) of the DNA gyrase(gyrA) and topoisomerase IV(parC). I designed the mismatch PCR-RFLP to detect common mutations in QRDR of gyrA and parC. Developed mismatch PCR-RFLP successfully detected the mutations of gyrA and parC genes of Enterobacteriaceae clinical isolates with reduced susceptibility to FQs. This method offers an inexpensive, rapid, and easier alternative for detection of point mutations in FQ resistance in Enterobacteriaceae.

(3 results)