2011 Fiscal Year Final Research Report
Detection of non-culturable Legionella species by amoebal co-culture from bath water samples.
| Project/Area Number |
22790590
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
EDAGAWA Akiko 大阪府立公衆衛生研究所, 衛生化学部, 研究員 (80321941)
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| Research Collaborator |
KIMURA Akio 大阪府立公衆衛生研究所, 企画総務部, 課長 (00250283)
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| Project Period (FY) |
2010 – 2011
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| Keywords | レジオネラ / アカントアメーバ / アメーバ共培養法 / co-culture / 浴槽水 / 培養不能菌 / VNC(viable but non-culturable) |
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| Research Abstract |
In this study, we investigated the prevalence of non-culturable Legionella species in bath water samples using amoebal co-culture. Legionella species were detected by amoebal co-culture method from 71 water samples. A total of 63(88.7%) bath water samples were positive by amoebal co-culture with culture, PCR, and qualitative PCR methods, whereas 12(16.9%) water samples were positive by conventional culture method. Our results suggested that bath water samples were highly contaminated by Legionella bacteria including VBNC form.
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