2012 Fiscal Year Final Research Report
Development of a novel three-dimensional culture system for hepatitis viruses
| Project/Area Number |
22790664
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Nagoya City University |
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Principal Investigator |
MURAKAMI Shuko 名古屋市立大学, 大学院・医学研究科, 助教 (50454848)
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| Project Period (FY) |
2010 – 2012
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| Keywords | HBV / 肝細胞 / 3 次元培養 / キメラマウス |
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| Research Abstract |
Investigation of hepatitis B virus (HBV) has been hampered by the absence of accessible in vitro modeling tools. The aim of this study is to design an in vitro model of HBV infection that would support long-term replication. Immortalized normal human hepatocytes, or primary human hepatocytes isolated from chimeric mice with human liver were cultured with three-dimensional (3D) culture system to obtain a tissue-like formation and exposed to HBV-infected serum samples. The HBV-DNA level in supernatants of the culture was higher than 10^5 copies/ml and continued to be detectable for >1 month. The extracellular HBsAg was detected during the culture period. The 3D culture system with hepatocytes provides an accessible in vitro tool to investigate HBV cell entry, replication and susceptibility to new anti-viral agents for multiple HBV genotypes/subgenotypes.
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[Journal Article] Mechanism of the Dependence of Hepatitis B Virus Genotype G on Co-infection with Other Genotypes for Viral Replication2013
Author(s)
Sakamoto T, Tanaka Y, Kani S, Sugiyama M, Watanabe T, Iijima S, Murakami S, Matsuura K, Kusakabe A, Shinkai N, Sugauchi F, Mizokami M
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Journal Title
J Viral. Hepat
Volume: 20 (4)
Pages: e27-36
DOI
Peer Reviewed
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[Journal Article] Hepatitis C virus kinetics by administration of pegylated interferon-lin human and chimeric mice carrying human hepatocytes with variants of the IL28B gene2012
Author(s)
Watanabe T, Sugauchi F, Tanaka Y, Matsuura K, Yatsuhashi H, Murakami S, Iijima S, Iio E, Sugiyama M, Shimada T, Kakuni M, Kohara M, Mizokami M
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Multiple intra-familial transmission patterns of hepatitis B virus genotype D in north-eastern Egypt2012
Author(s)
Ragheb M, Elkady A, Tanaka Y, Murakami S, Attia FM, Hassan AA, Hassan MF, Shedid MM, Abdel Reheem HB, Khan A, Mizokami M.
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Journal Title
J Med Virol
Volume: 84(4)
Pages: 587-95
DOI
Peer Reviewed
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[Presentation] IL28B genetic variants and serum IP-10 level associated with virological response to PEG-IFN/RBV and PEG-IFN/RBV/Telaprevir therapy2012
Author(s)
Matsuura K, Tanaka Y, Watanabe T, Murakami S, Iio E, Endo M, Shinkai N, Fujiwara K, Miyaki T, Nojiri S, Kusakabe A, Orito E, Joh T, Mizokami M
Organizer
The 63rd Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Boston in USA
Year and Date
20121109-13
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[Presentation] 核酸アナログを投与した B 型慢性肝炎患者における interferoninducible protein-10値の動態2012
Author(s)
新海登, 松浦健太郎,渡邊綱正,村上周子,宮木知克,藤原圭,日下部篤宣,飯尾悦子,野尻俊輔,城卓志,田中靖人
Organizer
第20回日本消化器関連学会週間
Place of Presentation
神戸市
Year and Date
20121010-12
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[Presentation] 2 剤,3 剤併用療法における,IP-10の治療前,治療開始早期の推移と治療効果,ウイルス動態に与える影響2012
Author(s)
松浦健太郎, 飯尾悦子,日下部篤宣,新海 登,藤原 圭,宮木知克,野尻俊輔,村上周子,渡邊綱正,折戸悦朗,城卓志,田中靖人
Organizer
第20回日本消化器関連学会週間
Place of Presentation
神戸市
Year and Date
20121010-12
